干旱区生态环境知识资源中心(Arid): Lipid Secretion by Parasitic Cells of Coccidioides Contributes to Disseminated Disease

Arid

DOI	10.3389/fcimb.2021.592826
	Lipid Secretion by Parasitic Cells of Coccidioides Contributes to Disseminated Disease
	Pelaez-Jaramillo, Carlos Alberto; Jimenez-Alzate, Maria Del Pilar; Araque-Marin, Pedronel; Hung, Chiung-Yu; Castro-Lopez, Natalia; Cole, Garry T.
通讯作者	Jimenez-Alzate, MD (corresponding author), Univ Texas San Antonio, Biol Dept, San Antonio, TX 78249 USA. ; Jimenez-Alzate, MD (corresponding author), Univ Texas San Antonio, South Texas Ctr Emerging Infect Dis, San Antonio, TX 78249 USA. ; Jimenez-Alzate, MD (corresponding author), Fac Nat & Exact Sci, Grp Interdisciplinario Estudios Mol, Chem Inst, Medellin, Antioquia, Colombia.
来源期刊	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN	2235-2988
出版年	2021
卷号	11
英文摘要	Coccidioides is a soil-borne fungal pathogen and causative agent of a human respiratory disease (coccidioidomycosis) endemic to semi-desert regions of southwestern United States, Mexico, Central and South America. Aerosolized arthroconidia inhaled by the mammalian host first undergo conversion to large parasitic cells (spherules, 80-100 mu m diameter) followed by endosporulation, a process by which the contents of spherules give rise to multiple endospores. The latter are released upon rupture of the maternal spherules and establish new foci of lung infection. A novel feature of spherule maturation prior to endosporulation is the secretion of a lipid-rich, membranous cell surface layer shed in vivo during growth of the parasitic cells and secretion into liquid culture medium during in vitro growth. Chemical analysis of the culture derived spherule outer wall (SOW) fraction showed that it is composed largely of phospholipids and is enriched with saturated fatty acids, including myristic, palmitic, elaidic, oleic, and stearic acid. NMR revealed the presence of monosaccharide- and disaccharide-linked acylglycerols and sphingolipids. The major sphingolipid components are sphingosine and ceramide. Primary neutrophils derived from healthy C57BL/6 and DBA/2 mice incubated with SOW lipids revealed a significant reduction in fungicidal activity against viable Coccidioides arthroconidia compared to incubation of neutrophils with arthroconidia alone. Host cell exposure to SOW lipids had no effect on neutrophil viability. Furthermore, C57BL/6 mice that were challenged subcutaneously with Coccidioides arthroconidia in the presence of the isolated SOW fraction developed disseminated disease, while control mice challenged with arthroconidia alone by the same route showed no dissemination of infection. We hypothesize that SOW lipids contribute to suppression of inflammatory response to Coccidioides infection. Studies are underway to characterize the immunosuppressive mechanism(s) of SOW lipids.
英文关键词	Coccidioides spherule outer wall phospholipids sphingolipids immunomodulation fungal lipids SOW-lipid extract
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000654735900001
WOS关键词	PARACOCCIDIOIDES-BRASILIENSIS ; GLUCOSYLCERAMIDE SYNTHASE ; MONOCLONAL-ANTIBODY ; METHYL-ESTERS ; IMMITIS ; VIRULENCE ; PATHOGENICITY ; MICE ; COMPONENTS ; EXPRESSION
WOS类目	Immunology ; Microbiology
WOS研究方向	Immunology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350270
作者单位	[Pelaez-Jaramillo, Carlos Alberto; Jimenez-Alzate, Maria Del Pilar; Hung, Chiung-Yu; Castro-Lopez, Natalia; Cole, Garry T.] Univ Texas San Antonio, Biol Dept, San Antonio, TX 78249 USA; [Pelaez-Jaramillo, Carlos Alberto; Jimenez-Alzate, Maria Del Pilar; Hung, Chiung-Yu; Castro-Lopez, Natalia; Cole, Garry T.] Univ Texas San Antonio, South Texas Ctr Emerging Infect Dis, San Antonio, TX 78249 USA; [Pelaez-Jaramillo, Carlos Alberto; Jimenez-Alzate, Maria Del Pilar] Fac Nat & Exact Sci, Grp Interdisciplinario Estudios Mol, Chem Inst, Medellin, Antioquia, Colombia; [Pelaez-Jaramillo, Carlos Alberto] Univ Antioquia, Sch Med, Microbiol & Parasitol Dept, Grp Micol Med, Medellin, Antioquia, Colombia; [Araque-Marin, Pedronel] Univ Escuela Ingn Antioquia, EIA Univ, Sch Life Sci, Envigado, Antioquia, Colombia
推荐引用方式 GB/T 7714	Pelaez-Jaramillo, Carlos Alberto,Jimenez-Alzate, Maria Del Pilar,Araque-Marin, Pedronel,et al. Lipid Secretion by Parasitic Cells of Coccidioides Contributes to Disseminated Disease[J],2021,11.
APA	Pelaez-Jaramillo, Carlos Alberto,Jimenez-Alzate, Maria Del Pilar,Araque-Marin, Pedronel,Hung, Chiung-Yu,Castro-Lopez, Natalia,&Cole, Garry T..(2021).Lipid Secretion by Parasitic Cells of Coccidioides Contributes to Disseminated Disease.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,11.
MLA	Pelaez-Jaramillo, Carlos Alberto,et al."Lipid Secretion by Parasitic Cells of Coccidioides Contributes to Disseminated Disease".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 11(2021).