干旱区生态环境知识资源中心(Arid): Biopolymer production by halotolerant bacteria isolated from Caatinga biome

Arid

DOI	10.1007/s42770-021-00426-1
	Biopolymer production by halotolerant bacteria isolated from Caatinga biome
	Parada-Pinilla, Maria Paula; Ferreira, Maria Alejandra; Roncallo, Juan Camilo; Santos, Suikinai Nobre; Melo, Itamar Soares; Assef, Alexia Nathalia Brigido; Wilke, Diego Veras; Silva, Luiziana F.; Garrido, Leandro Maza; Araujo, Welington Luiz; Padilla, Gabriel
通讯作者	Padilla, G (corresponding author), Univ Sao Paulo, Inst Biomed Sci, Bioprod Lab, Sao Paulo, Brazil.
来源期刊	BRAZILIAN JOURNAL OF MICROBIOLOGY
ISSN	1517-8382
EISSN	1678-4405
出版年	2021
卷号	52 期号:2 页码:547-559
英文摘要	Saline environments are extreme habitats with a high diversity of microorganisms source of a myriad of biomolecules. These microorganisms are assigned as extremophiles recognized to be producers of new natural compounds, which can be synthesized by helping to survive under harshness and extreme conditions. In Brazil, in the saline and semi-arid region of Areia Branca (Caatinga biome), halotolerant bacteria (able to growth at high NaCl concentrations) were isolated from rhizosphere of native plants Blutaparon portulacoides and Spergularia sp. and their biopolymer production was studied. A total of 25 bacterial isolates were identified at genus level based on 16S rRNA gene sequence analysis. Isolates were mainly Gram-positive bacteria from Bacillaceae, Staphylococcaceae, Microbacteriaceae, and Bacillales XII incertae sedis families, affiliates to Bacillus, Staphylococcus, Curtobacterium, and Exiguobacterium genera, respectively. One of the Gram-negative isolates was identified as member of the Pseudomonadaceae family, genus Pseudomonas. All the identified strains were halotolerant bacteria with optimum growth at 0.6-2.0 M salt concentrations. Assays for biopolymer production showed that the halotolerant strains are a rich source of compounds as polyhydroxyalkanoates (PHA), biodegradable biopolymer, such as poly(3-hydroxybutyrate) (PHB) produced from low-cost substrates, and exopolysaccharides (EPS), such as hyaluronic acid (HA), metabolite of great interest to the cosmetic and pharmaceutical industry. Also, eight bacterial EPS extracts showed immunostimulatory activity, promising results that can be used in biomedical applications. Overall, our findings demonstrate that these biomolecules can be produced in culture medium with 0.6-2.0 M NaCl concentrations, relevant feature to avoid costly production processes. This is the first report of biopolymer-producing bacteria from a saline region of Caatinga biome that showed important biological activities.
英文关键词	Biopolymers Halotolerant microorganisms Polyhydroxyalkanoates (PHA) Exopolysaccharides (EPS) Immunostimulatory activity Hyaluronic acid (HA) Caatinga biome
类型	Article
语种	英语
开放获取类型	DOAJ Gold
收录类别	SCI-E
WOS记录号	WOS:000610871200001
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349759
作者单位	[Parada-Pinilla, Maria Paula; Ferreira, Maria Alejandra; Roncallo, Juan Camilo; Silva, Luiziana F.; Garrido, Leandro Maza; Padilla, Gabriel] Univ Sao Paulo, Inst Biomed Sci, Bioprod Lab, Sao Paulo, Brazil; [Santos, Suikinai Nobre; Melo, Itamar Soares] Brazilian Agr Res Corp Embrapa, Environm Microbiol Lab, Sao Paulo, Brazil; [Assef, Alexia Nathalia Brigido; Wilke, Diego Veras] Univ Fed Ceara, Lab Bioprospecting & Marine Biotechnol, Fortaleza, Ceara, Brazil; [Araujo, Welington Luiz] Univ Sao Paulo, Inst Biomed Sci, Mol Biol & Microbial Ecol Lab, Sao Paulo, Brazil
推荐引用方式 GB/T 7714	Parada-Pinilla, Maria Paula,Ferreira, Maria Alejandra,Roncallo, Juan Camilo,et al. Biopolymer production by halotolerant bacteria isolated from Caatinga biome[J],2021,52(2):547-559.
APA	Parada-Pinilla, Maria Paula.,Ferreira, Maria Alejandra.,Roncallo, Juan Camilo.,Santos, Suikinai Nobre.,Melo, Itamar Soares.,...&Padilla, Gabriel.(2021).Biopolymer production by halotolerant bacteria isolated from Caatinga biome.BRAZILIAN JOURNAL OF MICROBIOLOGY,52(2),547-559.
MLA	Parada-Pinilla, Maria Paula,et al."Biopolymer production by halotolerant bacteria isolated from Caatinga biome".BRAZILIAN JOURNAL OF MICROBIOLOGY 52.2(2021):547-559.