干旱区生态环境知识资源中心(Arid): Identifying the Biogeographic Patterns of Rare and Abundant Bacterial Communities Using Different Primer Sets on the Loess Plateau

Arid

DOI	10.3390/microorganisms9010139
	Identifying the Biogeographic Patterns of Rare and Abundant Bacterial Communities Using Different Primer Sets on the Loess Plateau
	Zeng, Quanchao; An, Shaoshan
通讯作者	An, SS (corresponding author), Northwest A&F Univ, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China.
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2021
卷号	9 期号:1
英文摘要	High-throughput sequencing is commonly used to study soil microbial communities. However, different primers targeting different 16S rRNA hypervariable regions often generate different microbial communities and result in different values of diversity and community structure. This study determined the consequences of using two bacterial primers (338f/806r, targeting the V3-V4 region, and 520f/802r, targeting the V4 region) to assess bacterial communities in the soils of different land uses along a latitudinal gradient. The results showed that the variations in the soil bacterial diversity in different land uses were more evident based on the former pair. The statistical results showed that land use had no significant impact on soil bacterial diversity when primer pair 520f/802r was used. In contrast, when primer pair 338f/806r was used, the cropland and orchard soils had significantly higher operational taxonomic units (OTUs) and Shannon diversity index values than those of the shrubland and grassland soils. Similarly, the soil bacterial diversity generated by primer pair 338f/806r was significantly impacted by mean annual precipitation, soil total phosphorus (TP), soil total nitrogen (TN), and soil available phosphorus (AVP), while the soil bacterial diversity generated by primer pair 520f/802r showed no significant correlations with most of these environmental factors. Multiple regression models indicated that soil pH and soil organic carbon (SOC) shaped the soil bacterial community structure on the Loess Plateau regardless of what primer pair was used. Climatic conditions mainly affected the diversity of rare bacteria. Abundant bacteria are more sensitive than rare bacteria to environmental changes. Very little of the variation in the rare bacterial community was explained by environmental factors or geographic distance, suggesting that the communities of rare bacteria are unpredictable. The distributions of the abundant taxa were mainly determined by variations in environmental factors.
英文关键词	primer sets rare bacteria spatial patterns arid regions land uses
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000610586800001
WOS关键词	SOIL MICROBIAL COMMUNITIES ; CARBON CONTENT ; DIVERSITY ; CONVERSION ; RESPONSES ; CLASSIFICATION ; VEGETATION ; PH
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/348063
作者单位	[Zeng, Quanchao] Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Peoples R China; [Zeng, Quanchao; An, Shaoshan] Northwest A&F Univ, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Quanchao,An, Shaoshan. Identifying the Biogeographic Patterns of Rare and Abundant Bacterial Communities Using Different Primer Sets on the Loess Plateau[J]. 西北农林科技大学,2021,9(1).
APA	Zeng, Quanchao,&An, Shaoshan.(2021).Identifying the Biogeographic Patterns of Rare and Abundant Bacterial Communities Using Different Primer Sets on the Loess Plateau.MICROORGANISMS,9(1).
MLA	Zeng, Quanchao,et al."Identifying the Biogeographic Patterns of Rare and Abundant Bacterial Communities Using Different Primer Sets on the Loess Plateau".MICROORGANISMS 9.1(2021).