干旱区生态环境知识资源中心(Arid): Antimicrobial Resistance in Escherichia coli and Enterococcal Isolates From Irrigation Return Flows in a High-Desert Watershed

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DOI	10.3389/fmicb.2021.660697
	Antimicrobial Resistance in Escherichia coli and Enterococcal Isolates From Irrigation Return Flows in a High-Desert Watershed
	Dungan, Robert S.; Bjorneberg, David L.
通讯作者	Dungan, RS (corresponding author), ARS, Northwest Irrigat & Soils Res Lab, USDA, Kimberly, ID 83341 USA.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Irrigation return flows (IRFs) collect surface runoff and subsurface drainage, causing them to have elevated contaminant and bacterial levels, and making them a potential source of pollutants. The purpose of this study was to determine antimicrobial susceptibility among Escherichia coli and enterococcal isolates that were collected from IRFs in a south-central Idaho watershed. Environmental isolates can be a potentially important source of antimicrobial resistance (AMR) and IRFs may be one way resistance genes are transported out of agroecosystems. Water samples were collected from nine IRFs and one background site (canal water from Snake River) on a biweekly basis during 2018. Escherichia coli and enterococci were enumerated via a most probable number (MPN) technique, then subsamples were plated on selective media to obtain isolates. Isolates of E. coli (187) or enterococci (185) were tested for antimicrobial susceptibility using Sensititre broth microdilution plates. For E. coli, 13% (25/187) of isolates were resistant to tetracycline, with fewer numbers being resistant to 13 other antimicrobials, with none resistant to gentamicin. While 75% (141/187) of the E. coli isolates were pan-susceptible, 12 multidrug resistance (MDR) patterns with 17 isolates exhibiting resistance to up to seven drug classes (10 antimicrobials). For the enterococcal species, only 9% (16/185) of isolates were pan-susceptible and the single highest resistance was to lincomycin (138/185; 75%) followed by nitrofurantoin (56/185; 30%) and quinupristin/dalfopristin (34/185; 18%). In addition, 13 enterococcal isolates belonging to Enterococcus faecalis, Enterococcus faecium, Enterococcus casseliflavus, and Enterococcus thailandicus, were determined to be MDR to up to six different antimicrobial drug classes. None of the enterococcal isolates were resistant to gentamycin, linezolid, tigecycline, and vancomycin.
英文关键词	antimicrobial susceptibility Escherichia coli enterococci irrigation return flow fecal indicator bacteria watershed
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000654002400001
WOS关键词	ANTIBIOTIC-RESISTANCE ; FECAL-COLIFORMS ; SOURCE TRACKING ; GENES ; IDENTIFICATION ; PREVALENCE ; BACTERIA ; URBAN ; SPP. ; FOOD
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350316
作者单位	[Dungan, Robert S.; Bjorneberg, David L.] ARS, Northwest Irrigat & Soils Res Lab, USDA, Kimberly, ID 83341 USA
推荐引用方式 GB/T 7714	Dungan, Robert S.,Bjorneberg, David L.. Antimicrobial Resistance in Escherichia coli and Enterococcal Isolates From Irrigation Return Flows in a High-Desert Watershed[J],2021,12.
APA	Dungan, Robert S.,&Bjorneberg, David L..(2021).Antimicrobial Resistance in Escherichia coli and Enterococcal Isolates From Irrigation Return Flows in a High-Desert Watershed.FRONTIERS IN MICROBIOLOGY,12.
MLA	Dungan, Robert S.,et al."Antimicrobial Resistance in Escherichia coli and Enterococcal Isolates From Irrigation Return Flows in a High-Desert Watershed".FRONTIERS IN MICROBIOLOGY 12(2021).