干旱区生态环境知识资源中心(Arid): Soil microbial DNA concentration is a powerful indicator for estimating soil microbial biomass C and N across arid and semi-arid regions in northern China

Arid

DOI	10.1016/j.apsoil.2020.103869
	Soil microbial DNA concentration is a powerful indicator for estimating soil microbial biomass C and N across arid and semi-arid regions in northern China
	Gong, Haiyang; Du, Qiajun; Xie, Shubin; Hu, Weigang; Akram, Muhammad Adnan; Hou, Qingqing; Dong, Longwei; Sun, Ying; Manan, Abdul; Deng, Yan; Ran, Jinzhi; Deng, Jianming
通讯作者	Ran, JZ ; Deng, JM (corresponding author), Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, 222 South Tianshui Rd, Lanzhou 730000, Peoples R China.
来源期刊	APPLIED SOIL ECOLOGY
ISSN	0929-1393
EISSN	1873-0272
出版年	2021
卷号	160
英文摘要	Soil microbes exert critical control over ecosystem functions by regulating organic matter decomposition and nutrient cycling. Diverse methods have been adopted to estimate the soil microbial biomass carbon (C-mic) and nitrogen (N-mic), largely based on fumigation and respiration or biomarkers, such as phospholipids and ATP. Although soil microbial DNA is also an important biomarker, whether this biomarker can be used to estimate C-mic and N-mic at a large scale in arid and semi-arid regions that remains unclear. To explore this, the 124 soil samples collected from 28 different vegetation/microhabitat types in diverse ecosystems (i.e., desert, cropland, typical steppe, meadow steppe and forest) across arid and semi-arid regions in northern China; then, the strong linear relationships were developed among soil microbial DNA concentrations with the C-mic and N-mic contents estimated by the classic chloroform fumigation-extraction (CFE) method. The results showed that the soil microbial DNA concentrations were linearly related to the observed C-mic (r = 0.99) and N-mic (r = 0.97) across the diverse vegetation types, although soil microbial biomass presented an enormous fluctuation. Moreover, based on the above-mentioned coefficients of the linear relationship, it was further inferred that the mean proportions of carbon and nitrogen elements in DNA were 0.99% and 3.70% for C-mic and N-mic, respectively, when assuming the G-C base-pair proportion is 50% in double-stranded DNA. In conclusion, the study proved that soil microbial DNA as a powerful indicator may provide convenient and efficient estimates for C-mic and N-mic in arid and semiarid systems. Although this method still needs to be further improved and examined at a larger scale, it provides a good addition to the already existing methods to determine microbial biomass.
英文关键词	Microbial biomass Soil DNA Vegetation/microhabitat types Fumigation methods GC content
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000618081000004
WOS类目	Soil Science
WOS研究方向	Agriculture
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349510
作者单位	[Gong, Haiyang; Du, Qiajun; Xie, Shubin; Hu, Weigang; Akram, Muhammad Adnan; Dong, Longwei; Sun, Ying; Manan, Abdul; Deng, Yan; Ran, Jinzhi; Deng, Jianming] Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, 222 South Tianshui Rd, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Gong, Haiyang,Du, Qiajun,Xie, Shubin,et al. Soil microbial DNA concentration is a powerful indicator for estimating soil microbial biomass C and N across arid and semi-arid regions in northern China[J]. 兰州大学,2021,160.
APA	Gong, Haiyang.,Du, Qiajun.,Xie, Shubin.,Hu, Weigang.,Akram, Muhammad Adnan.,...&Deng, Jianming.(2021).Soil microbial DNA concentration is a powerful indicator for estimating soil microbial biomass C and N across arid and semi-arid regions in northern China.APPLIED SOIL ECOLOGY,160.
MLA	Gong, Haiyang,et al."Soil microbial DNA concentration is a powerful indicator for estimating soil microbial biomass C and N across arid and semi-arid regions in northern China".APPLIED SOIL ECOLOGY 160(2021).