干旱区生态环境知识资源中心(Arid): Phosphate-Arsenic Interactions in Halophilic Microorganisms of the Microbial Mat from Laguna Tebenquiche: from the Microenvironment to the Genomes

Arid

DOI	10.1007/s00248-020-01673-9
	Phosphate-Arsenic Interactions in Halophilic Microorganisms of the Microbial Mat from Laguna Tebenquiche: from the Microenvironment to the Genomes
	Saona, L. A.; Soria, M.; Duran-Toro, V.; Wormer, L.; Milucka, J.; Castro-Nallar, E.; Meneses, C.; Contreras, M.; Farias, M. E.
通讯作者	Saona, LA ; Farias, ME (corresponding author), Consejo Nacl Invest Cient & Tecn, CCT, Planta Piloto Proc Ind Microbiol PROIMI, Lab Invest Microbiol Lagunas Andinas LIMLA, San Miguel De Tucuman, Tucuman, Argentina. ; Saona, LA (corresponding author), Max Planck Inst Marine Microbiol, Dept Biogeochem, Celsiusstr 1, D-28359 Bremen, Germany.
来源期刊	MICROBIAL ECOLOGY
ISSN	0095-3628
EISSN	1432-184X
出版年	2021
卷号	81 期号:4 页码:941-953
英文摘要	Arsenic (As) is a metalloid present in the earth's crust and widely distributed in the environment. Due to its high concentrations in the Andean valleys and its chemical similarity with phosphorus (P), its biological role in Andean Microbial Ecosystems (AMEs) has begun to be studied. The AMEs are home to extremophilic microbial communities that form microbial mats, evaporites, and microbialites inhabiting Andean lakes, puquios, or salt flats. In this work, we characterize the biological role of As and the effect of phosphate in AMEs from the Laguna Tebenquiche (Atacama Desert, Chile). Using micro X-ray fluorescence, the distribution of As in microbial mat samples was mapped. Taxonomic and inferred functional profiles were obtained from enriched cultures of microbial mats incubated under As stress and different phosphate conditions. Additionally, representative microorganisms highly resistant to As and able to grow under low phosphate concentration were isolated and studied physiologically. Finally, the genomes of the isolated Salicola sp. and Halorubrum sp. were sequenced to analyze genes related to both phosphate metabolism and As resistance. The results revealed As as a key component of the microbial mat ecosystem: (i) As was distributed across all sections of the microbial mat and represented a significant weight percentage of the mat (0.17 %) in comparison with P (0.40%); (ii) Low phosphate concentration drastically changed the microbial community in microbial mat samples incubated under high salinity and high As concentrations; (iii) Archaea and Bacteria isolated from the microbial mat were highly resistant to arsenate (up to 500 mM), even under low phosphate concentration; (iv) The genomes of the two isolates were predicted to contain key genes in As metabolism (aioAB and arsC/acr3) and the genes predicted to encode the phosphate-specific transport operon (pstSCAB-phoU) are next to the arsC gene, suggesting a functional relationship between these two elements.
英文关键词	AMEs Arsenic Phosphate Microbial mat arsC pstSCAB-phoU
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000604147300001
WOS关键词	16S RIBOSOMAL-RNA ; MONO LAKE ; BACTERIAL DIVERSITY ; HYPERARID CORE ; ATACAMA DESERT ; SP NOV. ; PHOSPHORUS ; COMMUNITIES ; METABOLISM ; SEDIMENTS
WOS类目	Ecology ; Marine & Freshwater Biology ; Microbiology
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/351135
作者单位	[Saona, L. A.; Soria, M.; Farias, M. E.] Consejo Nacl Invest Cient & Tecn, CCT, Planta Piloto Proc Ind Microbiol PROIMI, Lab Invest Microbiol Lagunas Andinas LIMLA, San Miguel De Tucuman, Tucuman, Argentina; [Saona, L. A.; Milucka, J.] Max Planck Inst Marine Microbiol, Dept Biogeochem, Celsiusstr 1, D-28359 Bremen, Germany; [Duran-Toro, V.] Univ Bremen, MARUM Ctr Marine Environm Sci, Hydrothermal Geomicrobiol Grp, Bremen, Germany; [Wormer, L.] Univ Bremen, MARUM Ctr Marine Environm Sci, Organ Geochem Grp, Leobener Str 8, D-28359 Bremen, Germany; [Castro-Nallar, E.] Univ Andres Bello, Ctr Bioinformat & Integrat Biol, Fac Ciencias Vida, Santiago, Chile; [Meneses, C.] Univ Andres Bello, FONDAP Ctr Genome Regulat, Ctr Biotecnol Vegetal CBV, Santiago, Chile; [Contreras, M.] Ctr Ecol Aplicada, Santiago, Chile
推荐引用方式 GB/T 7714	Saona, L. A.,Soria, M.,Duran-Toro, V.,et al. Phosphate-Arsenic Interactions in Halophilic Microorganisms of the Microbial Mat from Laguna Tebenquiche: from the Microenvironment to the Genomes[J],2021,81(4):941-953.
APA	Saona, L. A..,Soria, M..,Duran-Toro, V..,Wormer, L..,Milucka, J..,...&Farias, M. E..(2021).Phosphate-Arsenic Interactions in Halophilic Microorganisms of the Microbial Mat from Laguna Tebenquiche: from the Microenvironment to the Genomes.MICROBIAL ECOLOGY,81(4),941-953.
MLA	Saona, L. A.,et al."Phosphate-Arsenic Interactions in Halophilic Microorganisms of the Microbial Mat from Laguna Tebenquiche: from the Microenvironment to the Genomes".MICROBIAL ECOLOGY 81.4(2021):941-953.