干旱区生态环境知识资源中心(Arid): The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment

Arid

DOI	10.7717/peerj.10342
	The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment
	Li, Yanlin; Chen, Jixiang; Wang, Yonggang; Ma, Dan; Rui, Wenhong
通讯作者	Chen, JX (corresponding author), Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou, Peoples R China. ; Wang, YG (corresponding author), Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China.
来源期刊	PEERJ
ISSN	2167-8359
出版年	2020
卷号	8
英文摘要	Numerous bacteria entered the viable but non-culturable state due to the stresses of dry and salt in soils. YeaZ of Gram-negative bacteria is a resuscitation promoting factor (Rpf) homologous protein could resuscitate bacteria of natural environment in VBNC state. To investigate the promoting effect of YeaZ on the isolation of viable but non-culturable (VBNC) bacteria from soil samples in extreme environments, the recombinant YeaZ of Vibrio harveyi was prepared and added to the soil samples from volcanic soil and saline soil in Northwest China. The study has shown that YeaZ can promote the recovery and growth of soil microorganisms, and the number of cultivable bacteria in volcanic and saline soil has increased from 0.17 x 10(3) and 2.03 x 10(3) cfu.ml(-1) to 1.00 x 10(3) and 5.55 x 10(3) cfu.ml(-1), respectively. The 16S rDNA gene sequencing and phylogenetic analysis showed that YeaZ played an essential role in the increase of composition and diversity of bacteria. A total of 13 bacterial strains were isolated from the volcanic soil samples, which belong to phyla Actinobacteria, Firmicutes and Gamma-proteobacteria. Four species, including Ornithinimicrobium kibberense, Agrococcus citreus, Stenotrophomonas rhizophila and Pseudomonas zhaodongensis were found in the control group, while Micrococcus antarcticus, Kocuria rose, Salinibacterium xinjiangense, Planococcus antarcticus, Ornithinimicrobium kibberense and Pseudomonas zhaodongensis were isolated from the treatment groups (addition of YeaZ). Twentyone strains were isolated from the saline soil samples, including eight species from the control group and thirteen species from the treatment groups, among which nine species were only found, including Bacillus oceanisediminis, Brevibacillus brevis, Paenibacillus xylanilyticus, Microbacterium maritypicum, B. subtilis, B. alcalophilus, B. niabensis, Oceanimonas doudoroffii and Zobellella taiwanensis. The results suggest that addition of YeaZ to soil samples can promote the recovery of VBNC. This method has the implications for the discovery of VBNC bacteria that have potential environmental functions.
英文关键词	Volcano land Saline land VBNC bacteria YeaZ recombinant protein Diversity
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000600832800001
WOS关键词	PROMOTING FACTOR RPF ; MICROBIAL DIVERSITY ; MICROCOCCUS-LUTEUS ; SP NOV. ; DESERT ; STATE ; EXPRESSION ; REVEALS ; LAKE
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349104
作者单位	[Li, Yanlin; Chen, Jixiang; Ma, Dan; Rui, Wenhong] Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou, Peoples R China; [Li, Yanlin] Chongqing Univ Arts & Sci, Coll Chem & Environm Engn, Chongqing Key Lab Environm Mat & Remediat Technol, Chongqing, Peoples R China; [Wang, Yonggang] Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Li, Yanlin,Chen, Jixiang,Wang, Yonggang,et al. The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment[J],2020,8.
APA	Li, Yanlin,Chen, Jixiang,Wang, Yonggang,Ma, Dan,&Rui, Wenhong.(2020).The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment.PEERJ,8.
MLA	Li, Yanlin,et al."The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment".PEERJ 8(2020).