干旱区生态环境知识资源中心(Arid): PCR-based screening and phylogenetic analysis of rat pegivirus (RPgV) carried by rodents in China

Arid

DOI	10.1292/jvms.19-0530
	PCR-based screening and phylogenetic analysis of rat pegivirus (RPgV) carried by rodents in China
	Gao, You-Wen; Wan, Zheng-Wei; Wu, Yue; Li, Xiu-Fen; Tang, Shing-Xing
通讯作者	Tang, SX (corresponding author), Southern Med Univ, Sch Publ Hlth, Dept Epidemiol, 1838 Guangzhou North Rd, Guangzhou 510515, Peoples R China.
来源期刊	JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN	0916-7250
EISSN	1347-7439
出版年	2020
卷号	82 期号:10 页码:1464-1471
英文摘要	Rodent-borne pegiviruses were initially identified in serum samples from desert wood-rats in 2013, and subsequently in serum samples from commensal rats in 2014. However, the prevalence and phylogenetic characteristics of rodent pegiviruses in China are poorly understood. In this study, we screened serum samples collected from wild rats in southern China between 2015 and 2016 for the presence of rat pegivirus (RPgV) by PCR. Among the 314 serum samples from murine rodents (Rattus norvegicus, Rattus tanezumi, and Rattus losea) and house shrews (Suncus murinus), 21.66% (68/314) tested positive for RPgV. Out of these, 23.81% (62/219) of samples from R. norvegicus tested positive, which was significantly higher than that for the other species: 7.69% (1/13), 5.88% (2/34), and 6.25% (3/48) for R. tanezumi, R. losea, and S. murinus, respectively (X-2=18.91, P<0.001). Phylogenetic analysis revealed clustering of viral sequences in the main rodent clade. Analysis of the 3 near-full-length genome sequences of RPgV obtained in this study showed that these viruses exhibited mean nucleic acid and amino acid identities of 94.1% and 98.5% with Chinese RPgV strains, and 90.3 and 97.1% with an RPgV strain from the USA, respectively. This study provides novel insights into the geographic distribution of rodent pegiviruses in China, and identifies potential animal hosts for future studies of these pegiviruses.
英文关键词	epidemiological investigation pegivirus phylogenetic analysis rat shrew
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000607744800010
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349059
作者单位	[Gao, You-Wen; Wan, Zheng-Wei; Wu, Yue; Li, Xiu-Fen; Tang, Shing-Xing] Southern Med Univ, Sch Publ Hlth, Dept Epidemiol, 1838 Guangzhou North Rd, Guangzhou 510515, Peoples R China
推荐引用方式 GB/T 7714	Gao, You-Wen,Wan, Zheng-Wei,Wu, Yue,et al. PCR-based screening and phylogenetic analysis of rat pegivirus (RPgV) carried by rodents in China[J],2020,82(10):1464-1471.
APA	Gao, You-Wen,Wan, Zheng-Wei,Wu, Yue,Li, Xiu-Fen,&Tang, Shing-Xing.(2020).PCR-based screening and phylogenetic analysis of rat pegivirus (RPgV) carried by rodents in China.JOURNAL OF VETERINARY MEDICAL SCIENCE,82(10),1464-1471.
MLA	Gao, You-Wen,et al."PCR-based screening and phylogenetic analysis of rat pegivirus (RPgV) carried by rodents in China".JOURNAL OF VETERINARY MEDICAL SCIENCE 82.10(2020):1464-1471.