干旱区生态环境知识资源中心(Arid): Determination of gamithromycin residues in eggs, milk and edible tissue of food-producing animals by solid phase extraction combined with ultrahigh-performance liquid chromatography-tandem mass spectrometry

Arid

DOI	10.1016/j.jchromb.2021.122637
	Determination of gamithromycin residues in eggs, milk and edible tissue of food-producing animals by solid phase extraction combined with ultrahigh-performance liquid chromatography-tandem mass spectrometry
	Yan, Yonghuan; Zhang, Haichao; Ai, Lianfeng; Kang, Weijun; Lian, Kaoqi; Wang, Jing
通讯作者	Lian, KQ (corresponding author), Hebei Med Univ, Sch Publ Hlth, Dept Sanit Inspect, Shijiazhuang 050017, Hebei, Peoples R China. ; Ai, LF (corresponding author), Technol Ctr Shijiazhuang Customs, Shijiazhuang 050051, Hebei, Peoples R China.
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2021
卷号	1171
英文摘要	A high throughput method was developed and validated for the quantitation of gamithromycin residues in eggs, milk and animal tissues (leg muscle, kidney, liver and fat) of different species and genera. This was undertaken using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The samples were extracted with acetonitrile and purified using an Oasis MCX solid phase extraction cartridge. Subsequently, a C18 column was used for chromatographic separation using acetonitrile and 0.1% formic acid as the mobile phase. LC-MS/MS in positive ESI and multiple reaction monitoring mode with gamithromycin-D4 as the internal standard was used for detection and quantification of gamithromycin. The method was successfully calibrated in the range of 1.0?200 ?g/kg. The limit of detection (LOD) and limit of quantification (LOQ) for gamithromycin was 0.30?0.40 ?g/kg and 0.80 ? 1.0 ?g/kg, respectively. The average recoveries of the analyte fortified at three levels ranged from 84.2% to 115.9%, with a relative standard deviation <10%. The proposed method has been successfully used to monitor real samples, and shown to be sensitive, rapid, and convenient. Hence, this method could be used for regulatory purposes to screen for the presence of gamithromycin residues in eggs, milk and target tissues.
英文关键词	Gamithromycin UHPLC-MS MS Solid phase extraction Eggs Milk Animal tissues
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000640384000022
WOS关键词	BOVINE RESPIRATORY-DISEASE ; COMPARATIVE EFFICACY ; PHARMACOKINETICS ; ANTIBIOTICS
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350752
作者单位	[Yan, Yonghuan; Kang, Weijun; Lian, Kaoqi] Hebei Med Univ, Sch Publ Hlth, Dept Sanit Inspect, Shijiazhuang 050017, Hebei, Peoples R China; [Zhang, Haichao; Ai, Lianfeng; Wang, Jing] Technol Ctr Shijiazhuang Customs, Shijiazhuang 050051, Hebei, Peoples R China; [Lian, Kaoqi] Hebei Med Univ, Hebei Prov Key Lab Environm & Human Hlth, Shijiazhuang 050017, Hebei, Peoples R China
推荐引用方式 GB/T 7714	Yan, Yonghuan,Zhang, Haichao,Ai, Lianfeng,et al. Determination of gamithromycin residues in eggs, milk and edible tissue of food-producing animals by solid phase extraction combined with ultrahigh-performance liquid chromatography-tandem mass spectrometry[J],2021,1171.
APA	Yan, Yonghuan,Zhang, Haichao,Ai, Lianfeng,Kang, Weijun,Lian, Kaoqi,&Wang, Jing.(2021).Determination of gamithromycin residues in eggs, milk and edible tissue of food-producing animals by solid phase extraction combined with ultrahigh-performance liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1171.
MLA	Yan, Yonghuan,et al."Determination of gamithromycin residues in eggs, milk and edible tissue of food-producing animals by solid phase extraction combined with ultrahigh-performance liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1171(2021).