干旱区生态环境知识资源中心(Arid): Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water

Arid

DOI	10.3389/fevo.2021.613200
	Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water
	Wilcox, Taylor M.; Caragiulo, Anthony; Dysthe, Joseph C.; Franklin, Thomas W.; Mason, Daniel H.; McKelvey, Kevin S.; Zarn, Katherine E.; Schwartz, Michael K.
通讯作者	Wilcox, TM (corresponding author), US Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, USDA, Missoula, MT 59804 USA.
来源期刊	FRONTIERS IN ECOLOGY AND EVOLUTION
ISSN	2296-701X
出版年	2021
卷号	9
英文摘要	Jaguar (Panthera onca) are of conservation concern and occur at very low densities in the northern portion of their range in northern Mexico and the southwestern United States. Environmental DNA sampling to detect genetic material from drinking water may be an effective approach for jaguar detection in these arid landscapes. Here we develop a qPCR assay for the detection of jaguar mitochondrial DNA, show that large quantities of DNA (mean 66,820 copies/L) can be found in the drinking water of captive animals, and observe detectable levels of DNA (80 copies/L) in a wild habitat with known jaguar populations. We suggest that environmental DNA sampling may represent a useful, complementary sampling tool for detection of rare jaguars, although effective application would require careful consideration of DNA persistence time in the environment.
英文关键词	environmental DNA eDNA non-invasive genetics wildlife monitoring
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	SCI-E
WOS记录号	WOS:000654697900001
WOS关键词	ENVIRONMENTAL DNA METHODS
WOS类目	Ecology
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350283
作者单位	[Wilcox, Taylor M.; Dysthe, Joseph C.; Franklin, Thomas W.; Mason, Daniel H.; McKelvey, Kevin S.; Zarn, Katherine E.; Schwartz, Michael K.] US Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, USDA, Missoula, MT 59804 USA; [Caragiulo, Anthony] Amer Museum Nat Hist, Sackler Inst Comparat Genom, New York, NY 10024 USA
推荐引用方式 GB/T 7714	Wilcox, Taylor M.,Caragiulo, Anthony,Dysthe, Joseph C.,et al. Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water[J],2021,9.
APA	Wilcox, Taylor M..,Caragiulo, Anthony.,Dysthe, Joseph C..,Franklin, Thomas W..,Mason, Daniel H..,...&Schwartz, Michael K..(2021).Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water.FRONTIERS IN ECOLOGY AND EVOLUTION,9.
MLA	Wilcox, Taylor M.,et al."Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water".FRONTIERS IN ECOLOGY AND EVOLUTION 9(2021).