干旱区生态环境知识资源中心(Arid): Analysis of Immune Microenvironment by Multiplex Immunohistochemistry Staining in Different Oral Diseases and Oral Squamous Cell Carcinoma

Arid

DOI	10.3389/fonc.2020.555757
	Analysis of Immune Microenvironment by Multiplex Immunohistochemistry Staining in Different Oral Diseases and Oral Squamous Cell Carcinoma
	Qiao, Bin; Huang, Junwen; Mei, Zi; Lam, Alfred King-yin; Zhao, Junfang; Ying, Le
通讯作者	Zhao, JF (corresponding author), Zhengzhou Univ, Affiliated Hosp 1, Dept Oral & Maxillofacial Surg, Zhengzhou, Peoples R China. ; Ying, L (corresponding author), Hudson Inst Med Res, Ctr Innate Immun & Infect Dis, Clayton, Vic, Australia.
来源期刊	FRONTIERS IN ONCOLOGY
ISSN	2234-943X
出版年	2020
卷号	10
英文摘要	Purpose The aim is to investigate the impacts of using multiplex immunochemistry (mIHC) staining to analyses the co-expression of programmed death ligand-1 (PD-L1) and tumor infiltrating lymphocytes (TILs) [CD8(+) T cells and Forkhead Box Protein 3 (FOXP3)(+) regulatory T cells (Tregs)] in different oral diseases, and oral squamous cell carcinoma (OSCC). Methods Formalin fixed paraffin-embedded tissue sections from different oral diseases were stained with PD-L1 and TILs (CD8(+) T cells and FOXP3(+) Tregs) by mIHC staining simultaneously. The whole slide was scanned digitally to observe the cell phenotypes stained in the microenvironment. The contents of each slice were read using a computer-aided method to analyze and the cell densities were calculated using statistical software. Results We were able to characterize the tumor microenvironment (TME) of different oral diseases including oral leukoplakia (OLK), inflammatory gingiva (IG), oral lichen planus (OLP), and squamous cell carcinoma (SCC), with accurate visualization of various immune cells harboring complex immune phenotypes by mIHC staining. The results showed that PD-L1 was up-regulated in SCC tissues at different pathological stages, while CD8 and FOXP3 had no significant changes. The ratio of PD-L1/CD8 was also significantly up-regulated in SCC tissues compared with that of other oral diseases. In advanced stages of OSCC, the FOXP3/CD8 ratio increased, and immunosuppressive TME was more pronounced. In addition, we also found different immune phenotypes: the inflamed phenotype, immune-excluded phenotypes, and immune-desert phenotypes. By locating tumor epithelial cells, we found that PD-L1 expression is in both tumor cells and stromal cells. Conclusions mIHC is useful for the visualization and evaluation of tumor microenvironment in immuno-oncology research. It allows single-cell imaging in situ and could effectively and quickly determine the immune phenotype of different oral diseases.
英文关键词	oral squamous cell carcinoma multiplex immunochemistry PD-L1 oral disease CD8
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000600760300001
WOS关键词	TUMOR-INFILTRATING LYMPHOCYTES ; T-CELLS ; CANCER ; QUANTIFICATION ; IMMUNOTHERAPY ; IMPACT
WOS类目	Oncology
WOS研究方向	Oncology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348867
作者单位	[Qiao, Bin; Huang, Junwen; Mei, Zi; Zhao, Junfang] Zhengzhou Univ, Affiliated Hosp 1, Dept Oral & Maxillofacial Surg, Zhengzhou, Peoples R China; [Lam, Alfred King-yin] Griffith Univ, Canc Mol Pathol & Griffith Med Sch, Gold Coast, Qld, Australia; [Ying, Le] Hudson Inst Med Res, Ctr Innate Immun & Infect Dis, Clayton, Vic, Australia
推荐引用方式 GB/T 7714	Qiao, Bin,Huang, Junwen,Mei, Zi,et al. Analysis of Immune Microenvironment by Multiplex Immunohistochemistry Staining in Different Oral Diseases and Oral Squamous Cell Carcinoma[J],2020,10.
APA	Qiao, Bin,Huang, Junwen,Mei, Zi,Lam, Alfred King-yin,Zhao, Junfang,&Ying, Le.(2020).Analysis of Immune Microenvironment by Multiplex Immunohistochemistry Staining in Different Oral Diseases and Oral Squamous Cell Carcinoma.FRONTIERS IN ONCOLOGY,10.
MLA	Qiao, Bin,et al."Analysis of Immune Microenvironment by Multiplex Immunohistochemistry Staining in Different Oral Diseases and Oral Squamous Cell Carcinoma".FRONTIERS IN ONCOLOGY 10(2020).