干旱区生态环境知识资源中心(Arid): Genome-wide association studies of callus differentiation for the desert tree, Populus euphratica

Arid

DOI	10.1093/treephys/tpaa098
	Genome-wide association studies of callus differentiation for the desert tree, Populus euphratica
	Zhang, Qianru; Su, Zhifang; Guo, Yunqian; Zhang, Shilong; Jiang, Libo; Wu, Rongling
通讯作者	Jiang, LB (corresponding author), Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China. ; Jiang, LB (corresponding author), Beijing Forestry Univ, Coll Biol Sci & Technol, Ctr Computat Biol, Beijing 100083, Peoples R China.
来源期刊	TREE PHYSIOLOGY
ISSN	0829-318X
EISSN	1758-4469
出版年	2020
卷号	40 期号:12 页码:1762-1777
英文摘要	Callus differentiation is a key developmental process in plant regeneration from cells. A better understanding of the genetic architecture of callus differentiation timing can help improve tissue transformation and the efficiency of artificial propagation. In this study, we investigated genotypic variation in callus differentiation capacity among 297 diverse P. euphratica trees sampled from a natural population. We employed a genome-wide association study (GWAS) of binary and growth-based parameters to identify loci and characterize the genetic architecture and genetic network underlying regulation of callus differentiation in P. euphratica. The results of this GWAS experiment suggested potential associations controlling whether the callus could differentiate and the process of callus differentiation. We identified multiple significant quantitative trait loci (QTLs), including the genes LOG1 and LOG7 and a locus containing WOX1. We reconstructed a genetic network that visualizes how each QTL interacts uniquely with other variants, and several core QTLs were detected that are involved in the degree of callus differentiation, providing potential targets for selection. This study represents one of the first to identify genetic variants affecting callus differentiation in a forest tree. Our results suggest that callus differentiation may be a typical qualitative-quantitative trait controlled by a major gene as well as polygenes across the genome of P. euphratica. This GWAS will help to design more complex and specific molecular tools for systematically manipulating organ regeneration.
英文关键词	callus differentiation Euphrates poplar genetic network growth equation quantitative trait loci
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000607844500009
WOS关键词	PLANT-REGENERATION ; GENETIC ARCHITECTURE ; SHOOT REGENERATION ; DIRECT ACTIVATION ; IN-VITRO ; GROWTH ; EXPRESSION ; INDUCTION ; LINKAGE ; PATHWAY
WOS类目	Forestry
WOS研究方向	Forestry
来源机构	北京林业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349210
作者单位	[Zhang, Qianru; Zhang, Shilong; Jiang, Libo; Wu, Rongling] Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China; [Zhang, Qianru; Su, Zhifang; Guo, Yunqian; Zhang, Shilong; Jiang, Libo; Wu, Rongling] Beijing Forestry Univ, Coll Biol Sci & Technol, Ctr Computat Biol, Beijing 100083, Peoples R China; [Wu, Rongling] Penn State Univ, Ctr Stat Genet, Hershey, PA 17033 USA
推荐引用方式 GB/T 7714	Zhang, Qianru,Su, Zhifang,Guo, Yunqian,et al. Genome-wide association studies of callus differentiation for the desert tree, Populus euphratica[J]. 北京林业大学,2020,40(12):1762-1777.
APA	Zhang, Qianru,Su, Zhifang,Guo, Yunqian,Zhang, Shilong,Jiang, Libo,&Wu, Rongling.(2020).Genome-wide association studies of callus differentiation for the desert tree, Populus euphratica.TREE PHYSIOLOGY,40(12),1762-1777.
MLA	Zhang, Qianru,et al."Genome-wide association studies of callus differentiation for the desert tree, Populus euphratica".TREE PHYSIOLOGY 40.12(2020):1762-1777.