干旱区生态环境知识资源中心(Arid): Determination of Polybrominated Diphenyl Ethers and Hydroxylated Analogues in Serum Using High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry with Online Solid Phase Extraction

Arid

DOI	10.1016/S1872-2040(21)60091-9
	Determination of Polybrominated Diphenyl Ethers and Hydroxylated Analogues in Serum Using High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry with Online Solid Phase Extraction
	Jin Jin; Song Shan-Junz; Peng Zi-Juan; Guo Feng; Li Peng-Hui
通讯作者	Song, SJ (corresponding author), Natl Inst Metrol, Beijing 100029, Peoples R China. ; Guo, F (corresponding author), Natl Res Ctr Geoanal, Beijing 100037, Peoples R China.
来源期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
EISSN	1872-2040
出版年	2021
卷号	49 期号:4 页码:520-526
英文摘要	Online solid phase extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry (Online SPE-HPLC-ICP-MS) technique was developed for simultaneous determination of 6 kinds of polybrominated diphenyl ethers and 5 kinds of hydroxyl metabolites in serum. A serum sample (200 mu L) was directly analyzed after protein precipitation and dilution. The target analytes were automatically enriched on a solid phase extraction column, and then separated and analyzed by liquid chromatography-inductively coupled plasma mass spectrometry with methanol/acetonitrile/water as mobile phase. The main parameters such as loading rate, elution time and elution solvent were optimized. As a result, Oasis HLB was selected as the SPE column, the loading solvent was pure water at a rate of 3.0 mL/min, the elution solvent was methanol-acetonitrile-water (54:26:20, V/V), the elution flow rate was 1.0 mL/min, and the liquid chromatography column was ZORBAX Eclipse Plus C18. The limits of quantification (LOQ) of the method were 0.14-0.74 ng/L, the recoveries were 61.8%-77.7%, and the relative standard deviations (RSDs) of the results were 3.7%-10.1% (n = 3). The established method was successfully applied to the analysis of 30 serum samples. A total of 4 kinds of polybrominated diphenyl ethers and 2 kinds of hydroxy polybrominated diphenyl ethers were detected, and their occurrence characteristics were consistent with previous reports. The results showed that this method could be applied to analyze actual samples in large quantities, which was of great practical value.
英文关键词	Online solid phase extraction High performance liquid chromatography Inductively coupled plasma mass spectrometry Serum Polybrominated diphenyl ethers Hydroxy polybrominated diphenyl ethers
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000640209500007
WOS关键词	ENVIRONMENTAL WATER SAMPLES ; OH-PBDES ; METABOLITES ; CHINA
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349847
作者单位	[Jin Jin; Li Peng-Hui] Tianjin Univ Technol, Tianjin 300384, Peoples R China; [Jin Jin; Song Shan-Junz; Peng Zi-Juan] Natl Inst Metrol, Beijing 100029, Peoples R China; [Guo Feng] Natl Res Ctr Geoanal, Beijing 100037, Peoples R China
推荐引用方式 GB/T 7714	Jin Jin,Song Shan-Junz,Peng Zi-Juan,et al. Determination of Polybrominated Diphenyl Ethers and Hydroxylated Analogues in Serum Using High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry with Online Solid Phase Extraction[J],2021,49(4):520-526.
APA	Jin Jin,Song Shan-Junz,Peng Zi-Juan,Guo Feng,&Li Peng-Hui.(2021).Determination of Polybrominated Diphenyl Ethers and Hydroxylated Analogues in Serum Using High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry with Online Solid Phase Extraction.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,49(4),520-526.
MLA	Jin Jin,et al."Determination of Polybrominated Diphenyl Ethers and Hydroxylated Analogues in Serum Using High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry with Online Solid Phase Extraction".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49.4(2021):520-526.