干旱区生态环境知识资源中心(Arid): Revival of Anhydrobiotic Cyanobacterium Biofilms Exposed to Space Vacuum and Prolonged Dryness: Implications for Future Missions beyond Low Earth Orbit

Arid

DOI	10.1089/ast.2020.2359
	Revival of Anhydrobiotic Cyanobacterium Biofilms Exposed to Space Vacuum and Prolonged Dryness: Implications for Future Missions beyond Low Earth Orbit
	Mosca, Claudia; Fagliarone, Claudia; Napoli, Alessandro; Rabbow, Elke; Rettberg, Petra; Billi, Daniela
通讯作者	Billi, D (corresponding author), Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, I-00133 Rome, Italy.
来源期刊	ASTROBIOLOGY
ISSN	1531-1074
EISSN	1557-8070
出版年	2021
卷号	21 期号:5 页码:541-550
英文摘要	Dried biofilms of Chroococcidiopsis sp. CCMEE 029 were revived after a 672-day exposure to space vacuum outside the International Space Station during the EXPOSE-R2 space mission. After retrieval, they were air-dried stored for 3.5 years. Space vacuum reduced cell viability and increased DNA damage compared to air-dried storage for 6 years under laboratory conditions. Long exposure times to space vacuum and extreme dryness decrease the changes of survival that ultimately depend on DNA damage repair upon rehydration, and hence, an in silico analysis of Chroococcidiopsis sp. CCMEE 029's genome was performed with a focus on DNA repair pathways. The analysis identified a high number of genes that encode proteins of the homologous recombination RecF pathway and base excision repair that were over-expressed during 1 and 6 h rehydration of space-vacuum exposed biofilms. This suggests that Chroococcidiopsis developed a survival strategy against desiccation, with DNA repair playing a key role, which allowed the revival of biofilms exposed to space vacuum. Unravelling how long anhydrobiotic cyanobacteria can persist under space vacuum followed by prolonged air-dried storage is relevant to future astrobiological experiments that use space platforms and might require prolonged air-dried storage of the exposed samples before retrieval to Earth.
英文关键词	Biofilm Organisms Surfing Space DNA repair Extreme dryness EXPOSE-R2 space mission
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000647638900004
WOS关键词	DNA-REPAIR ; DESERT CYANOBACTERIUM ; RESISTANCE ; UV ; LIFE ; CHROOCOCCIDIOPSIS ; RECOMBINATION ; HABITABILITY ; DESICCATION ; SURVIVAL
WOS类目	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS研究方向	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349596
作者单位	[Mosca, Claudia; Fagliarone, Claudia; Napoli, Alessandro; Billi, Daniela] Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, I-00133 Rome, Italy; [Rabbow, Elke; Rettberg, Petra] German Aerosp Ctr, Inst Aerosp Med, Cologne, Germany
推荐引用方式 GB/T 7714	Mosca, Claudia,Fagliarone, Claudia,Napoli, Alessandro,et al. Revival of Anhydrobiotic Cyanobacterium Biofilms Exposed to Space Vacuum and Prolonged Dryness: Implications for Future Missions beyond Low Earth Orbit[J],2021,21(5):541-550.
APA	Mosca, Claudia,Fagliarone, Claudia,Napoli, Alessandro,Rabbow, Elke,Rettberg, Petra,&Billi, Daniela.(2021).Revival of Anhydrobiotic Cyanobacterium Biofilms Exposed to Space Vacuum and Prolonged Dryness: Implications for Future Missions beyond Low Earth Orbit.ASTROBIOLOGY,21(5),541-550.
MLA	Mosca, Claudia,et al."Revival of Anhydrobiotic Cyanobacterium Biofilms Exposed to Space Vacuum and Prolonged Dryness: Implications for Future Missions beyond Low Earth Orbit".ASTROBIOLOGY 21.5(2021):541-550.