干旱区生态环境知识资源中心(Arid): Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods

Arid

DOI	10.3389/fmicb.2021.630013
	Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods
	Sysoev, Maksim; Groetzinger, Stefan W.; Renn, Dominik; Eppinger, Jorg; Rueping, Magnus; Karan, Ram
通讯作者	Rueping, M ; Karan, R (corresponding author), King Abdullah Univ Sci & Technol KAUST, KAUST Catalysis Ctr KCC, Thuwal, Saudi Arabia. ; Rueping, M (corresponding author), Rhein Westfal TH Aachen, Inst Expt Mol Imaging, Univ Clin, Aachen, Germany.
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Extremophiles are remarkable organisms that thrive in the harshest environments on Earth, such as hydrothermal vents, hypersaline lakes and pools, alkaline soda lakes, deserts, cold oceans, and volcanic areas. These organisms have developed several strategies to overcome environmental stress and nutrient limitations. Thus, they are among the best model organisms to study adaptive mechanisms that lead to stress tolerance. Genetic and structural information derived from extremophiles and extremozymes can be used for bioengineering other nontolerant enzymes. Furthermore, extremophiles can be a valuable resource for novel biotechnological and biomedical products due to their biosynthetic properties. However, understanding life under extreme conditions is challenging due to the difficulties of in vitro cultivation and observation since > 99% of organisms cannot be cultivated. Consequently, only a minor percentage of the potential extremophiles on Earth have been discovered and characterized. Herein, we present a review of culture-independent methods, sequence-based metagenomics (SBM), and single amplified genomes (SAGs) for studying enzymes from extremophiles, with a focus on prokaryotic (archaea and bacteria) microorganisms. Additionally, we provide a comprehensive list of extremozymes discovered via metagenomics and SAGs.
英文关键词	extremophile culture-independent methods metagenomics SAG halophiles thermophiles psychrophile
类型	Review
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000620797200001
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350317
作者单位	[Sysoev, Maksim; Groetzinger, Stefan W.; Renn, Dominik; Eppinger, Jorg; Rueping, Magnus; Karan, Ram] King Abdullah Univ Sci & Technol KAUST, KAUST Catalysis Ctr KCC, Thuwal, Saudi Arabia; [Eppinger, Jorg; Rueping, Magnus] Rhein Westfal TH Aachen, Inst Expt Mol Imaging, Univ Clin, Aachen, Germany
推荐引用方式 GB/T 7714	Sysoev, Maksim,Groetzinger, Stefan W.,Renn, Dominik,et al. Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods[J],2021,12.
APA	Sysoev, Maksim,Groetzinger, Stefan W.,Renn, Dominik,Eppinger, Jorg,Rueping, Magnus,&Karan, Ram.(2021).Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods.FRONTIERS IN MICROBIOLOGY,12.
MLA	Sysoev, Maksim,et al."Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods".FRONTIERS IN MICROBIOLOGY 12(2021).