干旱区生态环境知识资源中心(Arid): Changes in Cell Vitality, Phenotype, and Function of Dromedary Camel Leukocytes After Whole Blood Exposure to Heat Stress in vitro

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DOI	10.3389/fvets.2021.647609
	Changes in Cell Vitality, Phenotype, and Function of Dromedary Camel Leukocytes After Whole Blood Exposure to Heat Stress in vitro
	Hussen, Jamal
通讯作者	Hussen, J (corresponding author), King Faisal Univ, Coll Vet Med, Dept Microbiol, Al Hasa, Saudi Arabia.
来源期刊	FRONTIERS IN VETERINARY SCIENCE
EISSN	2297-1769
出版年	2021
卷号	8
英文摘要	The dromedary camel (Camelus dromedarius) is well-adapted to the desert environment with the ability to tolerate increased internal body temperatures rising daily to 41-42 degrees C during extreme hot. This study was undertaken to assess whether in vitro incubation of camel blood at 41 degrees C, simulating conditions of heat stress, differently alters cell vitality, phenotype, and function of leukocytes, compared to incubation at 37 degrees C (normothermia). Using flow cytometry, the cell vitality (necrosis and apoptosis), the expression of several cell markers and adhesion molecules, and the antimicrobial functions of camel leukocytes were analyzed in vitro. The fraction of apoptotic cells within the granulocytes, lymphocytes, and monocytes increased significantly after incubation of camel whole blood at 41 degrees C for 4 h. The higher increase in apoptotic granulocytes and monocytes compared to lymphocytes suggests higher resistance of camel lymphocytes to heat stress. Functionally, incubation of camel blood at 41 degrees C for 4 h enhanced the phagocytosis and ROS production activities of camel neutrophils and monocytes toward S. aureus. Monocytes from camel blood incubated at 41 degrees C for 4 h significantly decreased their expression level of MHC class II molecules with no change in the abundance of CD163, resulting in a CD163(high) MHC-IIlow M2-like macrophage phenotype. In addition, heat stress treatment showed an inhibitory effect on the LPS-induced changes in camel monocytes phenotype. Furthermore, in vitro incubation of camel blood at 41 degrees C reduced the expression of the cell adhesion molecules CD18 and CD11a on neutrophils and monocytes. Collectively, the present study identified some heat-stress-induced phenotypic and functional alterations in camel blood leukocytes, providing a paradigm for comparative immunology in the large animals. The clinical relevance of the observed changes in camel leukocytes for the adaptation of the camel immune response to heat stress conditions needs further in vitro and in vivo studies.
英文关键词	camel (Camelus dromedarius) leukocytes heat stress phagocytosis ROS immunity
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000642429600001
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350340
作者单位	[Hussen, Jamal] King Faisal Univ, Coll Vet Med, Dept Microbiol, Al Hasa, Saudi Arabia
推荐引用方式 GB/T 7714	Hussen, Jamal. Changes in Cell Vitality, Phenotype, and Function of Dromedary Camel Leukocytes After Whole Blood Exposure to Heat Stress in vitro[J],2021,8.
APA	Hussen, Jamal.(2021).Changes in Cell Vitality, Phenotype, and Function of Dromedary Camel Leukocytes After Whole Blood Exposure to Heat Stress in vitro.FRONTIERS IN VETERINARY SCIENCE,8.
MLA	Hussen, Jamal."Changes in Cell Vitality, Phenotype, and Function of Dromedary Camel Leukocytes After Whole Blood Exposure to Heat Stress in vitro".FRONTIERS IN VETERINARY SCIENCE 8(2021).

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