干旱区生态环境知识资源中心(Arid): Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains

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DOI	10.1099/mgen.0.000464
	Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains
	Park, Chulwoo; Shin, Bora; Kim, Wonjae; Cheong, Hoon; Park, Soyoon; Park, Woojun
通讯作者	Park, W (corresponding author), Korea Univ, Dept Environm Sci & Ecol Engn, Lab Mol Environm Microbiol, Seoul 02841, South Korea.
来源期刊	MICROBIAL GENOMICS
ISSN	2057-5858
出版年	2020
卷号	6 期号:12
英文摘要	Deinococcus metallilatus MA1002 was exposed to ultraviolet radiation to generate mutants with enhanced biofilm production. Two strains (nos 5 and 6) were then selected based on their high biofilm formation, as well as their possession of higher concentrations of extracellular matrix components (eDNA, protein and saccharides) than the wild-type (WT). Genomic sequencing revealed the presence of large genome deletions in a secondary chromosome in the mutants. Expression analyses of the WT and mutant strains indicated the upregulation of genes associated with exopolysaccharide synthesis and stress response. The mutant strains showed high mortality in glucose-supplemented (TYG) medium; however, cell death and biofilm formation were not increased in mutant cells grown under acetate- or glyoxylate-added media, suggesting that metabolic toxicity during glucose metabolism induced a high rate of cell death but improved biofilm formation in mutant strains. In damaged cells, eDNAs contributed to the enhanced biofilm formation of D. metallilatus.
英文关键词	biofilm CpG island Deinococcus extracellular matrix genome deletion stress response
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000603125400003
WOS关键词	ENTNER-DOUDOROFF PATHWAY ; SP NOV. ; GEOTHERMALIS BIOFILMS ; RADIATION-RESISTANT ; ISOCITRATE LYASE ; DESICCATION ; RADIODURANS ; MICROCOCCUS ; BACTERIUM ; DESERT
WOS类目	Genetics & Heredity ; Microbiology
WOS研究方向	Genetics & Heredity ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349074
作者单位	[Park, Chulwoo; Shin, Bora; Kim, Wonjae; Cheong, Hoon; Park, Woojun] Korea Univ, Dept Environm Sci & Ecol Engn, Lab Mol Environm Microbiol, Seoul 02841, South Korea; [Park, Soyoon] EMBIOME, Suwon 16614, Gyeonggi, South Korea
推荐引用方式 GB/T 7714	Park, Chulwoo,Shin, Bora,Kim, Wonjae,et al. Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains[J],2020,6(12).
APA	Park, Chulwoo,Shin, Bora,Kim, Wonjae,Cheong, Hoon,Park, Soyoon,&Park, Woojun.(2020).Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains.MICROBIAL GENOMICS,6(12).
MLA	Park, Chulwoo,et al."Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains".MICROBIAL GENOMICS 6.12(2020).