干旱区生态环境知识资源中心(Arid): Cloning, expression and functional analysis of the desert hedgehog (dhh) gene in Chinese tongue sole (Cynoglossus semilaevis)

Arid

DOI	10.1016/j.gep.2020.119163
	Cloning, expression and functional analysis of the desert hedgehog (dhh) gene in Chinese tongue sole (Cynoglossus semilaevis)
	Qi, Qian; Dong, Zhongdian; Zhang, Ning; Wang, Liang; Shao, Changwei; Xu, Wenteng
通讯作者	Xu, WT (corresponding author), Chinese Acad Fishery Sci CAFS, Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China.
来源期刊	GENE EXPRESSION PATTERNS
ISSN	1567-133X
EISSN	1872-7298
出版年	2021
卷号	39
英文摘要	Desert hedgehog (dhh) is a gene that is crucial for spermatogenesis and Leydig cell differentiation, but little is known regarding its influence on gonadal differentiation and development in fish. To understand its function, we cloned and characterized the dhh gene from Cynoglossus semilaevis (csdhh). The full length csdhh cDNA was 2473 bp, including a 1386 bp open reading frame (ORF), a 475 bp 5?-UTR, and a 612 bp 3?-UTR, encoding a predicted protein of 461 amino acid residues. Phylogenetic analysis showed that the putative protein belongs to the hedgehog (HH) family, and contains typical HH-N and HH-C domains. Amino acid sequence analysis revealed that CsDhh shares many features with Dhh analogues in other teleost species. Real-time quantitative PCR showed that csdhh was detected in eight different tissues in male and female tongue sole. During early embryonic development, the relative expression of the csdhh was significantly higher in the neural stage than in other embryonic developmental stages (P < 0.05). csdhh was detected at 20 days after hatching (dah) and at the critical period of male gonadal differentiation (80?95 dah), the relative expression of the csdhh was significantly higher in the male gonads than the female gonads. In 5, 8, and 12 month old gonads, the relative expression of the csdhh was significantly higher in male and pseudo-male than in female fish. The in situ hybridization (ISH) results showed that the hybridization signal was strongly expressed in primary and secondary spermatocytes, spermatids, and sertoli cells of the 1-year-old fish testis, with only weak signal expression in the corresponding ovarian tissue. These results suggest that csdhh is highly conserved in evolution and plays an important role in spermatogenesis in males and pseudo-males.
英文关键词	Cynoglossus semilaevis dhh Expression analysis Gonadal differentiation
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000634881700003
WOS类目	Developmental Biology ; Genetics & Heredity
WOS研究方向	Developmental Biology ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350352
作者单位	[Qi, Qian] Henan Univ Sci & Technol, Luoyang 471000, Peoples R China; [Qi, Qian; Shao, Changwei; Xu, Wenteng] Chinese Acad Fishery Sci CAFS, Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; [Dong, Zhongdian; Zhang, Ning] Guangdong Ocean Univ, Guangdong South China Sea Key Lab Aquaculture Aqu, Zhanjiang 524088, Peoples R China; [Wang, Liang] Yantai Marine Econ Res Inst, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Qi, Qian,Dong, Zhongdian,Zhang, Ning,et al. Cloning, expression and functional analysis of the desert hedgehog (dhh) gene in Chinese tongue sole (Cynoglossus semilaevis)[J],2021,39.
APA	Qi, Qian,Dong, Zhongdian,Zhang, Ning,Wang, Liang,Shao, Changwei,&Xu, Wenteng.(2021).Cloning, expression and functional analysis of the desert hedgehog (dhh) gene in Chinese tongue sole (Cynoglossus semilaevis).GENE EXPRESSION PATTERNS,39.
MLA	Qi, Qian,et al."Cloning, expression and functional analysis of the desert hedgehog (dhh) gene in Chinese tongue sole (Cynoglossus semilaevis)".GENE EXPRESSION PATTERNS 39(2021).