干旱区生态环境知识资源中心(Arid): Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii x nigra

Arid

DOI	10.1007/s13595-021-01038-3
	Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii x nigra
	Wang, Yang; Yang, Yunli; Wang, Fusen; Wang, Guangyu; Wang, Chu; Wang, Wei; Chen, Kun; Gu, Chenrui; Yu, Qibin; Jiang, Jing
通讯作者	Jiang, J (corresponding author), Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, 26 Hexing Rd, Harbin 150040, Peoples R China.
来源期刊	ANNALS OF FOREST SCIENCE
ISSN	1286-4560
EISSN	1297-966X
出版年	2021
卷号	78 期号:2
英文摘要	Key message Late-embryogenesis-abundant proteins (LEA) typically accumulate to high levels during seed dehydration and have an important role in the plant response to abiotic stresses. Transgenic hybrid poplars of 9-year-old TaLEAlines showed adaptation to drought and saline-alkali conditions. The exogenous gene was expressed normally, and the T-DNA insertion sites were identified on different chromosomes. Context Improving the survival rate of afforested species is essential for forest production in saline-alkali areas. Transgenic hybrid poplars could provide potential genetic materials for adaptation to semi-arid areas. Aims We investigated the growth stability and adaptability of 9-year-old TaLEA transgenic lines of Populus simonii x nigra, as well as the position of T-DNA insertion and the stability of exogenous genes. Methods Transgenic poplar field trials were carried out using a multi-site joint analysis. Molecular characteristics and the T-DNA insertion sites were identified by genome resequencing. Results The XL-9, XL-7, and XL-13 preferred lines consistently showed the highest and most stable growth across three testing sites. The exogenous gene was integrated into the genome chromosome and was expressed normally in the XL-1, XL-7, and XL-9 lines after 9 years. Conclusion XL-9, XL-7, and XL-13 are the preferred lines for subsequent production tests. Transgenic hybrid poplar lines can be selected for adaptability 2 years after field experiments. The results of this study will provide further guidance for testing of transgenic trees for future forest production.
英文关键词	Populus simonii × nigra Late-embryogenesis-abundant proteins Abiotic stress Field experiment
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000640563200002
WOS类目	Forestry
WOS研究方向	Forestry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349465
作者单位	[Wang, Yang; Yang, Yunli; Wang, Chu; Wang, Wei; Chen, Kun; Gu, Chenrui; Jiang, Jing] Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, 26 Hexing Rd, Harbin 150040, Peoples R China; [Wang, Fusen] Heilongjiang Acad Forestry, Qiqihar Branch, Qiqihar, Peoples R China; [Wang, Guangyu] Heilongjiang Jiamao Garden Construct Co Ltd, Daqing, Peoples R China; [Yu, Qibin] Univ Florida, Gainesville, FL USA
推荐引用方式 GB/T 7714	Wang, Yang,Yang, Yunli,Wang, Fusen,et al. Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii x nigra[J],2021,78(2).
APA	Wang, Yang.,Yang, Yunli.,Wang, Fusen.,Wang, Guangyu.,Wang, Chu.,...&Jiang, Jing.(2021).Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii x nigra.ANNALS OF FOREST SCIENCE,78(2).
MLA	Wang, Yang,et al."Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii x nigra".ANNALS OF FOREST SCIENCE 78.2(2021).