干旱区生态环境知识资源中心(Arid): Study on Neuroendocrine-Immune Function of Cistanche deserticola and Its Rice Wine Steaming Products in Glucocorticoid-Induced Rat Model

Arid

DOI	10.1155/2020/5321976
	Study on Neuroendocrine-Immune Function of Cistanche deserticola and Its Rice Wine Steaming Products in Glucocorticoid-Induced Rat Model
	Liu, Bonan; Shi, Ji; Li, Zhe; Zhang, Chao; Liu, Pengpeng; Yao, Wei; Jia, Tianzhu
通讯作者	Shi, J (corresponding author), Liaoning Univ Tradit Chinese Med, Sch Pharm, Dalian 116600, Peoples R China. ; Yao, W (corresponding author), Dalian Med Univ, Hosp 2, Dalian 116600, Peoples R China.
来源期刊	EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
ISSN	1741-427X
EISSN	1741-4288
出版年	2020
卷号	2020
英文摘要	The desert-dwelling Cistanche herb was first recorded in the Shen Nong Herbal Classic and is listed as the top-grade herbal medicine in this publication. The Chinese Pharmacopoeia records that pieces of Cistanche deserticola (CD) and rice wine-steamed Cistanche deserticola (WCD) can be used in the clinic as the main types of decoctions. After being steamed with rice wine, the antiaging and tonifying kidney-yang effects are enhanced. In this study, we detected the chemical content of CD and WCD and the pharmacological mechanism of invigorating kidney-yang deficiency in model rats. Aim. The purpose of this study was to examine the effects of CD and WCD on the neuroendocrine-immune function of kidney-yang deficiency in glucocorticoid-overdosed model rats. Materials and methods. Sprague Dawley (SD) rats were selected. The rats were subcutaneously injected with corticosterone water suspension for the glucocorticoid-overdosed model rats. The positive control rats were gavaged with Jinkuishenqi pills and high-, medium-, and low-dose CD/WCD suspensions (1.646 g/(kg day), 5.48 g/(kg day), 2.74 g/(kg day), and 1.37 g/(kg day), respectively); the blank control (BC) and model control (MC) groups were given the same volume of distilled water as those in the drug group for 40 consecutive days at a dose of 1 mL/100 g. After the last administration, the blood was collected from the abdominal aorta, and serum levels of T, CRH, ACTH, CORT, cortisol, IL-10, IL-6, IL-2, TNF-alpha, and IFN-gamma were measured. Organ indexes of the thymus gland and the spleen were calculated. The expression of Bax, Bcl-2, caspase-3, Fas, and FasL in the adrenal gland was measured by immunohistochemistry. The pathological changes in the thymus gland and the adrenal gland were observed by HE staining (x200). T lymphocyte subsets in peripheral blood were detected by flow cytometry, and the expression of CaM mRNA in the hypothalamus and hypophysis tissues was also measured by RT-PCR. Results. Compared with the MC group, the CD and WCD groups exhibited increases in activity, the organ index of the thymus and the spleen, the serum levels of T, CRH, ACTH, CORT, cortisol, IL-2, and IL-10, the ratio of CD4+/CD8+, and the expression of Bcl-2, caspase-3, Fas, FasL, and CaM in the hypophysis tissue. The CD and WCD groups also exhibited reductions in the IL-6, TNF-alpha, and IFN-gamma levels in serum and the expression of CaM mRNA in the hypothalamus. Conclusions. Each dose of CD and WCD could counteract the dysregulated sex hormone and immune factors in glucocorticoid-overdosed model rats, enhancing and restoring the effect of the hypothalamic nerve cells and improving immune function.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000607902500005
WOS关键词	ECHINACOSIDE ; APOPTOSIS ; MICE
WOS类目	Integrative & Complementary Medicine
WOS研究方向	Integrative & Complementary Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348848
作者单位	[Liu, Bonan; Shi, Ji; Li, Zhe; Zhang, Chao; Liu, Pengpeng; Jia, Tianzhu] Liaoning Univ Tradit Chinese Med, Sch Pharm, Dalian 116600, Peoples R China; [Yao, Wei] Dalian Med Univ, Hosp 2, Dalian 116600, Peoples R China
推荐引用方式 GB/T 7714	Liu, Bonan,Shi, Ji,Li, Zhe,et al. Study on Neuroendocrine-Immune Function of Cistanche deserticola and Its Rice Wine Steaming Products in Glucocorticoid-Induced Rat Model[J],2020,2020.
APA	Liu, Bonan.,Shi, Ji.,Li, Zhe.,Zhang, Chao.,Liu, Pengpeng.,...&Jia, Tianzhu.(2020).Study on Neuroendocrine-Immune Function of Cistanche deserticola and Its Rice Wine Steaming Products in Glucocorticoid-Induced Rat Model.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2020.
MLA	Liu, Bonan,et al."Study on Neuroendocrine-Immune Function of Cistanche deserticola and Its Rice Wine Steaming Products in Glucocorticoid-Induced Rat Model".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2020(2020).