干旱区生态环境知识资源中心(Arid): Perchlorate Salts Exert a Dominant, Deleterious Effect on the Structure, Stability, and Activity of alpha-Chymotrypsin

Arid

DOI	10.1089/ast.2020.2223
	Perchlorate Salts Exert a Dominant, Deleterious Effect on the Structure, Stability, and Activity of alpha-Chymotrypsin
	Gault, Stewart; Cockell, Charles S.
通讯作者	Gault, S (corresponding author), Univ Edinburgh, Ctr Astrobiol, SUPA Sch Phys & Astron, James Clerk Maxwell Bldg,Peter Guthrie Tait Rd, Edinburgh EH9 3FD, Midlothian, Scotland.
来源期刊	ASTROBIOLOGY
ISSN	1531-1074
EISSN	1557-8070
出版年	2021
卷号	21 期号:4 页码:405-412
英文摘要	The presence of perchlorate ions on Mars raises the question of how these ions influence the biochemistry of any contaminant life introduced into the martian environment, or what selection pressures perchlorate ions exert on any environment that contains these ions, such as the Atacama Desert. In this study, we investigated the structure, stability, and enzyme activity of the model enzyme alpha-chymotrypsin in the presence of five Mars relevant salts, MgSO4, MgCl2, Mg(ClO4)(2), Ca(ClO4)(2), and NaClO4. We found that all the perchlorate salts reduced the enzyme activity of alpha-chymotrypsin in a concentration-dependent manner, with Mg(ClO4)(2) and Ca(ClO4)(2) having the greatest effect. This observation extends to our structural studies, which show that 1 M Mg(ClO4)(2) and Ca(ClO4)(2) greatly alter the tertiary structural environment of alpha-chymotrypsin. We also found that all the perchlorate salts assayed reduced the melting temperature of alpha-chymotrypsin, whereas the sulfate and chloride salts were able to increase the protein melting temperature. We also demonstrated that a brine containing both perchlorate and sulfate ions exerts the same deleterious effects on alpha-chymotrypsin's melting temperature and enzyme activity as that of a perchlorate-only brine. This suggests that the perchlorate salts exert a dominant, deleterious effect on protein biochemistry. These results indicate that although perchlorate salts are beneficial to the presence of liquid water due to low eutectic points, they also hamper the habitability of their own environment. Life in such brines would, therefore, have to adapt its cellular machinery to the perchlorate ion's presence or find a way of excluding it from said machinery.
英文关键词	Perchlorate Mars Enzymology Biochemistry Protein Astrobiology
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000634918800003
WOS类目	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS研究方向	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349594
作者单位	[Gault, Stewart; Cockell, Charles S.] Univ Edinburgh, Ctr Astrobiol, SUPA Sch Phys & Astron, James Clerk Maxwell Bldg,Peter Guthrie Tait Rd, Edinburgh EH9 3FD, Midlothian, Scotland
推荐引用方式 GB/T 7714	Gault, Stewart,Cockell, Charles S.. Perchlorate Salts Exert a Dominant, Deleterious Effect on the Structure, Stability, and Activity of alpha-Chymotrypsin[J],2021,21(4):405-412.
APA	Gault, Stewart,&Cockell, Charles S..(2021).Perchlorate Salts Exert a Dominant, Deleterious Effect on the Structure, Stability, and Activity of alpha-Chymotrypsin.ASTROBIOLOGY,21(4),405-412.
MLA	Gault, Stewart,et al."Perchlorate Salts Exert a Dominant, Deleterious Effect on the Structure, Stability, and Activity of alpha-Chymotrypsin".ASTROBIOLOGY 21.4(2021):405-412.