干旱区生态环境知识资源中心(Arid): Biochemical Profiling for Antioxidant and Therapeutic Potential of Pakistani Chickpea (Cicer arietinum L.) Genetic Resource

Arid

DOI	10.3389/fpls.2021.663623
	Biochemical Profiling for Antioxidant and Therapeutic Potential of Pakistani Chickpea (Cicer arietinum L.) Genetic Resource
	Jameel, Saima; Hameed, Amjad; Shah, Tariq Mahmud
通讯作者	Hameed, A (corresponding author), Pakistan Inst Engn & Appl Sci, Nucl Inst Agr, Faisalabad, Pakistan.
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2021
卷号	12
英文摘要	In Pakistan, chickpeas (Cicer arietinum L.) are the largest grown legume crops, especially in desert areas. Along with an excellent source of nutrition, chickpea seeds have discernible medicinal and antioxidant characteristics. The diverse set of 90 chickpea genotypes (66 desi and 24 kabuli) were collected from different research zones in Pakistan, and seed flour was used for biochemical profiling. Genotypes were significantly different (Tukey HSD test, P < 0.05) for the traits under investigation. In non-enzymatic antioxidants, highest seed total phenolic contents (TPC) (34725 +/- 275 mu M/g s. wt.) was found in CM-98 (desi), ascorbic acid (AsA) (69.23 +/- 2.25 mu g/g s. wt.) in WH-3 (desi), and total flavonoid content (TFC) (394.98 +/- 13.06 mu g/mL sample) was detected in WH-11 (desi). In the class of enzymatic antioxidants, the highest seed ascorbate peroxidase (APX) (1680 +/- 40 Units/g s. wt.) was detected in Tamman-2013 (kabuli), peroxidases (POD) (2564.10 +/- 233.10 Units/g s. wt.) activity in CM1235/08 (desi), and superoxide dismutase (SOD) (279.76 +/- 50 Units/g s. wt.) was detected in CH24/11 (desi). Highest seed catalase activity (CAT) (893 +/- 50 Units/g s. wt.) and proline content (272.50 +/- 20.82 mu g/g s. wt.) was detected in an ICC-4951 (desi). In hydrolytic enzymes, the highest activity of esterase (37.05 mu M/min/g s. wt) was found in, CH56/09(Kabuli), protease (11080 +/- 10 Units/g s. wt.) in Karak-2 (desi), and alpha-amylase (213.02 +/- 3.20 mg/g s. wt.) was observed in CH74/08 (kabuli). In other biochemical parameters, the highest seed total oxidant status (TOS) (356 +/- 17.50 mu M/g s. wt.) was detected in CM3457/91 (desi); malondialdehyde (MDA) content (295.74 +/- 3.097 uM/g s. wt.) was observed in CM-2008 (kabuli), and total antioxidant capacity (TAC) (8.36 +/- 0.082 mu M/g s. wt.) was found in CM-72 (desi). In case of pigment analysis, Sheenghar-2000 (desi) depicted highest lycopene (12.579 +/- 0.313 mu g/g s. wt.) and total carotenoids (58.430.23 +/- 0.569 mu g/g s. wt.) contents. For seed therapeutic potential, the highest seed alpha-amylase inhibition (82.33 +/- 8.06%) was observed in CM-88 (desi), while WH-1, WH-6, and ICCV-96030 (desi) depicted the highest value for seed anti-inflammatory potential (78.88 +/- 0.55%). Genotypes with the highest antioxidant and therapeutic potential can be utilized as a natural antioxidant source and in breeding programs aimed at improving these traits in new breeding lines.
英文关键词	desi kabuli hydrolytic enzymes alpha amylase inhibition anti-inflammatory
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000644419000001
WOS关键词	GROWTH-PROMOTING RHIZOBACTERIA ; OXIDATIVE STRESS ; DEFENSE ; DESI ; ASCORBATE ; PEPTIDES ; CAPACITY ; PUNJAB ; LEAVES
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350335
作者单位	[Hameed, Amjad] Pakistan Inst Engn & Appl Sci, Nucl Inst Agr, Faisalabad, Pakistan; Pakistan Inst Engn & Appl Sci, Biol Coll, Faisalabad, Pakistan
推荐引用方式 GB/T 7714	Jameel, Saima,Hameed, Amjad,Shah, Tariq Mahmud. Biochemical Profiling for Antioxidant and Therapeutic Potential of Pakistani Chickpea (Cicer arietinum L.) Genetic Resource[J],2021,12.
APA	Jameel, Saima,Hameed, Amjad,&Shah, Tariq Mahmud.(2021).Biochemical Profiling for Antioxidant and Therapeutic Potential of Pakistani Chickpea (Cicer arietinum L.) Genetic Resource.FRONTIERS IN PLANT SCIENCE,12.
MLA	Jameel, Saima,et al."Biochemical Profiling for Antioxidant and Therapeutic Potential of Pakistani Chickpea (Cicer arietinum L.) Genetic Resource".FRONTIERS IN PLANT SCIENCE 12(2021).