干旱区生态环境知识资源中心(Arid): Isolation of Iranian Bacillus thuringiensis strains and characterization of lepidopteran-active cry genes

Arid

DOI	10.1186/s41938-021-00432-3
	Isolation of Iranian Bacillus thuringiensis strains and characterization of lepidopteran-active cry genes
	Rashki, Maryam; Maleki, Mahmood; Torkzadeh-Mahani, Masoud; Shakeri, Shahryar; Nezhad, Pariya Soltani
通讯作者	Rashki, M (corresponding author), Grad Univ Adv Technol, Inst Sci & High Technol & Environm Sci, Dept Biodivers, Kerman, Iran.
来源期刊	EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
ISSN	1110-1768
EISSN	2536-9342
出版年	2021
卷号	31 期号:1
英文摘要	Background Insecticidal crystal proteins (encoded by cry genes) produced by Bacillus thuringiensis (Bt) are fatal for insects of different orders such as Lepidoptera. The genes that encoded these crystal proteins can be detected on plasmids and chromosomal DNA and show different types in various strains. Therefore, the objective of this study was to determine molecular characteristics of Iranian Bt strains as well as their toxicity against Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae). Results The collection sites included fields, gardens, and desert and semi-desert areas in 8 provinces of Iran. For crystal formation, each isolate was cultured in T3 medium. The results showed that the Bt isolates produced different types of crystals including spherical (73.33%), bipyramidal (53.33%), irregular (40%), cubical (33.33%), and elliptical (13.33%). Plasmid DNA extraction was performed and showed that most of the strains exhibited similar pattern in the number and the size of the plasmid bands to those of Bt kurstaki (Btk). Some specific primers were used for PCR amplification to distinguish different crystal genes including cry1 (A, C, and D) and cry2A (a and b). The primers related to cry1D and cry2Aa1 genes produced no amplicons. The results revealed that the most abundant gene was cry1-type. All strains analyzed for the cry2Ab2 gene presented unexpected bands. Electrophoretic profile of the protein crystals showed bands with different diversity in number, and size ranged from about 16 to 140 KDa. The bioassay result of some more toxic strains exhibited that the pathogenicity of 1019 was higher than the rest, even the reference strain, Btk. However, the toxicity of other strains was the same as Btk. According to the phylogenetic tree, 1019 was located in the same group with Bt subspecies coreanensis, Bt subspecies indiana and Bt subspecies tolworthi. Conclusions The investigated Iranian strains had the lepidopteran-active cry genes. The strains with the same toxicity to E. kuehniella had various cry genes, plasmid, and crystal protein profiles and vice versa. Therefore, characterization of cry genes of native strains could lead to access potent isolates as biocontrol agents against native insect pests.
英文关键词	Bacteria Bioassay Cry gene Crystal protein Iranian isolates Polymerase chain reaction Plasmid DNA
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000657738400002
WOS关键词	CRYSTAL ; PROTEINS ; DIVERSITY ; TOXINS
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350058
作者单位	[Rashki, Maryam] Grad Univ Adv Technol, Inst Sci & High Technol & Environm Sci, Dept Biodivers, Kerman, Iran; [Maleki, Mahmood; Torkzadeh-Mahani, Masoud; Shakeri, Shahryar] Grad Univ Adv Technol, Inst Sci & High Technol & Environm Sci, Dept Biotechnol, Kerman, Iran; [Nezhad, Pariya Soltani] Urmia Univ, Dept Plant Protect, Coll Agr, Orumiyeh, Iran
推荐引用方式 GB/T 7714	Rashki, Maryam,Maleki, Mahmood,Torkzadeh-Mahani, Masoud,et al. Isolation of Iranian Bacillus thuringiensis strains and characterization of lepidopteran-active cry genes[J],2021,31(1).
APA	Rashki, Maryam,Maleki, Mahmood,Torkzadeh-Mahani, Masoud,Shakeri, Shahryar,&Nezhad, Pariya Soltani.(2021).Isolation of Iranian Bacillus thuringiensis strains and characterization of lepidopteran-active cry genes.EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL,31(1).
MLA	Rashki, Maryam,et al."Isolation of Iranian Bacillus thuringiensis strains and characterization of lepidopteran-active cry genes".EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL 31.1(2021).