干旱区生态环境知识资源中心(Arid): Sensitive, wide-range and high-throughput quantification of cyclosporine in whole blood using ultra-performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody-conjugated magnetic immunoassay

Arid

DOI	10.1002/bmc.5128
	Sensitive, wide-range and high-throughput quantification of cyclosporine in whole blood using ultra-performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody-conjugated magnetic immunoassay
	Watanabe, Takuma; Tanaka, Ryota; Ono, Hiroyuki; Suzuki, Yosuke; Tatsuta, Ryosuke; Itoh, Hiroki
通讯作者	Tanaka, R (corresponding author), Oita Univ Hosp, Dept Clin Pharm, Hasama Machi, Yufu, Oita 8795593, Japan.
来源期刊	BIOMEDICAL CHROMATOGRAPHY
ISSN	0269-3879
EISSN	1099-0801
出版年	2021
卷号	35 期号:8
英文摘要	Because either trough or peak concentration at 2 h after administration is measured in routine therapeutic drug monitoring for cyclosporine A (CyA), a quantification method with a wide-range calibration curve capable of simultaneously measuring both concentrations is required. We developed a sensitive, wide-range and high-throughput quantification method for CyA in whole blood using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and compared patients' blood CyA levels measured by UPLC-MS/MS and antibody-conjugated magnetic immunoassay (ACMIA). Whole blood samples were prepared by solid-phase extraction using Oasis HLB mu Elution plate. The UPLC-MS/MS assay showed excellent linearity over a wide calibration range of 5-2500 ng/mL. Within-batch accuracy and precision as well as batch-to-batch accuracy and precision fulfilled the criteria of US Food and Drug Administration guidelines. The blood CyA concentrations measured by the UPLC-MS/MS assay correlated strongly with those measured by ACMIA. A Bland-Altman plot showed a fixed error between CyA concentrations measured by the two methods, and the concentrations measured by the UPLC-MS/MS method were consistently lower than those measured by ACMIA. We have succeeded to develop a sensitive, wide-range and high-throughput quantification method for CyA in whole blood using UPLC-MS/MS.
英文关键词	antibody‐ conjugated magnetic immunoassay cyclosporine therapeutic drug monitoring ultra‐ performance liquid chromatography– tandem mass spectrometry
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000641125700001
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349716
作者单位	[Watanabe, Takuma; Tanaka, Ryota; Ono, Hiroyuki; Tatsuta, Ryosuke; Itoh, Hiroki] Oita Univ Hosp, Dept Clin Pharm, Hasama Machi, Yufu, Oita 8795593, Japan; [Suzuki, Yosuke] Meiji Pharmaceut Univ, Dept Medicat Use Anal & Clin Res, Tokyo, Japan
推荐引用方式 GB/T 7714	Watanabe, Takuma,Tanaka, Ryota,Ono, Hiroyuki,et al. Sensitive, wide-range and high-throughput quantification of cyclosporine in whole blood using ultra-performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody-conjugated magnetic immunoassay[J],2021,35(8).
APA	Watanabe, Takuma,Tanaka, Ryota,Ono, Hiroyuki,Suzuki, Yosuke,Tatsuta, Ryosuke,&Itoh, Hiroki.(2021).Sensitive, wide-range and high-throughput quantification of cyclosporine in whole blood using ultra-performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody-conjugated magnetic immunoassay.BIOMEDICAL CHROMATOGRAPHY,35(8).
MLA	Watanabe, Takuma,et al."Sensitive, wide-range and high-throughput quantification of cyclosporine in whole blood using ultra-performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody-conjugated magnetic immunoassay".BIOMEDICAL CHROMATOGRAPHY 35.8(2021).