干旱区生态环境知识资源中心(Arid): Microscopic and Metagenomic Evidence for Eukaryotic Microorganisms Associated with Atacama Desert Populations of Giant Equisetum

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	Microscopic and Metagenomic Evidence for Eukaryotic Microorganisms Associated with Atacama Desert Populations of Giant Equisetum
	Satjarak, Anchittha; Piotrowski, Michael J.; Graham, Linda E.; Trest, Marie T.; Wilcox, Lee W.; Knack, Jennifer J.; Cook, Martha E.; Arancibia-Avila, Patricia
通讯作者	Graham, LE (corresponding author), Univ Wisconsin, Dept Bot, Madison, WI 53706 USA.
来源期刊	AMERICAN FERN JOURNAL
ISSN	0002-8444
EISSN	1938-422X
出版年	2021
卷号	111 期号:2 页码:86-109
英文摘要	Understanding features that fostered the persistence of Equisetum-Earth's oldest extant vascular plant genus-since Mesozoic times and through episodes of significant global environmental change, is of current interest in view of modern challenges to plant survival. In addition to known structural and physiological adaptations, we hypothesized that microscopy and shotgun metagenomic sequencing might reveal eukaryotic microorganisms such as fungi that may aid Equisetum survival. Here, we report evidence for several lineages of eukaryotic microbes associated with giant Equisetum xylochaetum, which dominates vegetation in saline streambeds of remote valleys in the hyper-arid Atacama Desert, Chile. Plant material was collected and fieldpreserved at two comparatively low-disturbance sites; DNA extracted in Chile using low-shear methods was later sequenced, 18S and 28S rDNA taxonomic marker sequences were selected for SILVAngs classification, allowing comparisons to eukaryotic microorganisms previously inferred for earlier-diverging plant lineages. SEM, fluorescence microscopy, and/or LM of toluidine bluestained sections of roots indicated protists, epiphytic and endophytic fungi, and cortical nematodes. Eukaryotic genera inferred from 18S rDNA at.100X mean sequencing depth included the ciliate Engelmanniella, hyphal chytrid Monoblepharella, predatory ascomycete Cephaliophora, a salpingoecid choanoflagellate, and an annelid worm. 23S rDNA sequences indicated ascomycete Capnodiales fungi at one site and four types of Pezizomycotina fungi at the other. No evidence for vesicular-arbuscular mycorrhizal fungi was found, but we hypothesized that Equisetum may benefit from other types of fungal associations, some possibly inherited from ancestral plant lineages.
英文关键词	horsetails metagenomic sequencing scouring rushes seedless plant microbiomes
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000661251000003
WOS关键词	VESICULAR-ARBUSCULAR MYCORRHIZAS ; ROOT MICROBIOME ; CHILE INSIGHTS ; PLANT ; DIVERSITY ; ANATOMY ; CLASSIFICATION ; COLONIZATION ; COMMUNITIES ; HORSETAILS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349428
作者单位	[Satjarak, Anchittha] Chulalongkorn Univ, Fac Sci, Dept Bot, Plants Thailand Res Unit, Bangkok 10330, Thailand; [Piotrowski, Michael J.; Graham, Linda E.; Trest, Marie T.; Wilcox, Lee W.] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA; [Knack, Jennifer J.] Univ Minnesota, Dept Biol, Duluth, MN 55812 USA; [Cook, Martha E.] Illinois State Univ, Sch Biol Sci, Normal, IL 61790 USA; [Arancibia-Avila, Patricia] Univ Bio Bio, Dept Basic Sci, Chillan, Chile
推荐引用方式 GB/T 7714	Satjarak, Anchittha,Piotrowski, Michael J.,Graham, Linda E.,et al. Microscopic and Metagenomic Evidence for Eukaryotic Microorganisms Associated with Atacama Desert Populations of Giant Equisetum[J],2021,111(2):86-109.
APA	Satjarak, Anchittha.,Piotrowski, Michael J..,Graham, Linda E..,Trest, Marie T..,Wilcox, Lee W..,...&Arancibia-Avila, Patricia.(2021).Microscopic and Metagenomic Evidence for Eukaryotic Microorganisms Associated with Atacama Desert Populations of Giant Equisetum.AMERICAN FERN JOURNAL,111(2),86-109.
MLA	Satjarak, Anchittha,et al."Microscopic and Metagenomic Evidence for Eukaryotic Microorganisms Associated with Atacama Desert Populations of Giant Equisetum".AMERICAN FERN JOURNAL 111.2(2021):86-109.