干旱区生态环境知识资源中心(Arid): Restriction enzyme optimization for RADseq with camel spiders (Arachnida: Solifugae)

Arid

DOI	10.1636/JoA-S-20-040
	Restriction enzyme optimization for RADseq with camel spiders (Arachnida: Solifugae)
	Santibanez-Lopez, Carlos E.; Farleigh, Keaka; Cushing, Paula E.; Graham, Matthew R.
通讯作者	Santibanez-Lopez, CE (corresponding author), Eastern Connecticut State Univ, Dept Biol, 83 Windham St, Willimantic, CT 06226 USA.
来源期刊	JOURNAL OF ARACHNOLOGY
ISSN	0161-8202
EISSN	1937-2396
出版年	2020
卷号	48 期号:3 页码:346-350
英文摘要	Phylogenetic relationships and evolutionary patterns within the arachnid order Solifugae are poorly understood and largely unresolved due to conserved morphology and scarce genomic resources. In this study, we evaluated the role of restriction endonuclease (RE) selection in double-digest restriction-site-associated DNA sequencing (ddRADseq) as a methodology for exploring the evolutionary history of solifuges and their responses to changing desert landscapes and climate. We optimized this method by using computer simulations to explore the effect of different enzyme combinations on the process in silico. Genome data are not yet available for Solifugae, so we performed the simulations using available spider, scorpion, tick, mite and xiphosuran genomes. Guided by the simulations, we then pioneered ddRADseq in Solifugae by generating data for four samples representing two families and three genera. Our results highlight the utility of simulated data and give us confidence that ddRADseq will be ideal for studying the evolution of solifuges.
英文关键词	3RAD Illumina ddRADseqtools simulations sun spiders wind scorpions
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000621530900017
WOS关键词	PHYLOGENY ; MORPHOLOGY ; SEQUENCE
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348969
作者单位	[Santibanez-Lopez, Carlos E.; Graham, Matthew R.] Eastern Connecticut State Univ, Dept Biol, 83 Windham St, Willimantic, CT 06226 USA; [Farleigh, Keaka] Miami Univ Ohio, Dept Biol, 700 East High St, Oxford, OH 45056 USA; [Cushing, Paula E.] Denver Museum Nat & Sci, Dept Zool, 2001 Colorado Blvd, Denver, CO 80205 USA
推荐引用方式 GB/T 7714	Santibanez-Lopez, Carlos E.,Farleigh, Keaka,Cushing, Paula E.,et al. Restriction enzyme optimization for RADseq with camel spiders (Arachnida: Solifugae)[J],2020,48(3):346-350.
APA	Santibanez-Lopez, Carlos E.,Farleigh, Keaka,Cushing, Paula E.,&Graham, Matthew R..(2020).Restriction enzyme optimization for RADseq with camel spiders (Arachnida: Solifugae).JOURNAL OF ARACHNOLOGY,48(3),346-350.
MLA	Santibanez-Lopez, Carlos E.,et al."Restriction enzyme optimization for RADseq with camel spiders (Arachnida: Solifugae)".JOURNAL OF ARACHNOLOGY 48.3(2020):346-350.