干旱区生态环境知识资源中心(Arid): Elevated Levels of Oxidative Nucleic Acid Modification Markers in Urine From Gastric Cancer Patients: Quantitative Analysis by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Arid

DOI	10.3389/fchem.2020.606495
	Elevated Levels of Oxidative Nucleic Acid Modification Markers in Urine From Gastric Cancer Patients: Quantitative Analysis by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
	Chen, Qin; Hu, Yiqiu; Fang, Zhihao; Ye, Minfeng; Li, Jingqing; Zhang, Suzhan; Yuan, Ying; Guo, Cheng
通讯作者	Yuan, Y ; Guo, C (corresponding author), Zhejiang Univ, Key Lab Canc Prevent & Intervent, China Natl Minist Educ, Canc Inst,Affiliated Hosp 2,Sch Med, Hangzhou, Peoples R China. ; Yuan, Y (corresponding author), Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Med Oncol, Hangzhou, Peoples R China.
来源期刊	FRONTIERS IN CHEMISTRY
ISSN	2296-2646
出版年	2020
卷号	8
英文摘要	Oxidative nucleic acid modifications have attracted increasing attention in recent years since they have been found to be related to a number of diseases including cancer. 8-Hydroxy-2 '-deoxyguanosine (8-OHdG) and 8-hydroxyguanosine (8-OHG) are the typical markers of oxidative modification of DNA and RNA, respectively, and they are emerging biomarkers for the early detection of diseases. Urine is a favored biofluid for biomarker discovery due to its noninvasiveness to patients. Accurate quantification of these oxidative nucleic acid modifications still has challenges because their amounts in urine are very low and the interferences in urine samples are complicated. Herein, we developed and validated an accurate and robust solid-phase extraction (SPE) coupled with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous quantification of these oxidative nucleic acid modifications in human urine. Stable isotope dilution strategy was utilized and the method shows good precision on intraday and interday measurements. Meanwhile, recovery was satisfactory by utilizing the Oasis hydrophilic-lipophilic balance (HLB) cartridge for sample pretreatment at three spiked levels. We successfully quantified urinary 8-OHdG and 8-OHG from 60 gastric cancer patients and 70 healthy controls by using this method. The measured contents of 8-OHdG and 8-OHG in urine from gastric cancer patients are both increased, compared with those in urine from healthy controls, indicating these oxidative nucleic acid modifications could act as potential non-invasive markers for early diagnosis of gastric cancer. Moreover, the present study will stimulate investigations of the effects of oxidative stress and nucleic acid modifications on the initiation and progression of gastric cancer.
英文关键词	UPLC-MS MS nucleic acid modification oxidative stress gastric cancer biomarker urine
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000603615900001
WOS关键词	SOLID-PHASE EXTRACTION ; RNA MODIFICATIONS ; GENE-EXPRESSION ; DNA ; BIOMARKER ; QUANTIFICATION
WOS类目	Chemistry, Multidisciplinary
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348859
作者单位	[Chen, Qin; Hu, Yiqiu; Fang, Zhihao; Li, Jingqing; Zhang, Suzhan; Yuan, Ying; Guo, Cheng] Zhejiang Univ, Key Lab Canc Prevent & Intervent, China Natl Minist Educ, Canc Inst,Affiliated Hosp 2,Sch Med, Hangzhou, Peoples R China; [Ye, Minfeng] Zhejiang Univ, Dept Gastrointestinal Surg, Shaoxing Peoples Hosp, Shaoxing Hosp, Shaoxing, Peoples R China; [Li, Jingqing] Zhejiang Univ, Sch Med, Zhejiang Univ Univ Edinburgh Inst, Haining, Peoples R China; [Yuan, Ying] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Med Oncol, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Chen, Qin,Hu, Yiqiu,Fang, Zhihao,et al. Elevated Levels of Oxidative Nucleic Acid Modification Markers in Urine From Gastric Cancer Patients: Quantitative Analysis by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J],2020,8.
APA	Chen, Qin.,Hu, Yiqiu.,Fang, Zhihao.,Ye, Minfeng.,Li, Jingqing.,...&Guo, Cheng.(2020).Elevated Levels of Oxidative Nucleic Acid Modification Markers in Urine From Gastric Cancer Patients: Quantitative Analysis by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry.FRONTIERS IN CHEMISTRY,8.
MLA	Chen, Qin,et al."Elevated Levels of Oxidative Nucleic Acid Modification Markers in Urine From Gastric Cancer Patients: Quantitative Analysis by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry".FRONTIERS IN CHEMISTRY 8(2020).