干旱区生态环境知识资源中心(Arid): Short-term perfluorooctane sulfonate exposure impairs Leydig cell regeneration in the adult rat testis via targeting hedgehog signaling

Arid

DOI	10.1016/j.ecoenv.2021.112121
	Short-term perfluorooctane sulfonate exposure impairs Leydig cell regeneration in the adult rat testis via targeting hedgehog signaling
	Mao, Baiping; Li, Chao; Wen, Zina; Li, Huitao; Wang, Yiyan; Chen, Lanlan; Lian, Qingquan; Ge, Ren-Shan
通讯作者	Lian, QQ ; Ge, RS (corresponding author), Wenzhou Med Univ, Dept Anesthesiol, Affiliated Hosp 2, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China.
来源期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
EISSN	1090-2414
出版年	2021
卷号	214
英文摘要	Perfluorooctane sulfonate is related to male reproductive dysfunction in rats and humans. However, the underlying mechanism remains unknown. He re, we reported the effects of short-term exposure to perfluorooctane sulfonate on the regeneration of Leydig cells in vivo and investigated possible mechanisms in vitro. After adult male Sprague-Dawley rats were gavaged perfluorooctane sulfonate (0, 5 or 10 mg/kg/day) for 7 days and then injected intraperitoneally ethane dimethane sulfonate next day to eliminate Leydig cells, the Leydig cell regeneration process was monitored. Perfluorooctane sulfonate significantly lowered serum testosterone levels, reduced the number of regenerated Leydig cells, down-regulated the expression of Lhcgr, Scarb1, Star, Cyp11a1, Hsd3b1, Cyp17a1, and Dhh) and their proteins at doses of 5 and 10 mg/kg 35 and 56 days after ethane dimethane sulfonate. Using a 3D seminiferous tubule culture system to study the development of stem Leydig cells, we found that perfluorooctane sulfonate inhibited stem Leydig cell proliferation and differentiation and hedgehog signaling pathway. In conclusion, a short-term exposure to perfluorooctane sulfonate can inhibit the development of stem Leydig cells into the Leydig cell lineage via direct suppression of hedgehog signaling pathway and indirect inhibition of desert hedgehog section by Sertoli cells.
英文关键词	Leydig cells Leydig cell regeneration Perfluorooctane sulfonate Stem cells Steroidogenesis Hedgehog signaling
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000634521400003
WOS类目	Environmental Sciences ; Toxicology
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/350056
作者单位	[Mao, Baiping; Li, Huitao; Wang, Yiyan; Chen, Lanlan; Lian, Qingquan; Ge, Ren-Shan] Wenzhou Med Univ, Dept Anesthesiol, Affiliated Hosp 2, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China; [Mao, Baiping; Li, Chao; Li, Huitao; Wang, Yiyan; Chen, Lanlan; Lian, Qingquan; Ge, Ren-Shan] Wenzhou Med Univ, Yuying Childrens Hosp, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China; [Li, Chao] Wenzhou Med Univ, Ctr Sci Res, Affiliated Hosp 2, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China; [Wen, Zina] Xinan Gynecol Hosp, Chengdu, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Mao, Baiping,Li, Chao,Wen, Zina,et al. Short-term perfluorooctane sulfonate exposure impairs Leydig cell regeneration in the adult rat testis via targeting hedgehog signaling[J],2021,214.
APA	Mao, Baiping.,Li, Chao.,Wen, Zina.,Li, Huitao.,Wang, Yiyan.,...&Ge, Ren-Shan.(2021).Short-term perfluorooctane sulfonate exposure impairs Leydig cell regeneration in the adult rat testis via targeting hedgehog signaling.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,214.
MLA	Mao, Baiping,et al."Short-term perfluorooctane sulfonate exposure impairs Leydig cell regeneration in the adult rat testis via targeting hedgehog signaling".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 214(2021).