干旱区生态环境知识资源中心(Arid): New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause

Arid

DOI	10.1007/s10815-021-02161-w
	New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause
	Asiabi, Parinaz; David, Clara; Camboni, Alessandra; Marbaix, Etienne; Dolmans, Marie-Madeleine; Amorim, Christiani A.
通讯作者	Amorim, CA (corresponding author), Catholic Univ Louvain, Inst Rech Expt & Clin, Pole Rech Gynecol, Ave Mounier 52,Bte B1-52-02, B-1200 Brussels, Belgium.
来源期刊	JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
ISSN	1058-0468
EISSN	1573-7330
出版年	2021
卷号	38 期号:6 页码:1387-1403
英文摘要	Research question Are glioma-associated oncogene homolog 1, 2, and 3 (GLI1, 2, and 3) and protein patched homolog 1 (PTCH1) specific markers for precursor theca cells in human ovaries as in mouse ovaries? Design To study the GDF9-HH-GLI pathway and assess whether GLI1 and 3 and PTCH1 are specific markers for precursor theca cells in the human ovary, growth differentiation factor 9 (GDF9), Indian Hedgehog (IHH), Desert Hedgehog (DHH), Sonic Hedgehog (SHH), PTCH1 and GLI1, 2 and 3 were investigated in fetal (n=9), prepubertal (n=9), reproductive-age (n=15), and postmenopausal (n=8) human ovarian tissue. Immunohistochemistry against GDF9, IHH, DHH, SHH, PTCH1, GLI1, GLI2, and GLI3 was performed on human ovarian tissue sections fixed in 4% formaldehyde and embedded in paraffin. Western blotting was carried out on extracted proteins from the same samples used in the previous step to prove the antibodies' specificity. The quantitative real-time polymerase chain reaction was performed to identify mRNA levels for Gdf9, Ihh, Gli1, Gli2, and Gli3 in menopausal ovaries. Results Our results showed that, in contrast to mice, all studied proteins were expressed in primordial follicles of fetal, prepubertal, and reproductive-age human ovaries and stromal cells of reproductive-age and postmenopausal ovaries. Intriguingly, Gdf9, Ihh, and Gli3 mRNA, but not Gli1 and 2, was detected in postmenopausal ovaries. Moreover, GLI1, GLI3, and PTCH1 are not limited to a specific population of cells. They were spread throughout the organ, which means they are not specific markers for precursor theca cells in human ovaries. Conclusion These results could provide a basis for understanding how this pathway modulates follicle development and ovarian cell steroidogenesis in human ovaries.
英文关键词	Growth differentiation factor 9 Hedgehog ligands PTCH1 receptor Precursor theca cells Glioma-associated oncogene homolog 1 2 and 3
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000633333900001
WOS类目	Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
WOS研究方向	Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350736
作者单位	[Asiabi, Parinaz; David, Clara; Camboni, Alessandra; Dolmans, Marie-Madeleine; Amorim, Christiani A.] Catholic Univ Louvain, Inst Rech Expt & Clin, Pole Rech Gynecol, Ave Mounier 52,Bte B1-52-02, B-1200 Brussels, Belgium; [Marbaix, Etienne] Clin Univ St Luc, Gynecol & Androl Dept, B-1200 Brussels, Belgium; [Dolmans, Marie-Madeleine] Clin Univ St Luc, Dept Pathol, Brussels, Belgium
推荐引用方式 GB/T 7714	Asiabi, Parinaz,David, Clara,Camboni, Alessandra,et al. New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause[J],2021,38(6):1387-1403.
APA	Asiabi, Parinaz,David, Clara,Camboni, Alessandra,Marbaix, Etienne,Dolmans, Marie-Madeleine,&Amorim, Christiani A..(2021).New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause.JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,38(6),1387-1403.
MLA	Asiabi, Parinaz,et al."New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause".JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 38.6(2021):1387-1403.