干旱区生态环境知识资源中心(Arid): Optimization of microcystin biodegradation by bacterial community YFMCD4 using response surface method

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DOI	10.1016/j.chemosphere.2021.129897
	Optimization of microcystin biodegradation by bacterial community YFMCD4 using response surface method
	Guo, Jian; Wei, Jia; Huang, Feiyu; Massey, Isaac Yaw; Luo, Jiayou; Yang, Fei
通讯作者	Yang, F (corresponding author), Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.
来源期刊	CHEMOSPHERE
ISSN	0045-6535
EISSN	1879-1298
出版年	2021
卷号	274
英文摘要	The increasing production of microcystin-LR (MC-LR) causing animal and human health issues is found in eutrophic water bodies, marine habitats and desert environments. The health threat posed by MC-LR has led to the establishment of World Health Organization's water guideline value of 1 mu g/mL. Combating this has increased the search for cost-effective approach to degrade MC-LR. The study aimed to optimize the MC-degrading environmental factors of bacterial community YFMCD4. Response surface methodology (RSM) was employed to evaluate the influence of varying temperatures, pH and initial MC-LR concentration on the biodegradation efficiency of MC-LR by bacterial community YFMCD4. The optimal MC-LR biodegradation environmental factors were found to be 30 degrees C, pH 7 and 2 mu g/mL initial MC-LR. The biodegradation rate reached 100% after 10 h. YFMCD4 mainly consisted of genera Alacligenes, Sphingobacterium and Pseudomonas using High-throughput pyrosequencing technology. The mlrA gene encoding MlrA enzyme considered most important for MC-LR biodegradation was obtained from YFMCD4. Data demonstrated that the bacterial structure and biodegradation efficiency of YFMCD4 varied with the change of environmental factors including temperature, pH and MC-LR concentrations. RSM is considered a good method to examine the optimal biodegradation environmental conditions for MC-LR. To date, RSM and High-throughput pyrosequencing technology are employed to optimize the biodegradation conditions (30 degrees C, pH 7 and 2 mu g/mL initial MC-LR) and analyze the structure of bacterial community for the first time. (C) 2021 Elsevier Ltd. All rights reserved.
英文关键词	Microcystin-LR biodegradation Bacterial community structure Response surface methodology mlrA
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000642944800093
WOS关键词	HETEROLOGOUS EXPRESSION ; ENZYMATIC PATHWAY ; LR DEGRADATION ; RR ; REMOVAL ; GENE ; CYANOBACTERIA ; MLRA
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349838
作者单位	[Yang, Fei] Univ South China, Sch Publ Hlth, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China; [Guo, Jian] Cent South Univ, Xiangya Stomatol Hosp, Changsha 410008, Hunan, Peoples R China; [Guo, Jian] Cent South Univ, Xiangya Sch Stomatol, Changsha 410008, Hunan, Peoples R China; [Wei, Jia; Huang, Feiyu; Massey, Isaac Yaw; Luo, Jiayou; Yang, Fei] Cent South Univ, Xiangya Sch Publ Hlth, Hunan Prov Key Lab Clin Epidemiol, Changsha 410078, Peoples R China
推荐引用方式 GB/T 7714	Guo, Jian,Wei, Jia,Huang, Feiyu,et al. Optimization of microcystin biodegradation by bacterial community YFMCD4 using response surface method[J],2021,274.
APA	Guo, Jian,Wei, Jia,Huang, Feiyu,Massey, Isaac Yaw,Luo, Jiayou,&Yang, Fei.(2021).Optimization of microcystin biodegradation by bacterial community YFMCD4 using response surface method.CHEMOSPHERE,274.
MLA	Guo, Jian,et al."Optimization of microcystin biodegradation by bacterial community YFMCD4 using response surface method".CHEMOSPHERE 274(2021).