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DOI | 10.1007/s10815-021-02161-w |
New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause | |
Asiabi, Parinaz; David, Clara; Camboni, Alessandra; Marbaix, Etienne; Dolmans, Marie-Madeleine; Amorim, Christiani A. | |
通讯作者 | Amorim, CA (corresponding author), Catholic Univ Louvain, Inst Rech Expt & Clin, Pole Rech Gynecol, Ave Mounier 52,Bte B1-52-02, B-1200 Brussels, Belgium. |
来源期刊 | JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
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ISSN | 1058-0468 |
EISSN | 1573-7330 |
出版年 | 2021 |
卷号 | 38期号:6页码:1387-1403 |
英文摘要 | Research question Are glioma-associated oncogene homolog 1, 2, and 3 (GLI1, 2, and 3) and protein patched homolog 1 (PTCH1) specific markers for precursor theca cells in human ovaries as in mouse ovaries? Design To study the GDF9-HH-GLI pathway and assess whether GLI1 and 3 and PTCH1 are specific markers for precursor theca cells in the human ovary, growth differentiation factor 9 (GDF9), Indian Hedgehog (IHH), Desert Hedgehog (DHH), Sonic Hedgehog (SHH), PTCH1 and GLI1, 2 and 3 were investigated in fetal (n=9), prepubertal (n=9), reproductive-age (n=15), and postmenopausal (n=8) human ovarian tissue. Immunohistochemistry against GDF9, IHH, DHH, SHH, PTCH1, GLI1, GLI2, and GLI3 was performed on human ovarian tissue sections fixed in 4% formaldehyde and embedded in paraffin. Western blotting was carried out on extracted proteins from the same samples used in the previous step to prove the antibodies' specificity. The quantitative real-time polymerase chain reaction was performed to identify mRNA levels for Gdf9, Ihh, Gli1, Gli2, and Gli3 in menopausal ovaries. Results Our results showed that, in contrast to mice, all studied proteins were expressed in primordial follicles of fetal, prepubertal, and reproductive-age human ovaries and stromal cells of reproductive-age and postmenopausal ovaries. Intriguingly, Gdf9, Ihh, and Gli3 mRNA, but not Gli1 and 2, was detected in postmenopausal ovaries. Moreover, GLI1, GLI3, and PTCH1 are not limited to a specific population of cells. They were spread throughout the organ, which means they are not specific markers for precursor theca cells in human ovaries. Conclusion These results could provide a basis for understanding how this pathway modulates follicle development and ovarian cell steroidogenesis in human ovaries. |
英文关键词 | Growth differentiation factor 9 Hedgehog ligands PTCH1 receptor Precursor theca cells Glioma-associated oncogene homolog 1 2 and 3 |
类型 | Article |
语种 | 英语 |
收录类别 | SCI-E |
WOS记录号 | WOS:000633333900001 |
WOS类目 | Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology |
WOS研究方向 | Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/350736 |
作者单位 | [Asiabi, Parinaz; David, Clara; Camboni, Alessandra; Dolmans, Marie-Madeleine; Amorim, Christiani A.] Catholic Univ Louvain, Inst Rech Expt & Clin, Pole Rech Gynecol, Ave Mounier 52,Bte B1-52-02, B-1200 Brussels, Belgium; [Marbaix, Etienne] Clin Univ St Luc, Gynecol & Androl Dept, B-1200 Brussels, Belgium; [Dolmans, Marie-Madeleine] Clin Univ St Luc, Dept Pathol, Brussels, Belgium |
推荐引用方式 GB/T 7714 | Asiabi, Parinaz,David, Clara,Camboni, Alessandra,et al. New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause[J],2021,38(6):1387-1403. |
APA | Asiabi, Parinaz,David, Clara,Camboni, Alessandra,Marbaix, Etienne,Dolmans, Marie-Madeleine,&Amorim, Christiani A..(2021).New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause.JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,38(6),1387-1403. |
MLA | Asiabi, Parinaz,et al."New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause".JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 38.6(2021):1387-1403. |
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