干旱区生态环境知识资源中心(Arid): Seasonality Modulates the Cellular Antioxidant Activity and Antiproliferative Effect of Sonoran Desert Propolis

Arid

DOI	10.3390/antiox9121294
	Seasonality Modulates the Cellular Antioxidant Activity and Antiproliferative Effect of Sonoran Desert Propolis
	Mendez-Pfeiffer, Pablo; Alday, Efrain; Carreno, Ana Laura; Hernandez-Tanori, Jorge; Montano-Leyva, Beatriz; Ortega-Garcia, Jesus; Valdez, Judith; Garibay-Escobar, Adriana; Hernandez, Javier; Valencia, Dora; Velazquez, Carlos
通讯作者	Velazquez, C (corresponding author), Univ Sonora, Dept Chem Biol, Blvd Luis Encinas & Rosales S-N, Hermosillo 83000, Sonora, Mexico. ; Valencia, D (corresponding author), Univ Sonora, Dept Chem Biol & Agropecuary Sci, Av Univ & Irigoyen, Caborca 83600, Sonora, Mexico.
来源期刊	ANTIOXIDANTS
EISSN	2076-3921
出版年	2020
卷号	9 期号:12
英文摘要	The main chemical composition and pharmacological potential of propolis from arid and semi-arid regions of the Sonoran Desert have been previously reported. Caborca propolis (CP), from an arid zone of the Sonoran Desert, has shown a polyphenolic profile that suggests a mixed plant origin, presenting poplar-type markers, as well as a 6-methoxylated flavonoid, xanthomicrol, characteristic of Asteraceae plants. In addition, CP has shown significant antioxidant properties and antiproliferative activity on cancer cells. In this study, we analyzed the influence of collection time on the chemical constitution, antiproliferative activity and protective capacity of CP against reactive oxygen species (ROS), by using HPLC-UV-diode array detection (DAD) analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-Dimethyltetrazoliumbromide (MTT) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays, as well as cellular antioxidant activity (CAA) assay on murine B-cell lymphoma M12.C3.F6 cells. HPLC-UV-DAD analyses of seasonally collected CP (one-year period) revealed quantitative differences among the most abundant CP constituents: pinocembrin, galangin, chrysin and pinobanksin-3-O-acetate. Though all seasonal samples of CP induced an antiproliferative effect in M12.C3.F6 cells, CP from autumn showed the highest inhibitory activity (IC50: 5.9 +/- 0.6 mu g/mL). The DPPH assay pointed out that CP collected in autumn presented the highest antioxidant potential (IC50: 58.8 +/- 6.7 mu g/mL), followed by winter (65.7 +/- 12.2 mu g/mL) and spring (67.0 +/- 7.5 mu g/mL); meanwhile, the summer sample showed a lesser antioxidant capacity (IC50: 98.7 +/- 2.5 mu g/mL). The CAA assay demonstrated that CP induced a significant protective effect against ROS production elicited by H2O2 in M12.C3.F6 cells. Pretreatment of M12.C3.F6 cells with CP from spring and autumn (25 and 50 mu g/mL for 1 h) showed the highest reduction in intracellular ROS induced by H2O2 (1 and 5 mM). These results indicate that the antiproliferative effect and cellular antioxidant activity of CP are modulated by quantitative fluctuations in its polyphenolic profile due to its collection time.
英文关键词	mixed poplar-type propolis polyphenolic composition antiproliferative effect cellular antioxidant activity seasonal effect
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000601866100001
WOS关键词	CHEMICAL-COMPOSITION ; HONEY-BEES ; ANTIBACTERIAL ; ASSAY ; ROS ; CONSTITUENTS ; GROWTH ; CELLS
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Food Science & Technology
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348714
作者单位	[Mendez-Pfeiffer, Pablo; Alday, Efrain; Carreno, Ana Laura; Valdez, Judith; Garibay-Escobar, Adriana; Velazquez, Carlos] Univ Sonora, Dept Chem Biol, Blvd Luis Encinas & Rosales S-N, Hermosillo 83000, Sonora, Mexico; [Hernandez-Tanori, Jorge; Ortega-Garcia, Jesus; Valencia, Dora] Univ Sonora, Dept Chem Biol & Agropecuary Sci, Av Univ & Irigoyen, Caborca 83600, Sonora, Mexico; [Montano-Leyva, Beatriz] Univ Sonora, Dept Invest & Posgrad Alimentos, Blvd Luis Encinas & Rosales S-N, Hermosillo 83000, Sonora, Mexico; [Hernandez, Javier] Univ Veracruzana, Unidad Serv Apoyo Resoluc Analit, Xalapa 91190, Veracruz, Mexico
推荐引用方式 GB/T 7714	Mendez-Pfeiffer, Pablo,Alday, Efrain,Carreno, Ana Laura,et al. Seasonality Modulates the Cellular Antioxidant Activity and Antiproliferative Effect of Sonoran Desert Propolis[J],2020,9(12).
APA	Mendez-Pfeiffer, Pablo.,Alday, Efrain.,Carreno, Ana Laura.,Hernandez-Tanori, Jorge.,Montano-Leyva, Beatriz.,...&Velazquez, Carlos.(2020).Seasonality Modulates the Cellular Antioxidant Activity and Antiproliferative Effect of Sonoran Desert Propolis.ANTIOXIDANTS,9(12).
MLA	Mendez-Pfeiffer, Pablo,et al."Seasonality Modulates the Cellular Antioxidant Activity and Antiproliferative Effect of Sonoran Desert Propolis".ANTIOXIDANTS 9.12(2020).