Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s00449-020-02507-1 |
Isolation, identification and bioactivity analysis of an endophytic fungus isolated from Aloe vera collected from Asir desert, Saudi Arabia | |
Ameen, Fuad; Stephenson, Steven L.; AlNadhari, Saleh; Yassin, Mohamed A. | |
通讯作者 | Ameen, F (corresponding author), King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia. |
来源期刊 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
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ISSN | 1615-7591 |
EISSN | 1615-7605 |
出版年 | 2021 |
卷号 | 44期号:6页码:1063-1070 |
英文摘要 | Endophytic fungi isolated from desert plants are among the less known organisms with potentially valuable applications. The bioactivities of an endophytic fungus isolated from Aloe vera, a plant found in central regions of Asir desert, Saudi Arabia. Based on primary phytochemical screening, an efficient isolate was selected and identified according to the sequence analysis of the internal spacer regions ITS1, ITS4 and the 5.8S region as Preussia africana belonging to the family Sporormiaceae. The crude extract of this fungus was evaluated for its bioactivities. Under static conditions, the crude extract at a concentration of 500 mu g/mL had a strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging rate of 87%, whereas a higher concentration (100 mu g/mL) had an astounding wound healing effect (42.6% at 48 h) when compared to positive control. Moreover, the crude extract with a concentration of 50 mu g/mL was active against almost all cancer cell lines such as HeLa (cervical cancer), Hep G2 (liver cancer), MCF-7 (breast cancer), A549 (lung cancer), LN-229 (glioblastoma), A-431 (skin cancer), and kidney cell line (HEK 293T). The results suggest that the endophytic fungus P. africana from A. vera has wide therapeutic applications against severe disease conditions. |
英文关键词 | Endophytic fungi Preussia africana Aloe vera Bioactivity DPPH Anticancer activity |
类型 | Article |
语种 | 英语 |
收录类别 | SCI-E |
WOS记录号 | WOS:000611439100001 |
WOS类目 | Biotechnology & Applied Microbiology ; Engineering, Chemical |
WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
来源机构 | King Saud University |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/349718 |
作者单位 | [Ameen, Fuad; Yassin, Mohamed A.] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia; [Stephenson, Steven L.] Univ Arkansas, Dept Biol Sci, Fayetteville, AR 72701 USA; [AlNadhari, Saleh] King Saud Univ, Coll Agr, Dept Plant Protect, Riyadh, Saudi Arabia; [Yassin, Mohamed A.] Plant Pathol Res Inst, Agr Res Ctr, Giza, Egypt |
推荐引用方式 GB/T 7714 | Ameen, Fuad,Stephenson, Steven L.,AlNadhari, Saleh,et al. Isolation, identification and bioactivity analysis of an endophytic fungus isolated from Aloe vera collected from Asir desert, Saudi Arabia[J]. King Saud University,2021,44(6):1063-1070. |
APA | Ameen, Fuad,Stephenson, Steven L.,AlNadhari, Saleh,&Yassin, Mohamed A..(2021).Isolation, identification and bioactivity analysis of an endophytic fungus isolated from Aloe vera collected from Asir desert, Saudi Arabia.BIOPROCESS AND BIOSYSTEMS ENGINEERING,44(6),1063-1070. |
MLA | Ameen, Fuad,et al."Isolation, identification and bioactivity analysis of an endophytic fungus isolated from Aloe vera collected from Asir desert, Saudi Arabia".BIOPROCESS AND BIOSYSTEMS ENGINEERING 44.6(2021):1063-1070. |
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