干旱区生态环境知识资源中心(Arid): Structure and functional properties of the cold-adapted catalase from Acinetobacter sp. Ver3 native to the Atacama plateau in northern Argentina

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DOI	10.1107/S2059798321000929
	Structure and functional properties of the cold-adapted catalase from Acinetobacter sp. Ver3 native to the Atacama plateau in northern Argentina
	Sartorio, Mariana G.; Cortez, Nestor; Gonzalez, Javier M.
通讯作者	Gonzalez, JM (corresponding author), Univ Nacl Santiago del Estero UNSE, Inst Bionanotecnol NOA INBIONATEC CONICET, RN9,Km1125,G4206XCP, Santiago Del Estero, Argentina.
来源期刊	ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
ISSN	2059-7983
出版年	2021
卷号	77 页码:369-379
英文摘要	Heme catalases remove hydrogen peroxide by catalyzing its dismutation into water and molecular oxygen, thereby protecting the cell from oxidative damage. The Atacama plateau in northern Argentina, located 4000 m above sea level, is a desert area characterized by extreme UV radiation, high salinity and a large temperature variation between day and night. Here, the heme catalase KatE1 from an Atacama Acinetobacter sp. isolate was cloned, expressed and purified, with the aim of investigating its extremophilic properties. Kinetic and stability assays indicate that KatE1 is maximally active at 50 degrees C in alkaline media, with a nearly unchanged specific activity between 0 degrees C and 40 degrees C in the pH range 5.5-11.0. In addition, its three-dimensional crystallographic structure was solved, revealing minimal structural differences compared with its mesophilic and thermophilic analogues, except for a conserved methionine residue on the distal heme side, which is proposed to comprise a molecular adaptation to oxidative damage.
英文关键词	catalases Atacama plateau Acinetobacter cold-adapted enzymes
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000625172500011
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349252
作者单位	[Sartorio, Mariana G.; Cortez, Nestor] Univ Nacl Rosario UNR, Inst Biol Mol & Celular Rosario IBR CONICET, Suipacha 531,S2002LRK, Rosario, Santa Fe, Argentina; [Gonzalez, Javier M.] Univ Nacl Santiago del Estero UNSE, Inst Bionanotecnol NOA INBIONATEC CONICET, RN9,Km1125,G4206XCP, Santiago Del Estero, Argentina; [Sartorio, Mariana G.] Washington Univ, Dept Mol Microbiol, St Louis, MO 63110 USA
推荐引用方式 GB/T 7714	Sartorio, Mariana G.,Cortez, Nestor,Gonzalez, Javier M.. Structure and functional properties of the cold-adapted catalase from Acinetobacter sp. Ver3 native to the Atacama plateau in northern Argentina[J],2021,77:369-379.
APA	Sartorio, Mariana G.,Cortez, Nestor,&Gonzalez, Javier M..(2021).Structure and functional properties of the cold-adapted catalase from Acinetobacter sp. Ver3 native to the Atacama plateau in northern Argentina.ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,77,369-379.
MLA	Sartorio, Mariana G.,et al."Structure and functional properties of the cold-adapted catalase from Acinetobacter sp. Ver3 native to the Atacama plateau in northern Argentina".ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 77(2021):369-379.