干旱区生态环境知识资源中心(Arid): Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Arid

DOI	10.3389/fmicb.2021.604999
	Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert
	Liu, Shaowei; Wang, Ting; Lu, Qinpei; Li, Feina; Wu, Gang; Jiang, Zhongke; Habden, Xugela; Liu, Lin; Zhang, Xiaolin; Lukianov, Dmitry A.; Osterman, Ilya A.; Sergiev, Petr V.; Dontsova, Olga A.; Sun, Chenghang
通讯作者	Sun, CH (corresponding author), Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Dept Microbial Chem, Beijing, Peoples R China. ; Sun, CH (corresponding author), Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Beijing Key Lab Antimicrobial Agents, Beijing, Peoples R China.
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Taklamakan desert is known as the largest dunefield in China and as the second largest shifting sand desert in the world. Although with long history and glorious culture, the Taklamakan desert remains largely unexplored and numerous microorganisms have not been harvested in culture or taxonomically identified yet. The main objective of this study is to explore the diversity, novelty, and pharmacological potential of the cultivable actinomycetes from soil samples at various sites along the Alar-Hotan desert highway in the Taklamakan desert. A total of 590 actinobacterial strains were recovered by the culture-dependent approach. Phylogenetic analysis based on 16S ribosomal RNA (rRNA) gene sequences unveiled a significant level of actinobacterial diversity with 55 genera distributed in 27 families of 12 orders. Thirty-six strains showed relatively low 16S rRNA similarities (<98.65%) with validly described species, among which four strains had already been characterized as novel taxa by our previous research. One hundred and forty-six actinobacterial isolates were selected as representatives to evaluate the antibacterial activities and mechanism of action by the paper-disk diffusion method and a double fluorescent protein reporter pDualrep2 system, respectively. A total of 61 isolates exhibited antagonistic activity against the tested ESKAPE pathogens, among which seven strains could produce bioactive metabolites either to be able to block translation machinery or to induce SOS-response in the pDualrep2 system. Notably, Saccharothrix sp. 16Sb2-4, harboring a promising antibacterial potential with the mechanism of interfering with protein translation, was analyzed in detail to gain deeper insights into its bioactive metabolites. Through ultra-performance liquid chromatography (UPLC)-quadrupole time-of-flight (QToF)-MS/MS based molecular networking analysis and databases identification, four families of compounds (1-16) were putatively identified. Subsequent bioassay-guided separation resulted in purification of four 16-membered macrolide antibiotics, aldgamycin H (8), aldgamycin K (9), aldgamycin G (10), and swalpamycin B (11), and their structures were elucidated by HR-electrospray ionization source (ESI)-MS and NMR spectroscopy. All compounds 8-11 displayed antibacterial activities by inhibiting protein synthesis in the pDualrep2 system. In conclusion, this work demonstrates that Taklamakan desert is a potentially unique reservoir of versatile actinobacteria, which can be a promising source for discovery of novel species and diverse bioactive compounds.
英文关键词	actinobacteria diversity macrolides Taklamakan desert novel species antibacterial metabolites
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000634271500001
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350310
作者单位	[Liu, Shaowei; Wang, Ting; Lu, Qinpei; Li, Feina; Wu, Gang; Jiang, Zhongke; Sun, Chenghang] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Dept Microbial Chem, Beijing, Peoples R China; [Liu, Shaowei; Sun, Chenghang] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Beijing Key Lab Antimicrobial Agents, Beijing, Peoples R China; [Habden, Xugela] Xinjiang Normal Univ, Coll Life Sci, Urumqi, Peoples R China; [Liu, Lin] China Pharmaceut Univ, Sch Tradit Chinese Pharm, Nanjing, Peoples R China; [Zhang, Xiaolin] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing, Peoples R China; [Lukianov, Dmitry A.; Osterman, Ilya A.; Sergiev, Petr V.; Dontsova, Olga A.] Skolkovo Inst Sci & Technol, Ctr Life Sci, Moscow, Russia; [Osterman, Ilya A.; Sergiev, Petr V.; Dontsova, Olga A.] Lomonosov Moscow State Univ, AN Belozersky Inst Physicochem Biol, Dept Chem, Moscow, Russia; [Dontsova, Olga A.] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
推荐引用方式 GB/T 7714	Liu, Shaowei,Wang, Ting,Lu, Qinpei,et al. Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert[J],2021,12.
APA	Liu, Shaowei.,Wang, Ting.,Lu, Qinpei.,Li, Feina.,Wu, Gang.,...&Sun, Chenghang.(2021).Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert.FRONTIERS IN MICROBIOLOGY,12.
MLA	Liu, Shaowei,et al."Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert".FRONTIERS IN MICROBIOLOGY 12(2021).