干旱区生态环境知识资源中心(Arid): A sensitive and efficient LC-MS/MS method for the bioanalysis of fosinopril diacid from human plasma and its application for a bioequivalence study in humans

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DOI	10.1002/bmc.5047
	A sensitive and efficient LC-MS/MS method for the bioanalysis of fosinopril diacid from human plasma and its application for a bioequivalence study in humans
	Bhende, Swati D.; Varanasi, Murali Balaram; Abbulu, Konde
通讯作者	Bhende, SD (corresponding author), Jawaharlal Nehru Technol Univ Hyderabad, Hyderabad 500085, Telangana, India.
来源期刊	BIOMEDICAL CHROMATOGRAPHY
ISSN	0269-3879
EISSN	1099-0801
出版年	2021
卷号	35 期号:5
英文摘要	Fosinopril diacid is an angiotensin converting enzyme inhibitor with efficient antihypertensive action. It is an active metabolic product formed in the body from hydrolysis of its prodrug Fosinopril. A sensitive, rapid method with high recovery for Fosinopril diacid from human plasma was developed. Solid-phase extraction technique employing Waters Oasis SPE cartridges gave clean samples with very high recovery of 97%. The analyte along with its internal standard (Benazepril hydrochloride) were chromatographed on an XTerra RP8 column (4.6 x 50 mm, 5 mu m) using methanol-ammonium acetate buffer (10 mm; 90:10, v/v) as the mobile phase. A triple quadrupole mass spectrometer equipped with electrospray ionization source operated in the negative ion mode was used for detection. Multiple reaction monitoring scan mode was used for monitoring the transitions from m/z 434.00 -> 237.15 for Fosinopril diacid and m/z 423.10 -> 174.00 for Benazepril hydrochloride. Beer-Lambert's law was obeyed in the range of 0.50-1,500.00 ng/ml (r = 0.9993). The stability of the drugs in human plasma and in stock solution was proved by performing stability tests as per US Food and Drug Administration guidelines. The method was successfully applied for a bioequivalence study of Fosinopril diacid in 36 healthy, adult, male volunteers under fasting conditions.
英文关键词	Benazepril hydrochloride bioequivalence study Fosinopril diacid human plasma LC-MS/MS solid-phase extraction
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000613875700001
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349714
作者单位	[Bhende, Swati D.] Jawaharlal Nehru Technol Univ Hyderabad, Hyderabad 500085, Telangana, India; [Varanasi, Murali Balaram] Sultan UI Uloom Coll Pharm, Hyderabad, Telangana, India; [Abbulu, Konde] CMR Coll Pharm, Hyderabad, Telangana, India
推荐引用方式 GB/T 7714	Bhende, Swati D.,Varanasi, Murali Balaram,Abbulu, Konde. A sensitive and efficient LC-MS/MS method for the bioanalysis of fosinopril diacid from human plasma and its application for a bioequivalence study in humans[J],2021,35(5).
APA	Bhende, Swati D.,Varanasi, Murali Balaram,&Abbulu, Konde.(2021).A sensitive and efficient LC-MS/MS method for the bioanalysis of fosinopril diacid from human plasma and its application for a bioequivalence study in humans.BIOMEDICAL CHROMATOGRAPHY,35(5).
MLA	Bhende, Swati D.,et al."A sensitive and efficient LC-MS/MS method for the bioanalysis of fosinopril diacid from human plasma and its application for a bioequivalence study in humans".BIOMEDICAL CHROMATOGRAPHY 35.5(2021).