干旱区生态环境知识资源中心(Arid): Microbial Hotspots in Lithic Microhabitats Inferred from DNA Fractionation and Metagenomics in the Atacama Desert

Arid

DOI	10.3390/microorganisms9051038
	Microbial Hotspots in Lithic Microhabitats Inferred from DNA Fractionation and Metagenomics in the Atacama Desert
	Schulze-Makuch, Dirk; Lipus, Daniel; Arens, Felix L.; Baque, Mickael; Bornemann, Till L., V; de Vera, Jean-Pierre; Flury, Markus; Frosler, Jan; Heinz, Jacob; Hwang, Yunha; Kounaves, Samuel P.; Mangelsdorf, Kai; Meckenstock, Rainer U.; Pannekens, Mark; Probst, Alexander J.; Saenz, Johan S.; Schirmack, Janosch; Schloter, Michael; Schmitt-Kopplin, Philippe; Schneider, Beate; Uhl, Jenny; Vestergaard, Gisle; Valenzuela, Bernardita; Zamorano, Pedro; Wagner, Dirk
通讯作者	Schulze-Makuch, D (corresponding author), Tech Univ Berlin, Ctr Astron & Astrophys, D-10623 Berlin, Germany. ; Schulze-Makuch, D ; Wagner, D (corresponding author), GFZ German Res Ctr Geosci, Sect Geomicrobiol, D-14473 Potsdam, Germany. ; Schulze-Makuch, D (corresponding author), Leibniz Inst Freshwater Ecol & Inland Fisheries I, Dept Expt Limnol, D-16775 Stechlin, Germany. ; Schulze-Makuch, D (corresponding author), Washington State Univ, Sch Environm, Pullman, WA 99163 USA. ; Wagner, D (corresponding author), Univ Potsdam, Inst Geosci, Karl Liebknecht Str 24-25, D-14476 Potsdam, Germany.
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2021
卷号	9 期号:5
英文摘要	The existence of microbial activity hotspots in temperate regions of Earth is driven by soil heterogeneities, especially the temporal and spatial availability of nutrients. Here we investigate whether microbial activity hotspots also exist in lithic microhabitats in one of the most arid regions of the world, the Atacama Desert in Chile. While previous studies evaluated the total DNA fraction to elucidate the microbial communities, we here for the first time use a DNA separation approach on lithic microhabitats, together with metagenomics and other analysis methods (i.e., ATP, PLFA, and metabolite analysis) to specifically gain insights on the living and potentially active microbial community. Our results show that hypolith colonized rocks are microbial hotspots in the desert environment. In contrast, our data do not support such a conclusion for gypsum crust and salt rock environments, because only limited microbial activity could be observed. The hypolith community is dominated by phototrophs, mostly Cyanobacteria and Chloroflexi, at both study sites. The gypsum crusts are dominated by methylotrophs and heterotrophic phototrophs, mostly Chloroflexi, and the salt rocks (halite nodules) by phototrophic and halotolerant endoliths, mostly Cyanobacteria and Archaea. The major environmental constraints in the organic-poor arid and hyperarid Atacama Desert are water availability and UV irradiation, allowing phototrophs and other extremophiles to play a key role in desert ecology.
英文关键词	hyperarid habitat desert ecology extremophile endolith hypolith
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000662452000001
WOS关键词	HYPER-ARID ZONE ; EXTRACELLULAR DNA ; HOT MOMENTS ; COMMUNITY STRUCTURE ; RAPID METHOD ; FATTY-ACIDS ; SP-NOV. ; PHOTOSYNTHESIS ; COLONIZATION ; EXTRACTION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351146
作者单位	[Schulze-Makuch, Dirk; Arens, Felix L.; Heinz, Jacob; Hwang, Yunha; Schirmack, Janosch] Tech Univ Berlin, Ctr Astron & Astrophys, D-10623 Berlin, Germany; [Schulze-Makuch, Dirk; Lipus, Daniel; Schneider, Beate; Wagner, Dirk] GFZ German Res Ctr Geosci, Sect Geomicrobiol, D-14473 Potsdam, Germany; [Schulze-Makuch, Dirk] Leibniz Inst Freshwater Ecol & Inland Fisheries I, Dept Expt Limnol, D-16775 Stechlin, Germany; [Schulze-Makuch, Dirk] Washington State Univ, Sch Environm, Pullman, WA 99163 USA; [Baque, Mickael] German Aerosp Ctr DLR, Inst Planetary Res, D-12489 Berlin, Germany; [Bornemann, Till L., V; Frosler, Jan; Meckenstock, Rainer U.; Pannekens, Mark; Probst, Alexander J.] Univ Duisburg Essen, Dept Chem, Environm Microbiol & Biotechnol, D-45141 Essen, Germany; [de Vera, Jean-Pierre] German Aerosp Ctr DLR, Micrograv User Support Ctr MUSC, D-51147 Cologne, Germany; [Flury, Markus] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA; [Flury, Markus] Washington State Univ, Dept Crop & Soil S...
推荐引用方式 GB/T 7714	Schulze-Makuch, Dirk,Lipus, Daniel,Arens, Felix L.,et al. Microbial Hotspots in Lithic Microhabitats Inferred from DNA Fractionation and Metagenomics in the Atacama Desert[J],2021,9(5).
APA	Schulze-Makuch, Dirk.,Lipus, Daniel.,Arens, Felix L..,Baque, Mickael.,Bornemann, Till L., V.,...&Wagner, Dirk.(2021).Microbial Hotspots in Lithic Microhabitats Inferred from DNA Fractionation and Metagenomics in the Atacama Desert.MICROORGANISMS,9(5).
MLA	Schulze-Makuch, Dirk,et al."Microbial Hotspots in Lithic Microhabitats Inferred from DNA Fractionation and Metagenomics in the Atacama Desert".MICROORGANISMS 9.5(2021).