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DOI10.1016/j.envexpbot.2021.104514
Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species
Visscher, Anne M.; Frances, Alba Latorre; Yeo, Maggie; Yan, Jie; Colville, Louise; Barreiro, Pablo Gomez; Pritchard, Hugh W.
通讯作者Visscher, AM (corresponding author), Royal Bot Gardens, Dept Comparat Plant & Fungal Biol, Wakehurst Pl, Ardingly RH17 6TN, W Sussex, England.
来源期刊ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN0098-8472
EISSN1873-7307
出版年2021
卷号188
英文摘要Extreme dry seed thermotolerance is an important trait in the context of plant adaptation to desert environments, yet the mechanisms underlying interspecific differences in seed extremophily remain little understood. Seeds of a selection of desert Cactaceae species (Leucostele litoralis, Leucostele skottsbergii, Eulychnia breviflora, Eriosyce paucicostata and Ferocactus wislizeni) were subjected to comparative biophysical and molecular analyses prior to (mature dry seeds) and post (imbibing seeds) heat treatment (103 degrees C). Biophysical properties of mature dry seeds, such as seed coat hardness and thermal transitions, were characterized through puncture force resistance and differential scanning calorimetry, respectively. In addition, the protein composition of dry seeds was compared by 2-D electrophoresis and de novo sequencing. Finally, oxidative DNA damage repair and gene expression during seed imbibition were analysed via ELISA (8-oxo-dG levels) and comparative transcriptomics. Results revealed that enhanced dry seed thermotolerance could not be explained by seed coat hardness, seed thermal transitions or (ROS-induced oxidative) DNA damage repair. Comparative proteomic analysis showed that the majority of overlapping proteins were more abundant in relatively heat sensitive species, suggesting that higher basal levels of (stress) proteins do not underly improved heat resilience in Cactaceae. However, comparative transcriptomics during imbibition following heat stress detected consistent expression patterns of heat- and drying-responsive genes that correlated with dry seed thermotolerance. These patterns included upregulation of positive (HSP101/CLPB1, MGE2, MBF1c, HSFB2A, RPN1a, RPT2a, DEGP14/PARK13, XPO1A, MAIGO2/MAG2, CAMTA1, ADH1) and downregulation of negative (RCF3, VOZ1, FAD7, ZAT12, RNP1) heat and drought tolerance regulators. Thus, our comparative analyses of extremophile Cactaceae species suggest that higher dry seed thermotolerance is realised through enhanced recovery from heat stress during imbibition, which is a critical stage in the plant life cycle.
英文关键词Cactaceae Imbibition Proteome Seed germination Thermotolerance Transcriptome
类型Article
语种英语
收录类别SCI-E
WOS记录号WOS:000662575500006
WOS关键词HEAT-SHOCK PROTEINS ; MEDICAGO-TRUNCATULA SEEDS ; DNA-REPAIR ; PLANT TOLERANCE ; ABIOTIC STRESS ; ARABIDOPSIS-THALIANA ; DROUGHT TOLERANCE ; STABLE PROTEOME ; GENE-EXPRESSION ; REACTIVE OXYGEN
WOS类目Plant Sciences ; Environmental Sciences
WOS研究方向Plant Sciences ; Environmental Sciences & Ecology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/350092
作者单位[Visscher, Anne M.; Frances, Alba Latorre; Yeo, Maggie; Yan, Jie; Colville, Louise; Barreiro, Pablo Gomez; Pritchard, Hugh W.] Royal Bot Gardens, Dept Comparat Plant & Fungal Biol, Wakehurst Pl, Ardingly RH17 6TN, W Sussex, England; [Yan, Jie] Chengdu Univ Tradit Chinese Med, State Key Lab Characterist Chinese Med Resources, Chengdu 611137, Peoples R China
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GB/T 7714
Visscher, Anne M.,Frances, Alba Latorre,Yeo, Maggie,et al. Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species[J],2021,188.
APA Visscher, Anne M..,Frances, Alba Latorre.,Yeo, Maggie.,Yan, Jie.,Colville, Louise.,...&Pritchard, Hugh W..(2021).Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,188.
MLA Visscher, Anne M.,et al."Comparative analyses of extreme dry seed thermotolerance in five Cactaceae species".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 188(2021).
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