干旱区生态环境知识资源中心(Arid): The Microbiome of Size-Fractionated Airborne Particles from the Sahara Region

Arid

DOI	10.1021/acs.est.0c06332
	The Microbiome of Size-Fractionated Airborne Particles from the Sahara Region
	Stern, Rebecca A.; Mahmoudi, Nagissa; Buckee, Caroline O.; Schartup, Amina T.; Koutrakis, Petros; Ferguson, Stephen T.; Wolfson, Jack M.; Wofsy, Steven C.; Daube, Bruce C.; Sunderland, Elsie M.
通讯作者	Stern, RA (corresponding author), Harvard Univ, Harvard John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA.
来源期刊	ENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN	0013-936X
EISSN	1520-5851
出版年	2021
卷号	55 期号:3 页码:1487-1496
英文摘要	Diverse airborne microbes affect human health and biodiversity, and the Sahara region of West Africa is a globally important source region for atmospheric dust. We collected size-fractionated (>10, 10-2.5, 2.5-1.0, 1.0-0.5, and <0.5 mu m) atmospheric particles in Mali, West Africa and conducted the first cultivation-independent study of airborne microbes in this region using 16S rRNA gene sequencing. Abundant and diverse microbes were detected in all particle size fractions at levels higher than those previously hypothesized for desert regions. Average daily abundance was 1.94 x 10(5) 16S rRNA copies/m(3). Daily patterns in abundance for particles <0.5 mu m differed significantly from other size fractions likely because they form mainly in the atmosphere and have limited surface resuspension. Particles >10 mu m contained the greatest fraction of daily abundance (51-62%) and had significantly greater diversity than smaller particles. Greater bacterial abundance of particles >2.5 mu m that are bigger than the average bacterium suggests that most airborne bacteria are present as aggregates or attached to particles rather than as free-floating cells. Particles >10 mu m have very short atmospheric lifetimes and thus tend to have more localized origins. We confirmed the presence of several potential pathogens using polymerase chain reaction that are candidates for viability and strain testing in future studies. These species were detected on all particle sizes tested, including particles <2.5 mu m that are expected to undergo long-range transport. Overall, our results suggest that the composition and sources of airborne microbes can be better discriminated by collecting size-fractionated samples.
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000618083600012
WOS关键词	AFRICAN DUST EVENT ; BACTERIAL COMMUNITIES ; GLOBAL ATMOSPHERE ; TRANSPORT ; IMPACT ; MICROORGANISMS ; VARIABILITY ; GREENGENES ; SOIL ; AIR
WOS类目	Engineering, Environmental ; Environmental Sciences
WOS研究方向	Engineering ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350159
作者单位	[Mahmoudi, Nagissa; Wofsy, Steven C.] McGill Univ, Dept Earth & Planetary Sci, Montreal, PQ H3A 0E8, Canada; [Buckee, Caroline O.] Harvard TH Chan Sch Publ Hlth, Ctr Communicable Dis Dynam, Dept Epidemiol, Boston, MA 02115 USA; [Schartup, Amina T.; Koutrakis, Petros; Ferguson, Stephen T.; Wolfson, Jack M.; Daube, Bruce C.; Sunderland, Elsie M.] Harvard TH Chan Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02115 USA; [Schartup, Amina T.] Scripps Inst Oceanog, La Jolla, CA 92037 USA; [Wofsy, Steven C.] Harvard Univ, Dept Earth & Planetary Sci, Cambridge, MA 02138 USA; [Stern, Rebecca A.; Sunderland, Elsie M.] Harvard Univ, Harvard John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA
推荐引用方式 GB/T 7714	Stern, Rebecca A.,Mahmoudi, Nagissa,Buckee, Caroline O.,et al. The Microbiome of Size-Fractionated Airborne Particles from the Sahara Region[J],2021,55(3):1487-1496.
APA	Stern, Rebecca A..,Mahmoudi, Nagissa.,Buckee, Caroline O..,Schartup, Amina T..,Koutrakis, Petros.,...&Sunderland, Elsie M..(2021).The Microbiome of Size-Fractionated Airborne Particles from the Sahara Region.ENVIRONMENTAL SCIENCE & TECHNOLOGY,55(3),1487-1496.
MLA	Stern, Rebecca A.,et al."The Microbiome of Size-Fractionated Airborne Particles from the Sahara Region".ENVIRONMENTAL SCIENCE & TECHNOLOGY 55.3(2021):1487-1496.