干旱区生态环境知识资源中心(Arid): Zafaria cholistanensis gen. nov. sp. nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan

Arid

DOI	10.1007/s00203-020-02176-4
	Zafaria cholistanensis gen. nov. sp. nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan
	Amin, Arshia; Ahmed, Iftikhar; Khalid, Nauman; Schumann, Peter; Busse, Hans-Jurgen; Khan, Inam Ullah; Ali, Ahmad; Li, Shuai; Li, Wen-Jun
通讯作者	Li, WJ (corresponding author), Sun Yat Sen Univ, State Key Lab Biocontrol, Guangdong Prov Key Lab Plant Resources, Sch Life Sci, Guangzhou 510275, Peoples R China. ; Li, WJ (corresponding author), Sun Yat Sen Univ, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Sch Life Sci, Guangzhou 510275, Peoples R China. ; Ahmed, I (corresponding author), Natl Agr Res Ctr NARC, Natl Culture Collect Pakistan NCCP, Bioresources Conservat Inst BCI, Pk Rd, Islamabad 45500, Pakistan.
来源期刊	ARCHIVES OF MICROBIOLOGY
ISSN	0302-8933
EISSN	1432-072X
出版年	2021
卷号	203 期号:4 页码:1717-1729
英文摘要	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664(T), was isolated from Cholistan desert, Pakistan. Cells of strain NCCP-1664(T) were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 degrees C (optimum 37 degrees C) and could tolerate 0-16% NaCl (optimum 2%). Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664(T) belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa. DNA-DNA relatedness values of strain NCCP-1664(T) with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively. DNA G + C content of strain NCCP-1664(T) was 70.0 mol%. Chemotaxonomic data of strain NCCP-1664(T) showed the peptidoglycan type as A3 alpha l-Lys-l -Ala; menaquinones as MK-9(H-2) (67%), MK-8(H-2) (32%) and MK-7(H-2) (1%), major fatty acids as anteiso -C-15:0 (51.2%), anteiso-C-17:0 (9.6%) and C-18:1 omega 9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids. The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp. nov. is proposed with the type strain NCCP-1664(T) (= DSM 29936(T) = KCTC 39549(T)).
英文关键词	Cholistan desert Peptidoglycan type A3 alpha Thermotolerant Halotolerant
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000608672100003
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349581
作者单位	[Amin, Arshia; Li, Shuai; Li, Wen-Jun] Sun Yat Sen Univ, State Key Lab Biocontrol, Guangdong Prov Key Lab Plant Resources, Sch Life Sci, Guangzhou 510275, Peoples R China; [Amin, Arshia; Li, Shuai; Li, Wen-Jun] Sun Yat Sen Univ, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Sch Life Sci, Guangzhou 510275, Peoples R China; [Amin, Arshia; Ahmed, Iftikhar; Ali, Ahmad] Natl Agr Res Ctr NARC, Natl Culture Collect Pakistan NCCP, Bioresources Conservat Inst BCI, Pk Rd, Islamabad 45500, Pakistan; [Amin, Arshia] Capital Univ Sci & Technol, Dept Bioinformat & Biosci, Islamabad, Pakistan; [Khalid, Nauman] Univ Management & Technol, Sch Food & Agr Sci, Lahore 54000, Pakistan; [Schumann, Peter] Leibniz Inst DSMZ Deutsch Sammlung Mikroorganisme, Inhoffenstr 7B, D-38124 Braunschweig, Germany; [Busse, Hans-Jurgen] Vet Med Univ Wien, Inst Mikrobiol, Vet Pl 1, A-1210 Vienna, Austria; [Khan, Inam Ullah] Gomal Univ, Inst Microbiol, Fac Vet & Anim Sci, Dera Ismail Khan, Pakistan
推荐引用方式 GB/T 7714	Amin, Arshia,Ahmed, Iftikhar,Khalid, Nauman,et al. Zafaria cholistanensis gen. nov. sp. nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan[J],2021,203(4):1717-1729.
APA	Amin, Arshia.,Ahmed, Iftikhar.,Khalid, Nauman.,Schumann, Peter.,Busse, Hans-Jurgen.,...&Li, Wen-Jun.(2021).Zafaria cholistanensis gen. nov. sp. nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.ARCHIVES OF MICROBIOLOGY,203(4),1717-1729.
MLA	Amin, Arshia,et al."Zafaria cholistanensis gen. nov. sp. nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan".ARCHIVES OF MICROBIOLOGY 203.4(2021):1717-1729.