干旱区生态环境知识资源中心(Arid): Desert Environments Facilitate Unique Evolution of Biosynthetic Potential in Streptomyces

Arid

DOI	10.3390/molecules26030588
	Desert Environments Facilitate Unique Evolution of Biosynthetic Potential in Streptomyces
	Sivakala, Kunjukrishnan Kamalakshi; Gutierrez-Garcia, Karina; Jose, Polpass Arul; Thinesh, Thangadurai; Anandham, Rangasamy; Barona-Gomez, Francisco; Sivakumar, Natesan
通讯作者	Sivakumar, N (corresponding author), Madurai Kamaraj Univ, Sch Biotechnol, Madurai 625021, Tamil Nadu, India. ; Jose, PA (corresponding author), Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, IL-7610001 Rehovot, Israel. ; Jose, PA (corresponding author), Tamil Nadu Agr Univ, Dept Agr Microbiol, Agr Coll & Res Inst, Madurai 625104, Tamil Nadu, India.
来源期刊	MOLECULES
EISSN	1420-3049
出版年	2021
卷号	26 期号:3
英文摘要	Searching for new bioactive metabolites from the bacterial genus Streptomyces is a challenging task. Combined genomic tools and metabolomic screening of Streptomyces spp. native to extreme environments could be a promising strategy to discover novel compounds. While Streptomyces of desertic origin have been proposed as a source of new metabolites, their genome mining, phylogenetic analysis, and metabolite profiles to date are scarcely documented. Here, we hypothesized that Streptomyces species of desert environments have evolved with unique biosynthetic potential. To test this, along with an extensive characterization of biosynthetic potential of a desert isolate Streptomyces sp. SAJ15, we profiled phylogenetic relationships among the closest and previously reported Streptomyces of desert origin. Results revealed that Streptomyces strains of desert origin are closer to each other and relatively distinct from Streptomyces of other environments. The draft genome of strain SAJ15 was 8.2 Mb in size, which had 6972 predicted genes including 3097 genes encoding hypothetical proteins. Successive genome mining and phylogenetic analysis revealed the presence of putative novel biosynthetic gene clusters (BGCs) with low incidence in another Streptomyces. In addition, high-resolution metabolite profiling indicated the production of arylpolyene, terpenoid, and macrolide compounds in an optimized medium by strain SAJ15. The relative abundance of different BGCs in arid Streptomyces differed from the non-arid counterparts. Collectively, the results suggested a distinct evolution of desert Streptomyces with a unique biosynthetic potential.
英文关键词	actinobacteria genome mining metabolomics secondary metabolites natural products arid environment
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000615435800001
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
来源机构	Hebrew University of Jerusalem
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348069
作者单位	[Sivakala, Kunjukrishnan Kamalakshi; Sivakumar, Natesan] Madurai Kamaraj Univ, Sch Biotechnol, Madurai 625021, Tamil Nadu, India; [Sivakala, Kunjukrishnan Kamalakshi; Jose, Polpass Arul] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, IL-7610001 Rehovot, Israel; [Gutierrez-Garcia, Karina; Barona-Gomez, Francisco] Cinvestav IPN, Evolut Metab Divers Lab, Unidad Genom Avanzada Langebio, Km 9-6 Libramiento Norte,Carretera Irapuato Leon, Guanajuato 36821, Mexico; [Gutierrez-Garcia, Karina] Carnegie Inst Sci, Dept Embryol, Baltimore, MD 21218 USA; [Jose, Polpass Arul; Anandham, Rangasamy] Tamil Nadu Agr Univ, Dept Agr Microbiol, Agr Coll & Res Inst, Madurai 625104, Tamil Nadu, India; [Thinesh, Thangadurai] Pondicherry Univ, Sch Life Sci, Dept Microbiol, Pondicherry 605014, India
推荐引用方式 GB/T 7714	Sivakala, Kunjukrishnan Kamalakshi,Gutierrez-Garcia, Karina,Jose, Polpass Arul,et al. Desert Environments Facilitate Unique Evolution of Biosynthetic Potential in Streptomyces[J]. Hebrew University of Jerusalem,2021,26(3).
APA	Sivakala, Kunjukrishnan Kamalakshi.,Gutierrez-Garcia, Karina.,Jose, Polpass Arul.,Thinesh, Thangadurai.,Anandham, Rangasamy.,...&Sivakumar, Natesan.(2021).Desert Environments Facilitate Unique Evolution of Biosynthetic Potential in Streptomyces.MOLECULES,26(3).
MLA	Sivakala, Kunjukrishnan Kamalakshi,et al."Desert Environments Facilitate Unique Evolution of Biosynthetic Potential in Streptomyces".MOLECULES 26.3(2021).