干旱区生态环境知识资源中心(Arid): A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging

Arid

DOI	10.1016/j.plantsci.2020.110765
	A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging
	Zhao, Daqiu; Luan, Yuting; Shi, Wenbo; Zhang, Xiayan; Meng, Jiasong; Tao, Jun
通讯作者	Tao, J (corresponding author), Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China.
来源期刊	PLANT SCIENCE
ISSN	0168-9452
EISSN	1873-2259
出版年	2021
卷号	303
英文摘要	Paeonia ostii is an emerging woody oil crop, but drought severely inhibits its growth and promotion in arid or semiarid areas, and little is known about the mechanism governing this inhibition. In this study, the full-length cDNA of a caffeoyl-CoA O-methyltransferase gene (CCoAOMT) from P. ostii was isolated, and determined to be comprised of 987 bp. PoCCoAOMT encoded a 247-amino acid protein, which was located in the nucleus and cytosol. Significantly higher PoCCoAOMT transcription was detected in P. ostii treated with drought stress. Subsequently, the constitutive overexpression of PoCCoAOMT in tobacco significantly conferred drought stress tolerance. Under drought stress, transgenic lines exhibited lower reactive oxygen species (ROS) accumulation, and higher antioxidant enzyme activities and photosynthesis. Moreover, the expression levels of senescence-associated genes were significantly downregulated, whereas the expression levels of lignin biosynthetic genes and PoCCoAOMT were significantly upregulated in transgenic lines. Similarly, transgenic lines produced significantly higher lignin, especially guaiacyl-lignin. These results suggest that PoCCoAOMT is a vital gene in promoting lignin synthesis and ROS scavenging to confer drought stress tolerance in P. ostii.
英文关键词	Oil peony Abiotic stress Drought-resistant genes Guaiacyl-lignin
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000614153000004
WOS关键词	TRANSCRIPTIONAL ANALYSIS ; HIGH-TEMPERATURE ; DOWN-REGULATION ; BIOSYNTHESIS ; COENZYME ; CLONING ; 3-O-METHYLTRANSFERASE ; EXPRESSION ; L. ; CONDUCTANCE
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348097
作者单位	[Zhao, Daqiu; Luan, Yuting; Shi, Wenbo; Zhang, Xiayan; Meng, Jiasong; Tao, Jun] Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China; [Tao, Jun] Yangzhou Univ, Joint Int Res Lab Agr & Agri Prod Safety, Minist Educ China, Yangzhou 225009, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Daqiu,Luan, Yuting,Shi, Wenbo,et al. A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging[J],2021,303.
APA	Zhao, Daqiu,Luan, Yuting,Shi, Wenbo,Zhang, Xiayan,Meng, Jiasong,&Tao, Jun.(2021).A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging.PLANT SCIENCE,303.
MLA	Zhao, Daqiu,et al."A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging".PLANT SCIENCE 303(2021).