干旱区生态环境知识资源中心(Arid): Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers

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DOI	10.1016/j.sjbs.2020.08.020
	Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers
	Ismail, Muhammad; Ahmad, Aftab; Nadeem, Muhammad; Javed, Muhammad Asif; Khan, Sultan Habibullah; Khawaish, Iqra; Sthanadar, Aftab Alam; Qari, Sameer H.; Alghanem, Suliman M.; Khan, Khalid Ali; Khan, Muhammad Fiaz; Qamer, Samina
通讯作者	Ahmad, A (corresponding author), Univ Agr Faisalabad, Ctr Adv Studies Agr & Food Secur CAS AFS, Faisalabad, Pakistan.
来源期刊	SAUDI JOURNAL OF BIOLOGICAL SCIENCES
ISSN	1319-562X
EISSN	2213-7106
出版年	2020
卷号	27 期号:12 页码:3735-3742
英文摘要	Acacia species are very important tree species in tropical and subtropical countries of the World for their economic and medicinal benefits. Precise identification of Acacia is very important to distinguish the invasive species from rare species however, it is difficult to differentiate Acacia species based on morphological charcters. In addition, precise identification is also important for wood charcterization in the forest industry as these species are declining due to illegal logging and deforestation. To overcome thsese limitations of morphological identification, DNA barcoding is being used as an efficient and quick approach for precise identification of tree species. In this study, we selected two chloroplast and plastid base DNA markers (rbcL and matK) for the identification of five selected tree species of Acacia (A. albida, A. ampliceps, A. catechu, A. coriacea and A. tortilis). The genomic DNA of the selected Acacia species was extracted, amplified through PCR using specific primers and subsequently sequenced through Sanger sequencing. In matK DNA marker the average AT nucleotide contents were higher (59.46%) and GC contents were lower (40.44%) as compared to the AT (55.40%) and GC content (44.54%) in rbcL marker. The means genetic distance K2P between the Acacia species was higher in matK (0.704%) as compared to rbcL (0.230%). All Acacia species could be identified based on unique SNPs profile. Based on SNP data profiles, DNA sequence based scannable QR codes were developed for accurate identification of Acacia species. The phylogenetic analysis based on both markers (rbcL and matK) showed that both A. coriacea and A. tortilis were closely related with each other and clustered in the same group while other two species A. albida and A. catechu were grouped together. The specie A. ampliceps remained ungrouped distantly, compared with other four species. These finding highlights the potential of DNA barcoding for efficient and reproducible identification of Acacia species. (C) 2020 The Author(s). Published by Elsevier B.V.
英文关键词	Desertification Acacia Genetic diversity Afforestation DNA barcoding
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000596396100010
WOS关键词	IDENTIFICATION ; POLYPLOIDY
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349177
作者单位	[Ismail, Muhammad; Javed, Muhammad Asif] Univ Agr Faisalabad, Dept Forestry & Range Management, Fac Agr, Faisalabad, Pakistan; [Ahmad, Aftab; Nadeem, Muhammad; Javed, Muhammad Asif; Khan, Sultan Habibullah; Khawaish, Iqra] Univ Agr Faisalabad, Ctr Adv Studies Agr & Food Secur CAS AFS, Faisalabad, Pakistan; [Ahmad, Aftab; Nadeem, Muhammad; Khawaish, Iqra] Univ Agr Faisalabad, Dept Biochem, Fac Sci, Faisalabad, Pakistan; [Khan, Sultan Habibullah] Univ Agr Faisalabad, Ctr Agr Biochem & Biotechnol CABB, Faisalabad, Pakistan; [Sthanadar, Aftab Alam] Higher Educ Dept, Peshawar, Pakistan; [Qari, Sameer H.] Umm Al Qura Univ, Univ Coll, Biol Dept, Mecca, Saudi Arabia; [Alghanem, Suliman M.] Tabuk Univ, Fac Sci, Biol Dept, Tabuk 71491, Saudi Arabia; [Khan, Khalid Ali] King Khalid Univ, Res Ctr Adv Mat Sci RCAMS, POB 9004, Abha 61413, Saudi Arabia; [Khan, Khalid Ali] King Khalid Univ, Fac Sci, Unit Bee Res & Honey Prod, POB 9004, Abha 61413, Saudi Arabia; [Khan, Khalid Ali] King Khalid Univ, Fac Sci, Biol Dept, POB ...
推荐引用方式 GB/T 7714	Ismail, Muhammad,Ahmad, Aftab,Nadeem, Muhammad,et al. Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers[J],2020,27(12):3735-3742.
APA	Ismail, Muhammad.,Ahmad, Aftab.,Nadeem, Muhammad.,Javed, Muhammad Asif.,Khan, Sultan Habibullah.,...&Qamer, Samina.(2020).Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers.SAUDI JOURNAL OF BIOLOGICAL SCIENCES,27(12),3735-3742.
MLA	Ismail, Muhammad,et al."Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers".SAUDI JOURNAL OF BIOLOGICAL SCIENCES 27.12(2020):3735-3742.