干旱区生态环境知识资源中心(Arid): Dynamic transcriptome analysis identifies genes related to fatty acid biosynthesis in the seeds of Prunus pedunculata Pall

Arid

DOI	10.1186/s12870-021-02921-x
	Dynamic transcriptome analysis identifies genes related to fatty acid biosynthesis in the seeds of Prunus pedunculata Pall
	Bao, Wenquan; Ao, Dun; Wang, Lin; Ling, Zhihao; Chen, Maoshan; Bai, Yue; Wuyun, Ta-Na; Chen, Junxing; Zhang, Shuning; Li, Fengming
通讯作者	Wang, L (corresponding author), Chinese Acad Forestry, Nontimber Forest Res & Dev Ctr, State Key Lab Tree Genet & Breeding, Zhengzhou 450003, Peoples R China.
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2021
卷号	21 期号:1
英文摘要	Background: Prunus pedunculata Pall, the deciduous shrub of Amygdalus subgenus in Rosaceae, is a new kind of desert oil-bearing tree. It has a long story of being planted in the West and North of China for sand fixation and desert control. In addition, the seeds of P. pedunculata are rich of oil, especially the monounsaturated fatty acid and polyunsaturated fatty acid. However, little is known about the molecular mechanisms of oil accumulation during the seed development of P. pedunculata. Results: The seeds of P. pedunculata from three independent plants at 10, 18, 24, 31, 39, 45, 59 and 73 days after flowering (DAF) were obtained and the oil compositions were evaluated. It showed that oleic acid was the dominant type of oil content in the mature seeds (from 32.724% at 10DAF to 72.06% at 73DAF). Next, transcriptome sequencing for the developing seeds produced 988.795 million high quality reads and TRINITY assembled 326,271 genes for the first transcriptome for P. pedunculata. After the assembled transcriptome was evaluated by BUSCO with 85.9% completeness, we identified 195,342, 109,850 and 121,897 P. pedunculata genes aligned to NR, GO and KEGG pathway databases, respectively. Then, we predicted 23,229 likely proteins from the assembled transcriptome and identified 1917 signal peptides and 5512 transmembrane related proteins. In the developing seeds we detected 91,362 genes (average FPKM > 5) and correlation analysis indicated three possible development stages - early (10 - 24DAF), middle (31 - 45DAF) and late (59 73DAF). We next analyzed the differentially expressed genes (DEGs) in the developing seeds. Interestingly, compared to 10DAF the number of DEGs was increased from 4406 in 18DAF to 27,623 in 73DAF. Based on the gene annotation, we identified 753, 33, 8 and 645 DEGs related to the fatty acid biosynthesis, lipid biosynthesis, oil body and transcription factors. Notably, GPAT, DGD1, LACS2, UBC and RING were highly expressed at the early development stage, w6-FAD, SAD, ACP, ACCA and AHG1 were highly expressed at the middle development stage, and LACS6, DGD1, ACAT1, AGPAT, WSD1, EGY2 and oleosin genes were highly expressed at the late development stage. Conclusions: This is the first time to study the developing seed transcriptome of P. pedunculata and our findings will provide a valuable resource for future studies. More importantly, it will improve our understanding of molecular mechanisms of oil accumulation in P. pedunculata.
英文关键词	Fatty acid Oleic acid Oil accumulation Developing seeds Prunus pedunculata Transcriptome
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000634846200003
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349733
作者单位	[Bao, Wenquan; Ao, Dun; Bai, Yue; Chen, Junxing; Zhang, Shuning; Li, Fengming] Inner Mongolia Agr Univ, Hohhot 010018, Peoples R China; [Wang, Lin; Wuyun, Ta-Na] Chinese Acad Forestry, Nontimber Forest Res & Dev Ctr, State Key Lab Tree Genet & Breeding, Zhengzhou 450003, Peoples R China; [Ling, Zhihao] Chengdu Jiyu Technol, Chengdu 610213, Sichuan, Peoples R China; [Chen, Maoshan] Monash Univ, Australian Ctr Blood Dis, Cent Clin Sch, Melbourne, Vic 3004, Australia
推荐引用方式 GB/T 7714	Bao, Wenquan,Ao, Dun,Wang, Lin,et al. Dynamic transcriptome analysis identifies genes related to fatty acid biosynthesis in the seeds of Prunus pedunculata Pall[J],2021,21(1).
APA	Bao, Wenquan.,Ao, Dun.,Wang, Lin.,Ling, Zhihao.,Chen, Maoshan.,...&Li, Fengming.(2021).Dynamic transcriptome analysis identifies genes related to fatty acid biosynthesis in the seeds of Prunus pedunculata Pall.BMC PLANT BIOLOGY,21(1).
MLA	Bao, Wenquan,et al."Dynamic transcriptome analysis identifies genes related to fatty acid biosynthesis in the seeds of Prunus pedunculata Pall".BMC PLANT BIOLOGY 21.1(2021).