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DOI10.1007/s10815-021-02161-w
New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause
Asiabi, Parinaz; David, Clara; Camboni, Alessandra; Marbaix, Etienne; Dolmans, Marie-Madeleine; Amorim, Christiani A.
通讯作者Amorim, CA (corresponding author), Catholic Univ Louvain, Inst Rech Expt & Clin, Pole Rech Gynecol, Ave Mounier 52,Bte B1-52-02, B-1200 Brussels, Belgium.
来源期刊JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
ISSN1058-0468
EISSN1573-7330
出版年2021
卷号38期号:6页码:1387-1403
英文摘要Research question Are glioma-associated oncogene homolog 1, 2, and 3 (GLI1, 2, and 3) and protein patched homolog 1 (PTCH1) specific markers for precursor theca cells in human ovaries as in mouse ovaries? Design To study the GDF9-HH-GLI pathway and assess whether GLI1 and 3 and PTCH1 are specific markers for precursor theca cells in the human ovary, growth differentiation factor 9 (GDF9), Indian Hedgehog (IHH), Desert Hedgehog (DHH), Sonic Hedgehog (SHH), PTCH1 and GLI1, 2 and 3 were investigated in fetal (n=9), prepubertal (n=9), reproductive-age (n=15), and postmenopausal (n=8) human ovarian tissue. Immunohistochemistry against GDF9, IHH, DHH, SHH, PTCH1, GLI1, GLI2, and GLI3 was performed on human ovarian tissue sections fixed in 4% formaldehyde and embedded in paraffin. Western blotting was carried out on extracted proteins from the same samples used in the previous step to prove the antibodies' specificity. The quantitative real-time polymerase chain reaction was performed to identify mRNA levels for Gdf9, Ihh, Gli1, Gli2, and Gli3 in menopausal ovaries. Results Our results showed that, in contrast to mice, all studied proteins were expressed in primordial follicles of fetal, prepubertal, and reproductive-age human ovaries and stromal cells of reproductive-age and postmenopausal ovaries. Intriguingly, Gdf9, Ihh, and Gli3 mRNA, but not Gli1 and 2, was detected in postmenopausal ovaries. Moreover, GLI1, GLI3, and PTCH1 are not limited to a specific population of cells. They were spread throughout the organ, which means they are not specific markers for precursor theca cells in human ovaries. Conclusion These results could provide a basis for understanding how this pathway modulates follicle development and ovarian cell steroidogenesis in human ovaries.
英文关键词Growth differentiation factor 9 Hedgehog ligands PTCH1 receptor Precursor theca cells Glioma-associated oncogene homolog 1 2 and 3
类型Article
语种英语
收录类别SCI-E
WOS记录号WOS:000633333900001
WOS类目Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
WOS研究方向Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/350736
作者单位[Asiabi, Parinaz; David, Clara; Camboni, Alessandra; Dolmans, Marie-Madeleine; Amorim, Christiani A.] Catholic Univ Louvain, Inst Rech Expt & Clin, Pole Rech Gynecol, Ave Mounier 52,Bte B1-52-02, B-1200 Brussels, Belgium; [Marbaix, Etienne] Clin Univ St Luc, Gynecol & Androl Dept, B-1200 Brussels, Belgium; [Dolmans, Marie-Madeleine] Clin Univ St Luc, Dept Pathol, Brussels, Belgium
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Asiabi, Parinaz,David, Clara,Camboni, Alessandra,et al. New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause[J],2021,38(6):1387-1403.
APA Asiabi, Parinaz,David, Clara,Camboni, Alessandra,Marbaix, Etienne,Dolmans, Marie-Madeleine,&Amorim, Christiani A..(2021).New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause.JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,38(6),1387-1403.
MLA Asiabi, Parinaz,et al."New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause".JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 38.6(2021):1387-1403.
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