干旱区生态环境知识资源中心(Arid): Crucial Role of Juvenile Hormone Receptor Components Methoprene-Tolerant and Taiman in Sexual Maturation of Adult Male Desert Locusts

Arid

DOI	10.3390/biom11020244
	Crucial Role of Juvenile Hormone Receptor Components Methoprene-Tolerant and Taiman in Sexual Maturation of Adult Male Desert Locusts
	Holtof, Michiel; Van Lommel, Joachim; Gijbels, Marijke; Dekempeneer, Elfie; Nicolai, Bart; Vanden Broeck, Jozef; Marchal, Elisabeth
通讯作者	Vanden Broeck, J (corresponding author), Dept Biol, Mol Dev Physiol & Signal Transduct Lab, Div Anim Physiol & Neurobiol, Naamsestr 59,Box 2465, B-3000 Leuven, Belgium.
来源期刊	BIOMOLECULES
EISSN	2218-273X
出版年	2021
卷号	11 期号:2
英文摘要	Currently (2020), Africa and Asia are experiencing the worst desert locust (Schistocerca gregaria) plague in decades. Exceptionally high rainfall in different regions caused favorable environmental conditions for very successful reproduction and population growth. To better understand the molecular mechanisms responsible for this remarkable reproductive capacity, as well as to fill existing knowledge gaps regarding the regulation of male reproductive physiology, we investigated the role of methoprene-tolerant (Scg-Met) and Taiman (Scg-Tai), responsible for transducing the juvenile hormone (JH) signal, in adult male locusts. We demonstrated that knockdown of these components by RNA interference strongly inhibits male sexual maturation, severely disrupting reproduction. This was evidenced by the inability to show mating behavior, the absence of a yellow-colored cuticle, the reduction of relative testes weight, and the drastically reduced phenylacetonitrile (PAN) pheromone levels of the treated males. We also observed a reduced relative weight, as well as relative protein content, of the male accessory glands in Scg-Met knockdown locusts. Interestingly, in these animals the size of the corpora allata (CA), the endocrine glands where JH is synthesized, was significantly increased, as well as the transcript level of JH acid methyltransferase (JHAMT), a rate-limiting enzyme in the JH biosynthesis pathway. Moreover, other endocrine pathways appeared to be affected by the knockdown, as evidenced by changes in the expression levels of the insulin-related peptide and two neuroparsins in the fat body. Our results demonstrate that JH signaling pathway components play a crucial role in male reproductive physiology, illustrating their potential as molecular targets for pest control.
英文关键词	accessory gland corpora allata courtship insect Kruppel-homolog 1 pheromone testis
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000622149300001
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349717
作者单位	[Holtof, Michiel; Van Lommel, Joachim; Gijbels, Marijke; Vanden Broeck, Jozef; Marchal, Elisabeth] Dept Biol, Mol Dev Physiol & Signal Transduct Lab, Div Anim Physiol & Neurobiol, Naamsestr 59,Box 2465, B-3000 Leuven, Belgium; [Dekempeneer, Elfie; Nicolai, Bart] Katholieke Univ Leuven, Div Mechatron Biostat & Sensors MeBioS, Willem Croylaan 42,Box 2428, B-3001 Leuven, Belgium
推荐引用方式 GB/T 7714	Holtof, Michiel,Van Lommel, Joachim,Gijbels, Marijke,et al. Crucial Role of Juvenile Hormone Receptor Components Methoprene-Tolerant and Taiman in Sexual Maturation of Adult Male Desert Locusts[J],2021,11(2).
APA	Holtof, Michiel.,Van Lommel, Joachim.,Gijbels, Marijke.,Dekempeneer, Elfie.,Nicolai, Bart.,...&Marchal, Elisabeth.(2021).Crucial Role of Juvenile Hormone Receptor Components Methoprene-Tolerant and Taiman in Sexual Maturation of Adult Male Desert Locusts.BIOMOLECULES,11(2).
MLA	Holtof, Michiel,et al."Crucial Role of Juvenile Hormone Receptor Components Methoprene-Tolerant and Taiman in Sexual Maturation of Adult Male Desert Locusts".BIOMOLECULES 11.2(2021).