干旱区生态环境知识资源中心(Arid): Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert

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DOI	10.1016/j.jpha.2020.06.004
	Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert
	Wang, Ting; Li, Feina; Lu, Qinpei; Wu, Gang; Jiang, Zhongke; Liu, Shaowei; Habden, Xugela; Razumova, Elizaveta A.; Osterman, Ilya A.; Sergiev, Petr, V; Dontsova, Olga A.; Hu, Xinxin; You, Xuefu; Sun, Chenghang
通讯作者	Sun, CH (corresponding author), Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Beijing 100050, Peoples R China.
来源期刊	JOURNAL OF PHARMACEUTICAL ANALYSIS
ISSN	2095-1779
EISSN	2214-0883
出版年	2021
卷号	11 期号:2 页码:241-250
英文摘要	Three hundred and twenty endophytic actinobacterial strains were isolated from psammophytes collected from Taklamakan Desert and identified. Among them, three strains already had been identified as new species of two genera and sixteen isolates showed relatively low 16S rRNA similarities < 98.6% to validly described species. Seventy-five of the isolates were selected as representative strains to screen antibacterial activity and mechanism. Forty-seven strains showed antagonistic activity against at least one of the indicator bacteria. Two Streptomyces strains produced bioactive compounds inducing DNA damage, and two Streptomyces strains produced bioactive compounds with inhibitory activity on protein biosynthesis. Notably, the strain Streptomyces sp. 8P21H-1 that demonstrated both strong antibacterial activity and inhibitory activity on protein biosynthesis was prioritized for exploring new antibiotics. Under the strategy of integrating genetics-based discovery program and MS/MS-based molecular networking, two new streptogramin-type antibiotics, i.e., acetyl-griseoviridin and desulphurizing gri-seoviridin, along with known griseoviridin, were isolated from the culture broth of strain 8P21H-1. Their chemical structures were determined by HR-MS, and 1D and 2D NMR. Desulphurizing griseoviridin and griseoviridin exhibited antibacterial activities by inhibiting translation. (c) 2020 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
英文关键词	Taklamakan desert Endophytic actinobacteria Novelty Diversity Streptogramin-type antibiotic
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000647667700011
WOS关键词	FLUORESCENT-PROTEIN REPORTER ; MOLECULAR NETWORKING ; GENE CLUSTERS ; SEQUENCE ; IDENTIFICATION ; GRISEOVIRIDIN ; METABOLISM ; HABITATS ; RIBOSOME ; PLATFORM
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350963
作者单位	[Wang, Ting; Li, Feina; Lu, Qinpei; Wu, Gang; Jiang, Zhongke; Liu, Shaowei; Hu, Xinxin; You, Xuefu; Sun, Chenghang] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Beijing 100050, Peoples R China; [Wang, Ting; Li, Feina; Lu, Qinpei; Wu, Gang; Jiang, Zhongke; Liu, Shaowei; Hu, Xinxin; You, Xuefu; Sun, Chenghang] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Beijing Key Lab Antimicrobial Agents, Beijing 100050, Peoples R China; [Habden, Xugela] Xinjiang Normal Univ, Coll Life Sci, Urumqi 830054, Peoples R China; [Razumova, Elizaveta A.; Osterman, Ilya A.; Sergiev, Petr, V; Dontsova, Olga A.] Lomonosov Moscow State Univ, Moscow 119992, Russia; [Osterman, Ilya A.; Sergiev, Petr, V; Dontsova, Olga A.] Skolkovo Inst Sci & Technol, Ctr Life Sci, Moscow 143025, Russia; [Dontsova, Olga A.] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 119992, Russia
推荐引用方式 GB/T 7714	Wang, Ting,Li, Feina,Lu, Qinpei,et al. Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert[J],2021,11(2):241-250.
APA	Wang, Ting.,Li, Feina.,Lu, Qinpei.,Wu, Gang.,Jiang, Zhongke.,...&Sun, Chenghang.(2021).Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert.JOURNAL OF PHARMACEUTICAL ANALYSIS,11(2),241-250.
MLA	Wang, Ting,et al."Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert".JOURNAL OF PHARMACEUTICAL ANALYSIS 11.2(2021):241-250.