干旱区生态环境知识资源中心(Arid): A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration

Arid

DOI	10.3389/fmicb.2020.590736
	A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration
	Gallardo-Carreno, Ignacio; Moreno-Paz, Mercedes; Aguirre, Jacobo; Blanco, Yolanda; Alonso-Pintado, Eduardo; Raymond-Bouchard, Isabelle; Maggiori, Catherine; Rivas, Luis A.; Engelbrektson, Anna; Whyte, Lyle; Parro, Victor
通讯作者	Moreno-Paz, M (corresponding author), Ctr Astrobiol INTA CSIC, Dept Mol Evolut, Madrid, Spain.
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2020
卷号	11
英文摘要	Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the surface of Mars. As some bacterial strains are capable of using perchlorate as an electron acceptor under anaerobic conditions, their detection is relevant for environmental monitoring on Earth as well as for the search for life on Mars. We have developed an antibody microarray with 20 polyclonal antibodies to detect perchlorate-reducing bacteria (PRB) strains and two crucial and highly conserved enzymes involved in perchlorate respiration: perchlorate reductase and chlorite dismutase. We determined the cross-reactivity, the working concentration, and the limit of detection of each antibody individually and in a multiplex format by Fluorescent Sandwich Microarray Immunoassay. Although most of them exhibited relatively high sensitivity and specificity, we applied a deconvolution method based on graph theory to discriminate between specific signals and cross-reactions from related microorganisms. We validated the system by analyzing multiple bacterial isolates, crude extracts from contaminated reactors and salt-rich natural samples from the high Arctic. The PRB detecting chip (PRBCHIP) allowed us to detect and classify environmental isolates as well as to detect similar strains by using crude extracts obtained from 0.5 g even from soils with low organic-matter levels (<10(3) cells/g of soil). Our results demonstrated that PRBCHIP is a valuable tool for sensitive and reliable detection of perchlorate-reducing bacteria for research purposes, environmental monitoring and planetary exploration.
英文关键词	perchlorate perchlorate-reducing bacteria antibody microarrays biochip life detection planetary exploration Mars graph theory
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000603461400001
WOS关键词	UNEXPECTED DIVERSITY ; MICROBIAL COMMUNITY ; CHLORITE DISMUTASES ; GALE CRATER ; MARS ; SEARCH ; (PER)CHLORATE ; REDUCTION ; PHOENIX ; MISSION
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	University of California, Berkeley
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348865
作者单位	[Gallardo-Carreno, Ignacio; Moreno-Paz, Mercedes; Aguirre, Jacobo; Blanco, Yolanda; Alonso-Pintado, Eduardo; Rivas, Luis A.; Parro, Victor] Ctr Astrobiol INTA CSIC, Dept Mol Evolut, Madrid, Spain; [Aguirre, Jacobo] CSIC, Ctr Nacl Biotecnol, Madrid, Spain; [Aguirre, Jacobo] Grp Interdisciplinar Sistemas Complejos GISC, Madrid, Spain; [Raymond-Bouchard, Isabelle; Maggiori, Catherine; Whyte, Lyle] McGill Univ, Dept Nat Resource Sci, Ste Anne De Bellevue, PQ, Canada; [Rivas, Luis A.] Inmunol & Genet Aplicada SA INGENASA, Madrid, Spain; [Engelbrektson, Anna] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
推荐引用方式 GB/T 7714	Gallardo-Carreno, Ignacio,Moreno-Paz, Mercedes,Aguirre, Jacobo,et al. A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration[J]. University of California, Berkeley,2020,11.
APA	Gallardo-Carreno, Ignacio.,Moreno-Paz, Mercedes.,Aguirre, Jacobo.,Blanco, Yolanda.,Alonso-Pintado, Eduardo.,...&Parro, Victor.(2020).A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.FRONTIERS IN MICROBIOLOGY,11.
MLA	Gallardo-Carreno, Ignacio,et al."A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration".FRONTIERS IN MICROBIOLOGY 11(2020).