干旱区生态环境知识资源中心(Arid): Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China

Arid

DOI	10.3389/fmicb.2021.633141
	Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China
	Pang, Jinfeng; Palmer, Marike; Sun, Henry J.; Seymour, Cale O.; Zhang, Ling; Hedlund, Brian P.; Zeng, Fanjiang
通讯作者	Zeng, FJ (corresponding author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi, Peoples R China. ; Zeng, FJ (corresponding author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Xinjiang Key Lab Desert Plant Roots Ecol & Vegeta, Urumqi, Peoples R China. ; Zeng, FJ (corresponding author), Cele Natl Stn Observat & Res Desert Grassland Eco, Cele, Peoples R China. ; Zeng, FJ (corresponding author), Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China. ; Hedlund, BP (corresponding author), Univ Nevada, Sch Life Sci, Las Vegas, NV 89154 USA. ; Hedlund, BP (corresponding author), Nevada Inst Personalized Med, Las Vegas, NV 89154 USA.
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Bacteria in root nodules of legumes play important roles in promoting plant growth. In this study, we investigated root nodule-associated bacteria isolated from leguminous plants along an elevation gradient on the northern slope of the Kunlun Mountains, China, using a cultivation approach. In total, 300 isolates were obtained from seven legume species within six ecological zones. Isolates were identified based on 16S rRNA gene phylogenetic analysis and potential rhizobia were further identified using a recA gene phylogeny. Among the isolates, Bacillales (particularly Bacillus) were the dominant isolates from all host legumes and all elevations (63.5%), followed by Rhizobiales (13%) and Pseudomonadales (11.7%). Less than 3% of the isolates belonged to Burkholderiales, Paenibacillales, Enterobacteriales, Actinomycetales, Sphingomonadales, Xanthomonadales, Chitinophagales, Brevibacillales, Staphylococcales, or Mycobacteriales. A few elevation-specific patterns emerged within the Bacillales and Pseudomonadales. For example, isolates related to the psychrotroph Bacillus psychrosaccharolyticus were only isolated from the highest elevation sites (>3,500 m) whereas those related to the mesophile Bacillus endophyticus were only isolated from lowest elevation sites (1,350 m), suggestive of a role of soil temperature in their distribution. Similarly, isolates related to Pseudomonas brassicacearum were the dominant Pseudomonadales isolates, but they were only isolated from middle and low elevations (<3,200 m). A total of 39 isolates belonged to the Rhizobiales, 36 of which were confirmed to the genus level using the recA gene. In all, Rhizobiales isolates were obtained from five different host legumes spanning the entire elevation gradient. Those from the low-elevation Qira Desert-Oasis Transition Zone (1,350-1,960 m) suggested some patterns of host preference. For example, most isolates from Albizia julibrissin formed a monophyletic group related to Rhizobium lemnae and most from Alhagi sparsifolia were closely related to Ensifer kummerowiae. In general, this study shows that most bacteria associated with root nodules of legumes are widely distributed in distinct ecological zones within a single geographic region but suggests that both climate and host interactions may influence their distributions.
英文关键词	microbial diversity endophytes root nodules legumes (Fabaceae) elevation Kunlun Mountains
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000623626500001
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	中国科学院新疆生态与地理研究所 ; Desert Research Institute
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350311
作者单位	[Pang, Jinfeng; Zhang, Ling; Zeng, Fanjiang] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi, Peoples R China; [Pang, Jinfeng; Zhang, Ling; Zeng, Fanjiang] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Xinjiang Key Lab Desert Plant Roots Ecol & Vegeta, Urumqi, Peoples R China; [Pang, Jinfeng; Zhang, Ling; Zeng, Fanjiang] Cele Natl Stn Observat & Res Desert Grassland Eco, Cele, Peoples R China; [Pang, Jinfeng; Zhang, Ling; Zeng, Fanjiang] Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China; [Pang, Jinfeng; Palmer, Marike; Seymour, Cale O.; Hedlund, Brian P.] Univ Nevada, Sch Life Sci, Las Vegas, NV 89154 USA; [Sun, Henry J.] Desert Res Inst, Las Vegas, NV USA; [Hedlund, Brian P.] Nevada Inst Personalized Med, Las Vegas, NV 89154 USA
推荐引用方式 GB/T 7714	Pang, Jinfeng,Palmer, Marike,Sun, Henry J.,et al. Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China[J]. 中国科学院新疆生态与地理研究所, Desert Research Institute,2021,12.
APA	Pang, Jinfeng.,Palmer, Marike.,Sun, Henry J..,Seymour, Cale O..,Zhang, Ling.,...&Zeng, Fanjiang.(2021).Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China.FRONTIERS IN MICROBIOLOGY,12.
MLA	Pang, Jinfeng,et al."Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China".FRONTIERS IN MICROBIOLOGY 12(2021).