干旱区生态环境知识资源中心(Arid): De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing

Arid

DOI	10.1371/journal.pone.0244222
	De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing
	Yan, Dongqing; Ren, Jing; Liu, Jiamei; Ding, Yu; Niu, Jianming
通讯作者	Niu, JM (corresponding author), Inner Mongolia Univ, Sch Ecol & Environm, Hohhot, Peoples R China. ; Niu, JM (corresponding author), Minist Sci & Technol, Inner Mongolia Key Lab Grassland Ecol, Hohhot, Peoples R China. ; Niu, JM (corresponding author), Minist Sci & Technol, Candidate State Key Lab, Hohhot, Peoples R China. ; Niu, JM (corresponding author), Minist Educ, Key Lab Ecol & Resource Use Mongolian Plateau, Hohhot, Peoples R China.
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2020
卷号	15 期号:12
英文摘要	Grassland is one of the most widely-distributed ecosystems on Earth and provides a variety of ecosystem services. Grasslands, however, currently suffer from severe degradation induced by human activities, overgrazing pressure and climate change. In the present study, we explored the transcriptome response of Stipa breviflora, a dominant species in the desert steppe, to grazing through transcriptome sequencing, the development of simple sequence repeat (SSR) markers, and analysis of genetic diversity. De novo assembly produced 111,018 unigenes, of which 88,164 (79.41%) unigenes were annotated. A total of 686 unigenes showed significantly different expression under grazing, including 304 and 382 that were upregulated and downregulated, respectively. These differentially expressed genes (DEGs) were significantly enriched in the alpha-linolenic acid metabolism and plant-pathogen interaction pathways. Based on transcriptome sequencing data, we developed eight SSR molecular markers and investigated the genetic diversity of S. breviflora in grazed and ungrazed sites. We found that a relatively high level of S. breviflora genetic diversity occurred under grazing. The findings of genes that improve resistance to grazing are helpful for the restoration, conservation, and management of desert steppe.
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000603071400050
WOS关键词	SOIL PROPERTIES ; INNER-MONGOLIA ; RNA-SEQ ; GRASSLAND ; RESISTANCE ; EXPRESSION ; CHINA ; IDENTIFICATION ; CHECKLIST ; GRASSES
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349125
作者单位	[Yan, Dongqing; Ren, Jing; Liu, Jiamei; Ding, Yu; Niu, Jianming] Inner Mongolia Univ, Sch Ecol & Environm, Hohhot, Peoples R China; [Niu, Jianming] Minist Sci & Technol, Inner Mongolia Key Lab Grassland Ecol, Hohhot, Peoples R China; [Niu, Jianming] Minist Sci & Technol, Candidate State Key Lab, Hohhot, Peoples R China; [Niu, Jianming] Minist Educ, Key Lab Ecol & Resource Use Mongolian Plateau, Hohhot, Peoples R China
推荐引用方式 GB/T 7714	Yan, Dongqing,Ren, Jing,Liu, Jiamei,et al. De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing[J],2020,15(12).
APA	Yan, Dongqing,Ren, Jing,Liu, Jiamei,Ding, Yu,&Niu, Jianming.(2020).De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing.PLOS ONE,15(12).
MLA	Yan, Dongqing,et al."De novo assembly, annotation, marker discovery, and genetic diversity of the Stipa breviflora Griseb. (Poaceae) response to grazing".PLOS ONE 15.12(2020).