干旱区生态环境知识资源中心(Arid): Geomorphologic study of human tooth root surfaces following simulated avulsion by scanning electron microscopy

Arid

DOI	10.1111/edt.12642
	Geomorphologic study of human tooth root surfaces following simulated avulsion by scanning electron microscopy
	Ding, Qian-Wen; Lin, Mei; Zhang, Xin; Gong, Yi
通讯作者	Ding, QW (corresponding author), Capital Med Univ, Sch Stomatol, 4th Tian Tan Xi Li, Beijing 100050, Peoples R China. ; Ding, QW (corresponding author), Capital Med Univ, Beijing Stomatol Hosp, 4th Tian Tan Xi Li, Beijing 100050, Peoples R China.
来源期刊	DENTAL TRAUMATOLOGY
ISSN	1600-4469
EISSN	1600-9657
出版年	2021
卷号	37 期号:3 页码:457-463
英文摘要	Background/Aims Tooth avulsion is the most severe traumatic tooth injury. Immediate replantation after avulsion provides the highest success rate, whereas delayed replantation with dehydration for >1 h always results in ankylosis, replacement resorption, and eventual tooth loss. The aim of this study was to investigate the geomorphologic change of ultrastructure on tooth root surfaces due to dehydration. Material and Methods Twenty-four sound human premolars and fully developed third molars without periodontitis were selected for the experiment. Roots were separated into 6-7 pieces with an intact root surface area (3 x 3 mm(2)) and then divided into the following groups: fresh group, prolonged dehydration groups (air drying for 1, 2, 4, 12, or 24 h), and dehydrated teeth (air drying for 2 h) treated with acid (Tris-HCl buffer or citric acid buffer). More than six pieces of root from each group were subjected to scanning electron microscopy (SEM) observation. Captured images were exported to ImageJ software to quantitatively analyze the areas covered with fibers. Statistical significance was determined by comparing the means of the different groups using t-testing or one-way analysis of variance followed by post hoc testing. Results Fibrous vegetation covering the cementum was observed on the fresh root surface by SEM. This was destroyed by dehydration (>1 h), resulting in a root surface resembling the Gobi Desert. The difference was statistically significant (p < .001). Root surface deteriorated by dehydration could be recovered by the re-use of the fibers embedded in the cementum as acid demineralization of the outer layer of cementum exposed the embedded fibers to simulate the geomorphology of fresh root surface. Conclusions Dehydrated teeth had deteriorated geomorphology of the root surface, which could be reversed by the re-use of the fibers embedded in the cementum using citric acid. Direct evidence from SEM gives new insights into the replantation of dehydrated avulsed teeth.
英文关键词	dehydration delayed replantation periodontal ligament fiber scanning electron microscopy tooth avulsion
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000601917500001
WOS关键词	HEALING-PROCESS ; CITRIC-ACID ; STEM-CELLS ; REPLANTATION ; TEETH ; FLUORIDE ; STORAGE ; MATRIX
WOS类目	Dentistry, Oral Surgery & Medicine
WOS研究方向	Dentistry, Oral Surgery & Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349929
作者单位	[Ding, Qian-Wen; Zhang, Xin; Gong, Yi] Capital Med Univ, Beijing Stomatol Hosp, Emergency Dept, Beijing, Peoples R China; [Ding, Qian-Wen; Zhang, Xin; Gong, Yi] Capital Med Univ, Sch Stomatol, 4th Tian Tan Xi Li, Beijing 100050, Peoples R China; [Lin, Mei] Capital Med Univ, Beijing Chao Yan Hosp, Dept Stomatol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ding, Qian-Wen,Lin, Mei,Zhang, Xin,et al. Geomorphologic study of human tooth root surfaces following simulated avulsion by scanning electron microscopy[J],2021,37(3):457-463.
APA	Ding, Qian-Wen,Lin, Mei,Zhang, Xin,&Gong, Yi.(2021).Geomorphologic study of human tooth root surfaces following simulated avulsion by scanning electron microscopy.DENTAL TRAUMATOLOGY,37(3),457-463.
MLA	Ding, Qian-Wen,et al."Geomorphologic study of human tooth root surfaces following simulated avulsion by scanning electron microscopy".DENTAL TRAUMATOLOGY 37.3(2021):457-463.