干旱区生态环境知识资源中心(Arid): Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species

Arid

DOI	10.1016/j.envexpbot.2021.104514
	Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species
	Visscher, Anne M.; Frances, Alba Latorre; Yeo, Maggie; Yan, Jie; Colville, Louise; Barreiro, Pablo Gomez; Pritchard, Hugh W.
通讯作者	Visscher, AM (corresponding author), Royal Bot Gardens, Dept Comparat Plant & Fungal Biol, Wakehurst Pl, Ardingly RH17 6TN, W Sussex, England.
来源期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
EISSN	1873-7307
出版年	2021
卷号	188
英文摘要	Extreme dry seed thermotolerance is an important trait in the context of plant adaptation to desert environments, yet the mechanisms underlying interspecific differences in seed extremophily remain little understood. Seeds of a selection of desert Cactaceae species (Leucostele litoralis, Leucostele skottsbergii, Eulychnia breviflora, Eriosyce paucicostata and Ferocactus wislizeni) were subjected to comparative biophysical and molecular analyses prior to (mature dry seeds) and post (imbibing seeds) heat treatment (103 degrees C). Biophysical properties of mature dry seeds, such as seed coat hardness and thermal transitions, were characterized through puncture force resistance and differential scanning calorimetry, respectively. In addition, the protein composition of dry seeds was compared by 2-D electrophoresis and de novo sequencing. Finally, oxidative DNA damage repair and gene expression during seed imbibition were analysed via ELISA (8-oxo-dG levels) and comparative transcriptomics. Results revealed that enhanced dry seed thermotolerance could not be explained by seed coat hardness, seed thermal transitions or (ROS-induced oxidative) DNA damage repair. Comparative proteomic analysis showed that the majority of overlapping proteins were more abundant in relatively heat sensitive species, suggesting that higher basal levels of (stress) proteins do not underly improved heat resilience in Cactaceae. However, comparative transcriptomics during imbibition following heat stress detected consistent expression patterns of heat- and drying-responsive genes that correlated with dry seed thermotolerance. These patterns included upregulation of positive (HSP101/CLPB1, MGE2, MBF1c, HSFB2A, RPN1a, RPT2a, DEGP14/PARK13, XPO1A, MAIGO2/MAG2, CAMTA1, ADH1) and downregulation of negative (RCF3, VOZ1, FAD7, ZAT12, RNP1) heat and drought tolerance regulators. Thus, our comparative analyses of extremophile Cactaceae species suggest that higher dry seed thermotolerance is realised through enhanced recovery from heat stress during imbibition, which is a critical stage in the plant life cycle.
英文关键词	Cactaceae Imbibition Proteome Seed germination Thermotolerance Transcriptome
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000662575500006
WOS关键词	HEAT-SHOCK PROTEINS ; MEDICAGO-TRUNCATULA SEEDS ; DNA-REPAIR ; PLANT TOLERANCE ; ABIOTIC STRESS ; ARABIDOPSIS-THALIANA ; DROUGHT TOLERANCE ; STABLE PROTEOME ; GENE-EXPRESSION ; REACTIVE OXYGEN
WOS类目	Plant Sciences ; Environmental Sciences
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350092
作者单位	[Visscher, Anne M.; Frances, Alba Latorre; Yeo, Maggie; Yan, Jie; Colville, Louise; Barreiro, Pablo Gomez; Pritchard, Hugh W.] Royal Bot Gardens, Dept Comparat Plant & Fungal Biol, Wakehurst Pl, Ardingly RH17 6TN, W Sussex, England; [Yan, Jie] Chengdu Univ Tradit Chinese Med, State Key Lab Characterist Chinese Med Resources, Chengdu 611137, Peoples R China
推荐引用方式 GB/T 7714	Visscher, Anne M.,Frances, Alba Latorre,Yeo, Maggie,et al. Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species[J],2021,188.
APA	Visscher, Anne M..,Frances, Alba Latorre.,Yeo, Maggie.,Yan, Jie.,Colville, Louise.,...&Pritchard, Hugh W..(2021).Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,188.
MLA	Visscher, Anne M.,et al."Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 188(2021).