干旱区生态环境知识资源中心(Arid): Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities

Arid

DOI	10.3390/jof7030213
	Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities
	Selbmann, Laura; Stoppiello, Gerardo A.; Onofri, Silvano; Stajich, Jason E.; Coleine, Claudia
通讯作者	Coleine, C (corresponding author), Univ Tuscia, Dept Ecol & Biol Sci, I-01100 Viterbo, Italy. ; Stajich, JE (corresponding author), Univ Calif Riverside, Dept Microbiol & Plant Pathol, Riverside, CA 92521 USA.
来源期刊	JOURNAL OF FUNGI
EISSN	2309-608X
出版年	2021
卷号	7 期号:3
英文摘要	In the harshest environmental conditions of the Antarctic desert, normally incompatible with active life, microbes are adapted to exploit the cryptoendolithic habitat (i.e., pore spaces of rocks) and represent the predominant life-forms. In the rocky niche, microbes take advantage of the thermal buffering, physical stability, protection against UV radiation, excessive solar radiation, and water retention-of paramount importance in one of the driest environments on Earth. In this work, high-throughput sequencing and culture-dependent approaches have been combined, for the first time, to untangle the diversity and distribution of black fungi in the Antarctic cryptoendolithic microbial communities, hosting some of the most extreme-tolerant microorganisms. Rock samples were collected in a vast area, along an altitudinal gradient and opposite sun exposure-known to influence microbial diversity-with the aim to compare and integrate results gained with the two approaches. Among black fungi, Friedmanniomyces endolithicus was confirmed as the most abundant taxon. Despite the much stronger power of the high-throughput sequencing, several species were not retrieved with DNA sequencing and were detectable by cultivation only. We conclude that both culture-dependent and -independent analyses are needed for a complete overview of black fungi diversity. The reason why some species remain undetectable with molecular methods are speculated upon. The effect of environmental parameters such as sun exposure on relative abundance was clearer if based on the wider biodiversity detected with the molecular approach.
英文关键词	Antarctica cryptoendolithic communities metabarcoding black fungi extremophiles
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000633789100001
WOS类目	Microbiology ; Mycology
WOS研究方向	Microbiology ; Mycology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350823
作者单位	[Selbmann, Laura; Stoppiello, Gerardo A.; Onofri, Silvano; Coleine, Claudia] Univ Tuscia, Dept Ecol & Biol Sci, I-01100 Viterbo, Italy; [Selbmann, Laura] Italian Antarctic Natl Museum MNA, Mycol Sect, I-16121 Genoa, Italy; [Stajich, Jason E.] Univ Calif Riverside, Dept Microbiol & Plant Pathol, Riverside, CA 92521 USA
推荐引用方式 GB/T 7714	Selbmann, Laura,Stoppiello, Gerardo A.,Onofri, Silvano,et al. Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities[J],2021,7(3).
APA	Selbmann, Laura,Stoppiello, Gerardo A.,Onofri, Silvano,Stajich, Jason E.,&Coleine, Claudia.(2021).Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities.JOURNAL OF FUNGI,7(3).
MLA	Selbmann, Laura,et al."Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities".JOURNAL OF FUNGI 7.3(2021).